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The Writer of Modern Life: Essays on Charles Baudelaire
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The Writer of Modern Life: Essays on Charles Baudelaire by Walter
louis pasteur essay By Louis Pasteur. F. Faulkner And D. C. Robb. By Mm. Pasteur, Jourbert, And Chamberland. Professor Of Bacteriology In The Harvard Medical School. On The Extension Of The Germ Theory To The Etiology Of Certain. By Louis Pasteur. Translated By H. C. Life. Ernst, M. D. Louis Pasteur was born at Dole, Jura, France, December 27, 1822, and died near Saint-Cloud, September 28, 1895.
His interest in science, and especially in chemistry, developed early, and by the time he was twenty-six he was professor of the physical sciences at Dijon. General Formula Chemosynthesis. The most important academic positions held by of modern life essays baudelaire him later were those as professor of chemistry at Strasburg, 1849; dean of the Faculty of solving, Sciences at Lille, 1854; science director of the Ecole Normale Superieure, Paris, 1857; professor of geology, physics, and chemistry at the Ecole des Beaux Arts; Professor of chemistry at the Sorbonne, 1867. After 1875 he carried on life essays baudelaire his researches at the Pasteur Institute. Solving With For Free. He was a member of the Institute, and received many honors from learned societies at home and abroad. In respect of the number and importance, practical as well as scientific, of his discoveries, Pasteur has hardly a rival in the history of science. He may be regarded as the founder of modern stereo-chemistry; and his discovery that living organisms are the cause of fermentation is the basis of the whole modern germ- theory of disease and of the antiseptic method of treatment. His investigations of the the writer life, diseases of beer and wine; of to write essay, pebrine, a disease affecting silk-worms; of anthrax, and of fowl cholera, were of life baudelaire, immense commercial importance and ways to write an argumentative led to conclusions which have revolutionised physiology, pathology, and life on charles baudelaire therapeutics. General Chemosynthesis. By his studies in the culture of bacteria of attenuated virulence he extended widely the practise of inoculation with a milder form of various diseases, with a view to producing immunity. The following papers present some of the most important of his contributions, and exemplify his extraordinary powers of lucid exposition and argument. TO THE MEMORY OF MY FATHER FORMERLY A SOLDIER UNDER THE FIRST EMPIRE CHEVALIER OF THE LEGION OF HONOR. The longer I live, the better I understand the kindness of thy heart and the high quality of thy mind.
The efforts which I have devoted to these Studies, as well as those which preceded them, are the fruit of thy counsel and example. Desiring to honor these filial remembrances, I dedicate this work to thy memory. Our misfortunes inspired me with the idea of these researches. I undertook them immediately after the war of 1870, and have since continued them without interruption, with the determination of perfecting them, and thereby benefiting a branch of industry wherein we are undoubtedly surpassed by Germany. I am convinced that I have found a precise, practical solution of the arduous problem which I proposed to myself--that of a process of manufacture, independent of season and locality, which should obviate the necessity of having recourse to the costly methods of cooling employed in existing processes, and at the same time secure the preservation of its products for the writer of modern essays baudelaire, any length of time.
These new studies are based on the same principles which guided me in my researches on different ways wine, vinegar, and the silkworm disease-- principles, the applications of which are practically unlimited. The etiology of the writer life essays baudelaire, contagious diseases may, perhaps, receive from them an lotf microcosm, unexpected light. I need not hazard any prediction concerning the advantages likely to accrue to the writer of modern life on charles baudelaire the brewing industry from the adoption of such a process of family, brewing as my study of the subject has enabled me to devise, and from an application of the novel facts upon the writer of modern on charles, which this process is founded. Time is the best appraiser of essay, scientific work, and I am not unaware that an the writer essays, industrial discovery rarely produces all its fruit in the hands of its first inventor. I began my researches at Clermont-Ferrand, in the laboratory, and with the help, of my friend M. Duclaux, professor of math problems for free, chemistry at the Faculty of Sciences of that town. I continued them in Paris, and of modern afterwards at the great brewery of general formula chemosynthesis, Tourtel Brothers, of Tantonville, which is admitted to be the first in France. I heartily thank these gentlemen for the writer, their extreme kindness. I owe also a public tribute of gratitude to M. Kuhn, a skillful brewer of Chamalieres, near Clermont-Ferrand, as well as to different M. The Writer Life Essays. Velten of Marseilles, and to MM. de Tassigny, of language, Reims, who have placed at my disposal their establishments and their products, with the most praiseworthy eagerness. Paris, June 1, 1879. THE PHYSIOLOGICAL THEORY OF FERMENTATION - TOC.
I. ON THE RELATIONS EXISTING BETWEEN OXYGEN AND YEAST. It is characteristic of science to reduce incessantly the number of unexplained phenomena. It is observed, for instance, that fleshy fruits are not liable to fermentation so long as their epidermis remains uninjured. On the other hand, they ferment very readily when they are piled up in heaps more or less open, and immersed in their saccharine juice. The mass becomes heated and swells; carbonic acid gas is disengaged, and the sugar disappears and is replaced by alcohol. Now, as to the question of the origin of these spontaneous phenomena, so remarkable in character as well as usefulness for the writer of modern life, man's service, modern knowledge has taught us that fermentation is the consequence of a development of vegetable cells the germs of which do not exist in essay, the saccharine juices within fruits; that many varieties of these cellular plants exist, each giving rise to its own particular fermentation. The Writer Essays On Charles. The principal products of chemosynthesis, these various fermentations, although resembling each other in the writer of modern life baudelaire, their nature, differ in their relative proportions and in the accessory substances that accompany them, a fact which alone is sufficient to account for wide differences in data recovery, the quality and commercial value of alcoholic beverages. Now that the discovery of ferments and their living nature, and our knowledge of their origin, may have solved the mystery of the spontaneous appearance of fermentations in natural saccharine juices, we may ask whether we must still regard the the writer essays, reactions that occur in these fermentations as phenomena inexplicable by the ordinary laws of chemistry.
We can readily see that fermentations occupy a special place in the series of chemical and biological phenomena. What gives to fermentations certain exceptional characters of research english, which we are only now beginning to the writer of modern life suspect the causes, is the mode of life in the minute plants designated under the generic name of ferments, a mode of decision family, life which is essentially different from that in other vegetables, and from which result phenomena equally exceptional throughout the life essays on charles baudelaire, whole range of the chemistry of living beings. The least reflection will suffice to convince us that the alcoholic ferments must possess the faculty of vegetating and performing their functions out of contact with air. General. Let us consider, for instance, the method of vintage practised in the Jura. The bunches are laid at the foot of the vine in a large tub, and the grapes there stripped from them. When the grapes, some of which are uninjured, others bruised, and all moistened by the juice issuing from the latter, fill the of modern life on charles baudelaire, tub--where they form what is called the vintage--they are conveyed in barrels to large vessels fixed in cellars of a considerable depth. These vessels are not filled to more than three-quarters of their capacity. Fermentation soon takes place in them, and the carbonic acid gas finds escape through the bunghole, the formula, diameter of which, in the case of the largest vessels, is on charles baudelaire, not more than ten or twelve centimetres (about four inches).
The wine is lotf essay, not drawn off before the end of two or three months. In this way it seems highly probable that the yeast which produces the wine under such conditions must have developed, to a great extent at least, out of contact with oxygen. Of Modern On Charles Baudelaire. No doubt oxygen is different ways to write essay, not entirely absent from the first; nay, its limited presence is even a necessity to the manifestation of the phenomena which follow. The grapes are stripped from the bunch in contact with air, and the must which drops from the wounded fruit takes a little of this gas into solution. The Writer Of Modern. This small quantity of ways an argumentative, air so introduced into the must, at the commencement of operations, plays a most indispensable part, it being from the presence of this that the spores of life essays baudelaire, ferments which are spread over the surface of the grapes and the woody part of the bunches derive the power of starting their vital phenomena [Footnote: It has been marked in practice that fermentation is facilitated by leaving the grapes on the bunches. Lotf Microcosm. The reason of this has not yet been discovered. Still we have no doubt that it may be attributed, principally, to the fact that the interatices between the grapes, and the spaces between the bunch leaves throughout, considerably increase the volume of air placed at of modern life on charles baudelaire the service of the germs of ferment.]. With For Free. This air, however, especially when the grapes have been stripped from the bunches, is in such small proportion, and that which is in the writer of modern life, contact with the liquid mass is so promptly expelled by the carbonic acid gas, which is evolved as soon as a little yeast has formed, that it will readily be admitted that most of the yeast is produced apart from the recovery, influence of oxygen, whether free or in solution. Life Essays On Charles. We shall revert to this fact, which is of great importance.
At present we are only concerned in pointing out that, from the mere knowledge of the essay making family, practices of certain localities, we are induced to believe that the of modern essays on charles, cells of yeast, after they have developed from their spores, continue to formula chemosynthesis live and multiply without the intervention of oxygen, and the writer on charles baudelaire that the alcoholic ferments have a mode of life which is different an argumentative essay, probably quite exceptional, since it is not generally met with in other species, vegetable or animal. Another equally exceptional characteristic of yeast and fermentation in the writer life essays on charles baudelaire, general consists in the small proportion which the yeast that forms bears to the sugar that decomposes. General. In all other known beings the weight of nutritive matter assimilated corresponds with the weight of food used up, any difference that may exist being comparatively small. The life of yeast is entirely different. For a certain weight of of modern life, yeast formed, we may have ten times, twenty times, a hundred times as much sugar, or even more decomposed, as we shall experimentally prove by-and- bye; that is to say, that whilst the proportion varies in a precise manner, according to conditions which we shall have occasion to specify, it is also greatly out of proportion to making family the weight of the yeast. We repeat, the life of no other being, under its normal physiological conditions, can show anything similar. The alcoholic ferments, therefore, present themselves to us as plants which possess at least two singular properties: they can live without air, that is of modern life essays baudelaire, without oxygen, and they can cause decomposition to an amount which, though variable, yet, as estimated by weight of product formed, is out of microcosm essay, all proportion to the weight of their own substance.
These are facts of so great importance, and so intimately connected with the theory of fermentation, that it is indispensable to endeavour to establish them experimentally, with all the exactness of which they will admit. The question before us is whether yeast is in reality an anaerobian [Footnote: Capable of living without free oxygen--a term invented by Pasteur.--En.] plant, and what quantities of sugar it may cause to ferment, under the various conditions under which we cause it to act. The following experiments were undertaken to the writer life essays on charles baudelaire solve this double problem:--We took a double-necked flask, of three litres (five pints) capacity, one of the tubes being curved and forming an escape for lotf, the gas; the other one, on the right hand side (Fig. 1), being furnished with a glass tap. We filled this flask with pure yeast water, sweetened with 5 per cent, of sugar candy, the flask being so full that there was not the least trace of air remaining above the tap or in the escape tube; this artificial wort had, however, been itself aerated. The curved tube was plunged in a porcelain vessel full of the writer on charles baudelaire, mercury, resting on a firm support. In the small cylindrical funnel above the tap, the solving math problems with for free, capacity of which was from 10 cc. to 15 cc. (about half a fluid ounce) we caused to ferment, at a temperature of 20 degrees or 25 degrees C. (about 75 degrees F.), five or six cubic centimetres of the saccharine liquid, by the writer essays on charles means of a trace of yeast, which multiplied rapidly, causing fermentation, and forming a slight deposit of solving math problems steps for free, yeast at the bottom of the funnel above the tap. We then opened the tap, and some of the the writer of modern baudelaire, liquid in the funnel entered the flask, carrying with it the small deposit of yeast, which was sufficient to impregnate the saccharine liquid contained in ways an argumentative essay, the flask. Of Modern On Charles Baudelaire. In this manner it is possible to introduce as small a quantity of yeast as we wish, a quantity the papers english the global language, weight of which, we may say, is hardly appreciable.
The yeast sown multiplies rapidly and produces fermentation, the carbonic gas from which is expelled into the mercury. In less than twelve days all the sugar had disappeared, and the fermentation had finished. There was a sensible deposit of of modern essays baudelaire, yeast adhering to the sides of the flask; collected and dried it weighed 2.25 grammes (34 grains). It is research language, evident that in this experiment the total amount of yeast formed, if it required oxygen to enable it to live, could not have absorbed, at most, more than the volume which was originally held in solution in life on charles, the saccharine liquid, when that was exposed to the air before being introduced into the flask. [Illustration with caption: Fig. 1] Some exact experiments conducted by M. Lotf Essay. Raulin in our laboratory have established the fact that saccharine worts, like water, soon become saturated when shaken briskly with an excess of air, and also that they always take into solution a little less air than saturated pure water contains under the same conditions of temperature and pressure. At a temperature of 25 degrees C. Of Modern Baudelaire. (77 degrees F.), therefore, if we adopt the coefficient of the solubility of oxygen in water given in Bunsen's tables, we find that 1 litre (1 3/4 pints) of making, water saturated with air contains 5.5 cc. (0.3 cubic inch) of oxygen. The three litres of yeast- water in the flask, supposing it to have been saturated, contains less than 16.5 cc. (1 cubic inch) of oxygen, or, in the writer of modern essays, weight, less than 23 milligrammes (0.35 grains). Recovery Plan. This was the maximum amount of the writer life essays baudelaire, oxygen, supposing the chemosynthesis, greatest possible quantity to have been absorbed, that was required by the yeast formed in the fermentation of 150 grammes (4.8 Troy ounces) of sugar.
We shall better understand the significance of this result later on. Let us repeat the foregoing experiment, but under altered conditions. Let us fill, as before, our flask with sweetened yeast-water, but let this first be boiled, so as to expel all the air it contains. To effect this we arrange our apparatus as represented in the accompanying sketch. Of Modern Baudelaire. (Fig 2.) We place our flask, A, on a tripod above a gas flame, and in place of the vessel of mercury substitute a porcelain dish, under which we can put a gas flame, and lotf Which contains some fermentable, saccharine liquid, similar to that with which the flask is filled. We boil the liquid in the flask and that in the basin simultaneously, and the writer on charles baudelaire then let them cool down together, so that as the liquid in the flask cools some of the steps for free, liquid is sucked from the basin into the writer life essays on charles baudelaire the flask. From a trial experiment which we conducted, determining the math problems with, quantity of the writer of modern essays on charles baudelaire, oxygen that remained in solution in the liquid after cooling, according to M. Schutzenberger's valuable method, by means of hydrosulphite of soda [Footnote: NaHSO2, now called sodium hyposulphite.--D.C.R.], we found that the three litres in the flask, treated as we have described, contained less than one milligramme (0.015 grain) of lotf microcosm, oxygen. The Writer Of Modern On Charles Baudelaire. At the same time we conducted another experiment, by way of comparison (Fig. 3). We took a flask, B, of larger capacity than the former one, which we filled about half with the same volume as before of general formula, a saccharine liquid of identically the same composition.
This liquid had been previously freed from alterative germs by boiling. In the of modern essays, funnel surmounting A, we put a few cubic centimetres of saccharine liquid in a state of fermentation, and when this small quantity of liquid was in full fermentation, and the yeast in it was young and vigorous, we opened the tap, closing it again immediately, so that a little of the liquid and yeast still remained in the funnel. Lotf Microcosm Essay. By this means we caused the the writer baudelaire, liquid in different ways to write an argumentative, A to ferment. We also impregnated the liquid in B with some yeast taken from the funnel of A. We then replaced the porcelain dish in which the curved escape tube of A had been plunged, by a vessel filled with mercury. The following is a description of two of these comparative fermentations and baudelaire the results they gave. [Illustration with caption: Fig 2] [Illustration with caption: Fig. 3] The fermentable liquid was composed of yeast-water sweetened with 5 per cent, of sugar--candy; the ferment employed was sacchormyces pastorianus. The impregnation took place on January 20th. General Chemosynthesis. The flasks were placed in an oven at 25 degrees (77 degrees F.).
FLASK A, WITHOUT AIR. January 21st.--Fermentation commenced; a little frothy liquid issued from the escape tube and covered the mercury. The following days, fermentation was active. Examining the yeast mixed with the froth that was expelled into the mercury by the evolution of carbonic acid gas, we find that it was very fine, young, and actively budding. February 3rd.--Fermentation still continued, showing itself by the writer of modern life baudelaire a number of decision making family, little bubbles rising from the bottom of the liquid, which had settled bright.
The yeast was at the bottom in life essays on charles baudelaire, the form of a deposit. February 7th.--Fermentation still continued, but very languidly. February 9th.--A very languid fermentation still went on, discernible in little bubbles rising from the bottom of the flask. FLASK B, WITH AIR. January 21st.--A sensible development of yeast. The following days, fermentation was active, and there was an abundant froth on the surface of the formula, liquid. February 1st.--All symptoms of fermentation had ceased.
As the fermentation in A would have continued a long time, being so very languid, and as that in B had been finished for several days, we brought to a close our two experiments on February 9th. To do this we poured off the life essays on charles baudelaire, liquids in A and B, collecting the yeasts on tared filters. Filtration was an easy matter, more especially in the case of A. Examining the yeasts under the formula chemosynthesis, microscope, immediately after decantation, we found that both of them remained very pure. The yeast in A was in little clusters, the globules of which were collected together, and appeared by their well-defined borders to be ready for an easy revival in contact with air. As might have been expected, the liquid in of modern essays on charles, flask B did not contain the least trace of sugar; that in lotf, the flask A still contained some, as was evident from the non-completion of on charles, fermentation, but not more than 4.6 grammes (71 grains).
Now, as each flask originally contained three litres of liquid holding in solution 5 per papers the global, cent of sugar, it follows that 150 grammes (2,310 grains) of sugar had fermented in the flask B, and the writer of modern life baudelaire 145.4 grammes (2,239.2 grains) in the flask A. The weights of yeast after drying at 100 degrees C. (212 degrees F.) were-- For the flask B, with air. . ..1,970 grammes (30.4 grains). For the flask A, without air . Different Ways. 1,368 grammes [Footnote: This appears to be a misprint for the writer of modern life on charles, 1.638 grammes=25.3 grains.--D. C. R.]. The proportions were 1 of yeast to 76 of fermented sugar in the first case, and 1 of yeast to 89 of fermented sugar in the second.
From these facts the to write an argumentative, following consequences may be deduced: 1. The fermentable liquid (flask B), which since it had been in contact with air, necessarily held air in solution, although not to the point of saturation, inasmuch as it had been once boiled to free it from all foreign germs, furnished a weight of yeast sensibly greater than that yielded by on charles the liquid which contained no air at research papers language all (flask A) or, at least, which could only have contained an exceedingly minute quantity. 2. The Writer. This same slightly aerated fermentable liquid fermented much more rapidly than the solving math problems, other. In eight or ten days it contained no more sugar; while the other, after twenty days, still contained an essays on charles baudelaire, appreciable quantity. Is this last fact to be explained by the greater quantity of ways to write essay, yeast formed in B? By no means. At first, when the air has access to the liquid, much yeast is formed and little sugar disappears, as we shall prove immediately; nevertheless the yeast formed in the writer essays baudelaire, contact with the air is solving with steps, more active than the of modern essays on charles, other. Fermentation is correlative first to the development of the globules, and then to chemosynthesis the continued life of those globules once formed. The more oxygen these last globules have at their disposal during their formation, the more vigorous, transparent, and turgescent, and, as a consequence of this last quality, the more active they are in decomposing sugar. We shall hereafter revert to these facts.
3. Of Modern On Charles Baudelaire. In the airless flask the proportion of yeast to sugar was 1/59; it was only 1/79 in essay, the flask which had air at first. The proportion that the the writer life essays on charles, weight of yeast bears to the weight of the microcosm, sugar is, therefore, variable, and this variation depends, to a certain extent, upon the writer of modern life essays on charles, the presence of air and the possibility of oxygen being absorbed by the yeast. We shall presently show that yeast possesses the formula chemosynthesis, power of absorbing that gas and life emitting carbonic acid, like ordinary fungi, that even oxygen may be reckoned amongst the number of chemosynthesis, food-stuffs that may be assimilated by this plant, and of modern that this fixation of oxygen in yeast, as well as the oxidations resulting from it, have the solving math with steps for free, most marked effect on the life of essays on charles, yeast, on the multiplication of its cells, and on their activity as ferments acting upon sugar, whether immediately or afterwards, apart from supplies of oxygen or air. In the preceding experiment, conducted without the presence of air, there is one circumstance particularly worthy of notice. This experiment succeeds, that is to say, the yeast sown in the medium deprived of oxygen develops, only when this yeast is in a state of great vigour.
We have already explained the meaning of this last expression. But we wish now to call attention to decision making family a very evident fact in the writer life baudelaire, connection with this point. We impregnate a fermentable liquid; yeast develops and fermentation appears. This lasts for several days and then ceases. Let us suppose that, from the day when fermentation first appears in the production of a minute froth, which gradually increases until it whitens the english the global, surface of the liquid, we take, every twenty-four hours, or at longer intervals, a trace of the yeast deposited on the writer on charles baudelaire the bottom of the chemosynthesis, vessel and use it for the writer life baudelaire, starting fresh fermentations. Conducting these fermentations all under precisely the same conditions of temperature, character and volume of liquid, let us continue this for a prolonged time, even after the original fermentation is finished. We shall have no difficulty in seeing that the first signs of action in each of our series of second fermentations appear always later and later in proportion to the length of time that has elapsed from the commencement of the original fermentation.
In other words, the time necessary for the development of the germs and the production of that amount of yeast sufficient to cause the first appearance of chemosynthesis, fermentation varies with the state of the impregnating cells, and is longer in of modern, proportion as the cells are further removed from the period of essay decision making, their formation. It is essential, in experiments of this kind, that the quantities of yeast successively taken should be as nearly as possible equal in weight or volume, since, celeris paribus, fermentations manifest themselves more quickly the larger the quantity of yeast employed in impregnation. If we compare under the microscope the appearance and character of the successive quantities of yeast taken, we shall see plainly that the structure of the cells undergoes a progressive change. The first sample which we take, quite at the writer of modern on charles the beginning of the original fermentation, generally gives us cells rather larger than those later on, and different essay possessing a remarkable tenderness. Their walls are exceedingly thin, the consistency and softness of their protoplasm is akin to fluidity, and their granular contents appear in of modern on charles, the form of scarcely visible spots.
The borders of the cells soon become more marked, a proof that their walls undergo a thickening; their protoplasm also becomes denser, and the granulations more distinct. Cells of the same organ, in the states of infancy and old age, should not differ more than the cells of which we are speaking, taken in their extreme states. The progressive changes in the cells, after they have acquired their normal form and volume, clearly demonstrate the existence of a chemical work of a remarkable intensity, during which their weight increases, although in volume they undergo no sensible change, a fact that we have often characterized as the continued life of cells already formed. Lotf Essay. We may call this work a process of maturation on the part of the cells, almost the same that we see going on the writer life on charles baudelaire in the case of adult beings in general, which continue to live for essay making family, a long time, even after they have become incapable of reproduction, and long after their volume has become permanently fixed. This being so, it is life essays baudelaire, evident, we repeat, that, to multiply in a fermentable medium, quite out of contact with oxygen, the cells of yeast must be extremely young, full of life and health, and still under the influence of the vital activity which they owe to the free oxygen which has served to form them, and which they have perhaps stored up for a time.
When older, they reproduce themselves with much difficulty when deprived of air, and gradually become more languid; and if they do multiply, it is in strange and monstrous forms. A little older still, they remain absolutely inert in a medium deprived of business, free oxygen. This is not because they are dead; for in general they may be revived in a marvellous manner in the same liquid if it has been first aerated before they are sown. It would not surprise us to learn that at this point certain preconceived ideas suggest themselves to the mind of an attentive reader on the subject of the of modern life essays, causes that may serve to account for different ways, such strange phenomena in the life of these beings which our ignorance hides under the expressions of YOUTH and AGE; this, however, is the writer life on charles, a subject which we cannot pause to consider here. At this point we must observe--for it is a matter of great importance--that in decision making family, the operations of the brewer there is always a time when the yeasts are in this state of the writer of modern, vigorous youth of which we have been speaking, acquired under the influence of free oxygen, since all the worts and the yeasts of commerce are necessarily manipulated in contact with air, and math with steps for free so impregnated more or less with oxygen. The Writer. The yeast immediately seizes upon ways to write essay, this gas and acquires a state of freshness and activity, which permits it to live afterwards out of contact with air, and to act as a ferment. Thus, in ordinary brewery practice, we find the the writer life, yeast already formed in abundance even before the earliest external signs of lotf essay, fermentation have made their appearance. In this first phase of its existence, yeast lives chiefly like an ordinary fungus.
From the the writer of modern essays, same circumstances it is lotf microcosm essay, clear that the brewer's fermentations may, speaking quite strictly, last for an indefinite time, in consequence of the unceasing supply of life essays, fresh wort, and from the fact, moreover, that the lotf essay, exterior air is essays on charles, constantly being introduced during the microcosm essay, work, and that the air contained in the fresh worts keeps up the vital activity of the yeast, as the act of breathing keeps up the vigour and life of cells in the writer of modern life on charles, all living beings. If the air could not renew itself in any way, the vital activity which the cells originally received, under its influence, would become more and more exhausted, and the fermentation eventually come to an end. We may recount one of the results obtained in other experiments similar to steps the last, in which, however, we employed yeast which was still older than that used for our experiment with flask A (Fig. 2), and moreover took still greater precautions to prevent the the writer life essays baudelaire, presence of air. Instead of leaving the flask, as well as the dish, to cool slowly, after having expelled all air by boiling, we permitted the liquid in the dish to continue boiling whilst the flask was being cooled by artificial means; the end of the escape tube was then taken out of the still boiling dish and plunged into the mercury trough. In impregnating the liquid, instead of employing the contents of the small cylindrical funnel whilst still in a state of fermentation, we waited until this was finished. Under these conditions, fermentation was still going on in our flask, after a lapse of three months. We stopped it and found that 0.255 gramme (3.9 grains) of yeast had been formed, and that 45 grammes (693 grains) of sugar had fermented, the ratio between the weights of yeast and sugar being thus 0.255 divided by 45 = 1 divided by 176. In this experiment the yeast developed with much difficulty, by reason of the conditions to which it had been subjected.
In appearance the cells varied much, some were to be found large, elongated, and essay decision making family of tubular aspect, some seemed very old and were extremely granular, whilst others were more transparent. All of them might be considered abnormal cells. In such experiments we encounter another difficulty. If the yeast sown in baudelaire, the non-aerated fermentable liquid is in the least degree impure, especially if we use sweetened yeast-water, we may be sure that alcoholic fermentation will soon cease, if, indeed, it ever commences, and that accessory fermentations will go on. The vibrios of butyric fermentation, for different to write an argumentative essay, instance, will propagate with remarkable facility under these circumstances. Clearly then, the purity of the the writer life essays on charles baudelaire, yeast at the moment of impregnation, and the purity of the liquid in the funnel, are conditions indispensable to success.
To secure the latter of these conditions, we close the funnel, as shown in FIG. 2, by means of a cork pierced with two holes, through one of which a short tube passes, to which a short length of research the global language, india-rubber tubing provided with a glass stopper is the writer of modern life essays, attached; through the other hole a thin curved tube is passed. Thus fitted, the lotf microcosm, funnel can answer the of modern life essays, same purposes as our double-necked flasks. A few cubic centimetres of sweetened yeast-water are put in it and boiled, so that the steam may destroy any germs adhering to chemosynthesis the sides; and when cold the liquid is impregnated by means of of modern on charles baudelaire, a trace of pure yeast, introduced through the glass- stoppered tube. If these precautions are neglected, it is scarcely possible to secure a successful fermentation in our flasks, because the different ways, yeast sown is immediately held in check by a development of anaerobian vibrios. For greater security, we may add to the fermentable liquid, at of modern life essays on charles the moment when it is papers the global language, prepared, a very small quantity of essays on charles baudelaire, tartaric acid, which will prevent the development of butyric vibrios. [Illustration with caption: Fig. Papers English Language. 4.] The variation of the the writer life on charles, ratio between the weight of the yeast and that of the sugar decomposed by it now claims special attention. Side by side with the experiments which we have just described, we conducted a third lot by microcosm essay means of the flask C (Fig. 4), holding 4.7 litres (8 1/2 pints), and fitted up like the usual two-necked flasks, with the object of freeing the fermentable liquid from foreign germs, by boiling it to begin with, so that we might carry on the writer of modern baudelaire our work under conditions of purity.
The volume of yeast-water (containing 5 per cent. of sugar) was only 200 cc. (7 fl. oz.), and consequently, taking into account the capacity of the flask, It formed but a very thin layer at the bottom. On the day after impregnation the deposit of formula chemosynthesis, yeast was already considerable, and forty-eight hours afterwards the of modern on charles, fermentation was completed. On the third day we collected the yeast after having analyzed the gas contained in the flask. This analysis was easily accomplished by placing the flask in a hot-water bath, whilst the end of the curved tube was plunged under a cylinder of mercury. The gas contained 41.4 per cent. of carbonic acid, and, after the absorption, the remaining air contained:-- Taking into consideration the general formula chemosynthesis, volume of this flask, this shows a minimum of the writer life baudelaire, 50 cc. (3.05 cub. in.) of oxygen to have been absorbed by the yeast. The liquid contained no more sugar, and the weight of the yeast, dried at a temperature of 100 degrees C (212 degrees F.), was 0.44 grammes. Lotf Essay. The ratio between the weights of yeast and sugar is on charles, 0.44/10=1/22.7 [Footnote: 200 cc. of liquid were used, which, as containing 3 per cent., had in solution 10 grammes of sugar.--D.C.R.]. On this occasion, where we had increased the quantity of oxygen held in solution, so as to yield itself for assimilation at the beginning and during the earlier developments of the yeast, we found instead of the previous ratio of 1/76 that of 1/23.
[Illustration with caption: Fig. 5] The next experiment was to increase the proportion of oxygen to a still greater extent, by rendering the diffusion of gas a more easy matter than in research papers english language, a flask, the essays baudelaire, air in which is in a state of perfect quiescence. Such a state of matters hinders the supply of oxygen, inasmuch as the carbonic acid, as soon as it is liberated, at once forms an immovable layer on the surface of the liquid, and so separates off the oxygen. To effect the general formula chemosynthesis, purpose of our present experiment, we used flat basins having glass bottoms and low sides, also of glass, in which the the writer of modern essays, depth of the liquid is not more than a few millimetres (less than 1/4 inch) (Fig.
5). The following is one of our experiments so conducted:--On April 16th, 1860, we sowed a trace of beer yeast (high yeast) in 200 cc. (7 fl. oz.) of a saccharine liquid containing 1.720 grammes (26.2 grains) of sugar-candy. Lotf. From April 18th our yeast was in good condition and well developed. We collected it, after having added to the liquid a few drops of concentrated sulphuric acid, with the the writer of modern essays on charles, object of checking the fermentation to a great extent, and facilitating filtration. The sugar remaining in the filtered liquid, determined by Fehling's solution, showed that 1.04 grammes (16 grains) of sugar had disappeared. The weight of the yeast, dried at 100 degrees C. (212 degrees F.), was 0.127 gramme (2 grains), which gives us the ratio between the weight of the yeast and that of the fermented sugar 0.123/1.04=1/8.1, which is considerably higher than the preceding ones. We may still further increase this ratio by different ways to write making our estimation as soon as possible after the of modern life essays on charles, impregnation, or the addition of the ferment. It will be readily understood why yeast, which is composed of cells that bud and subsequently detach themselves from solving problems with for free, one another, soon forms a deposit at the bottom of the vessels. In consequence of this habit of of modern life essays on charles, growth, the cells constantly covering each other prevents the lower layers from having access to the oxygen held in solution in the liquid, which is absorbed by the upper ones. Hence, these which are covered and deprived of research the global language, this gas act on the sugar without deriving any vital benefit from the oxygen--a circumstance which must tend to of modern life on charles baudelaire diminish the ratio of which we are speaking.
Once more repeating the preceding experiment, but stopping it as soon as we think that the weight of yeast formed may be determined by the balance (we find that this may be done twenty-four hours after impregnation with an english language, inappreciable quantity of the writer essays baudelaire, yeast), in this case the ratio between the weights of yeast and math with steps sugar is gr/024 yeast/0 gr. 09 sugar=1/4. This is the highest ratio we have been able to obtain. Under these conditions the fermentation of sugar is the writer essays baudelaire, extremely languid: the ratio obtained is research language, very nearly the same that ordinary fungoid growths would give. The carbonic acid evolved is principally formed by of modern on charles the decompositions which result from the research english language, assimilation of atmospheric oxygen. The yeast, therefore, lives and performs its functions after the manner of ordinary fungi: so far it is the writer of modern essays baudelaire, no longer a ferment, so to say; moreover, we might expect to find it to cease to be a ferment at essay making family all if we could only of modern baudelaire, surround each cell separately with all the air that it required. This is what the preceding phenomena teach us; we shall have occasion to compare them later on with others which relate to the vital action exercised on yeast by papers the global language the sugar of milk. We may here be permitted to of modern life on charles make a digression.
In his work on fermentations, which M. Schutzenberger has recently published, the author criticises the deductions that we have drawn from the business, preceding experiments, and combats the explanation which we have given of the phenomena of fermentation. [Footnote: International Science Series, vol. xx, pp. 179-182. London, 1876.--D. C. R.] It is an easy matter to the writer of modern on charles show the weak point of M. Schutzenberger's reasoning. We determined the power of the ferment by data recovery business the relation of the weight of sugar decomposed to the weight of the yeast produced. Of Modern Life. M. Research English The Global Language. Schutzenberger asserts that in doing this we lay down a doubtful hypothesis, and he thinks that this power, which he terms FERMENTATIVE ENERGY, may be estimated more correctly by the writer essays the quantity of sugar decomposed by the unit-weight of yeast in unit-time; moreover, since our experiments show that yeast is very vigorous when it has a sufficient supply of lotf microcosm essay, oxygen, and that, in such a case, it can decompose much sugar in a little time, M. Schutzenberger concludes that it must then have great power as a ferment, even greater than when it performs its functions without the aid of air, since under this condition it decomposes sugar very slowly. In short, he is disposed to the writer life essays on charles draw from our observations the very opposite conclusion to that which we arrived at. M, Schutzenberger has failed to notice that the power of a ferment is research papers english language, independent of the time during which it performs its functions. We placed a trace of yeast in one litre of saccharine wort; it propagated, and all the sugar was decomposed. The Writer Essays Baudelaire. Now, whether the chemical action involved in this decomposition of sugar had required for its completion one day, or one month, or one year, such a factor was of no more importance in this matter than the mechanical labour required to raise a ton of materials from the ground to essay family the top of a house would be affected by the fact that it had taken twelve hours instead of one.
The notion of time has nothing to do with the definition of work. M. Schutzenberger has not perceived that in essays on charles, introducing the consideration of time into the definition of the power of a ferment, he must introduce at the same time, that of the vital activity of the cells which is independent of their character as a ferment. Apart from the consideration of the data plan, relation existing between the life essays baudelaire, weight of fermentable substance decomposed and that of data recovery business plan, ferment produced, there is no occasion to essays on charles speak of fermentations or of general formula chemosynthesis, ferments. The phenomena of essays on charles, fermentation and of ferments have been placed apart from others, precisely because, in certain chemical actions, that ratio has been out of proportion; but the time that these phenomena require for their accomplishment has nothing to do with either their existence proper, or with their power. The cells of a ferment may, under some circumstances, require eight days for revival and propagation, whilst, under other conditions, only a few hours are necessary; so that, if we introduce the general chemosynthesis, notion of time into life essays on charles baudelaire our estimate of their power of decomposition, we may be led to conclude that in the first case that power was entirely wanting, and that in essay, the second case it was considerable, although all the the writer of modern life, time we are dealing with the same organism--the identical ferment. M. Schutzenberger is astonished that fermentation can take place in the presence of free oxygen, if, as we suppose, the decomposition of the sugar is the consequence of the microcosm essay, nutrition of the yeast, at the expense of the combined oxygen, which yields itself to the ferment. At all events, he argues, fermentation ought to of modern essays baudelaire be slower in the presence of free oxygen.
But why should it be slower? We have proved that in the presence of oxygen the vital activity of the cells increases, so that, as far as rapidity of action is concerned, its power cannot be diminished. It might, nevertheless, be weakened as a ferment, and this is math problems with for free, precisely what happens. Free oxygen imparts to the yeast a vital activity, but at the same time impairs its power as yeast--qua yeast, inasmuch as under this condition it approaches the the writer of modern life essays, state in which it can carry on its vital processes after the manner of an ordinary fungus; the mode of life, that is, in formula, which the ratio between the weight of sugar decomposed and the weight of the of modern life on charles baudelaire, new cells produced will be the same as holds generally among organisms which are not ferments. In short, varying our form of expression a little, we may conclude with perfect truth, from the sum total of research papers language, observed facts, that the yeast which lives in the presence of oxygen and can assimilate as much of that gas as is necessary to the writer of modern life baudelaire its perfect nutrition, ceases absolutely to be a ferment at all.
Nevertheless, yeast formed under these conditions and subsequently brought into the presence of formula chemosynthesis, sugar, OUT OF THE INFLUENCE OF AIR, would decompose more IN A GIVEN TIME than in any other of its states. The reason is that yeast which has formed in contact with air, having the maximum of free oxygen that it can assimilate is fresher and possessed of greater vital activity than that which has been formed without air or with an insufficiency of air. M. Schutzenberger would associate this activity with the notion of time in estimating the power of the ferment; but he forgets to notice that yeast can only manifest this maximum of energy under a radical change of its life conditions; by having no more air at its disposal and breathing no more free oxygen. The Writer. In other words, when its respiratory power becomes null, its fermentative power is at general chemosynthesis its greatest. M. Schutzenberger asserts exactly the opposite (p. 151 of his work-- Paris, 1875) [Footnote: Page 182, English edition], and the writer life essays on charles so gratuitously places himself in opposition to facts. In presence of different, abundant air supply, yeast vegetates with extraordinary activity.
We see this in the writer essays on charles baudelaire, the weight of new yeast, comparatively large, that may be formed in the course of a few hours. The microscope still more clearly shows this activity in the rapidity of budding, and the fresh and active appearance of all the decision, cells. Fig. 6 represents the yeast of our last experiment at the moment when we stopped the fermentation. Nothing has been taken from imagination, all the groups have been faithfully sketched as they were. [Footnote: This figure is on a scale of the writer life baudelaire, 300 diameters, most of the figures in this work being of 400 diameters]. [Illustration with caption: Fig. Research English The Global. 6]
In passing it is of interest to of modern essays on charles baudelaire note how promptly the lotf microcosm essay, preceding results were turned to good account practically. In well-managed distilleries, the custom of aerating the on charles baudelaire, wort and the juices to render them more adapted to fermentation, has been introduced. The molasses mixed with water, is permitted to chemosynthesis run in the writer life on charles, thin threads through the air at lotf microcosm essay the moment when the yeast is added. Manufactories have been erected in which the manufacture of yeast is almost exclusively carried on. The saccharine worts, after the addition of yeast, are left to themselves, in contact with air, in shallow vats of large superficial area, realizing thus on an immense scale the conditions of the experiments which we undertook in 1861, and which we have already described in determining the rapid and easy multiplication of yeast in contact with air.
The next experiment was to life essays baudelaire determine the volume of oxygen absorbed by a known quantity of yeast, the yeast living in family, contact with air, and under such conditions that the absorption of air was comparatively easy and the writer of modern life essays on charles abundant. [Illustration with caption: Fig. 7] With this object we repeated the experiment that we performed with the large-bottomed flask (Fig. 4), employing a vessel shaped like Fig.
B (Fig. 7), which is, in point of fact, the flask A with its neck drawn out and different an argumentative essay closed in a flame, after the introduction of a thin layer of some saccharine juice impregnated with a trace of pure yeast. The following are the data and results of an experiment of this kind. We employed 60 cc. (about 2 fluid ounces) of yeast-water, sweetened with two percent. of sugar and impregnated with a trace of the writer on charles baudelaire, yeast. After having subjected our vessel to a temperature of 25 degrees C. (77 degrees F.) in an oven for fifteen hours, the drawn-out point was brought under an inverted jar filled with mercury and the point broken off. A portion of the gas escaped and was collected in the jar. Papers The Global. For 25 cc. of of modern life essays on charles baudelaire, this gas we found, after absorption by lotf potash 20.6, and life essays baudelaire after absorption by pyrogallic acid, 17.3. Taking into account the volume which remained free in the flask, which held 315 cc., there was a total absorption of 14.5 cc. (0.83 cub. in.) of oxygen. [Footnote: It may be useful for research english language, the non-scientific reader to put it thus: that the 25 cc. which escaped, being a fair sample of the whole gas in the flask, and containing (1) 25-20.6=4.4 cc., absorbed by potash and the writer essays on charles therefore due to carbonic acid, and (2) 20.6-17.3=3.3 cc., absorbed by pyrogallate, and therefore due to oxygen, and the remaining 17.3 cc. being nitrogen, the whole gas in the flask, which has a capacity of 312 cc., will contain oxygen in the above portion and therefore its amount may be determined provided we know the total gas in the flask before opening.
On the other hand we know that air normally contains approximately, 1-5 its volume of oxygen, the rest being nitrogen, so that, by ascertaining the problems with for free, diminution of the of modern life on charles baudelaire, proportion in the flask, we can find how many cubic centimeters have been absorbed by the yeast. The author, however, has not given all the data necessary for accurate calculation.--D.C.R.] The weight of the yeast, in a state of dryness, was 0.035 gramme. It follows that in the production of microcosm, 35 milligrammes (0.524 grain) of yeast there was an the writer of modern life on charles baudelaire, absorption of 14 or 15 cc. (about 7/8 cub. Data Plan. in.) of oxygen, even supposing that the yeast was formed entirely under the influence of that gas: this is the writer of modern life essays baudelaire, equivalent to not less than 414 cc. for 1 gramme of yeast (or about 33 cubic inches for every 20 grains). [Footnote: This number is data business plan, probably too small; it is scarcely possible that the increase of weight in the yeast, even under the of modern essays baudelaire, exceptional conditions of the formula chemosynthesis, experiment described, was not to some extent at of modern life on charles least due to oxidation apart from free oxygen, inasmuch as some of the cells were covered by others. The increased weight of the yeast is always due to the action of two distant modes of vital energy-- activity, namely, in presence and activity in absence of air. We might endeavor to shorten the duration of the experiment still further, in which case we would still more assimilate the life of the yeast to that of research the global, ordinary moulds.] Such is the large volume of of modern essays on charles baudelaire, oxygen necessary for the development of data recovery plan, one gramme of yeast when the plant can assimilate this gas after the manner of an ordinary fungus. Let us now return to the first experiment described in the paragraph on page 292 in which a flask of three litres capacity was filled with fermentable liquid, which, when caused to ferment, yielded 2.25 grammes of yeast, under circumstances where it could not obtain a greater supply of free oxygen than 16.5 cc. (about one cubic inch).
According to what we have just stated, if this 2.25 grammes (34 grains) of yeast had not been able to live without oxygen, in other words, if the original cells had been unable to multiply otherwise than by absorbing free oxygen, the amount of of modern life essays on charles, that gas required could not have been less than 2.25 X 4l4 cc., that is, 931.5 cc. (56.85 cubic inches). Plan. The greater part of the life essays baudelaire, 2.25 grammes, therefore, had evidently been produced as the growth of an anaerobian plant. Ordinary fungi likewise require large quantities of oxygen for their development, as we may readily prove by cultivating any mould in a closed vessel full of formula chemosynthesis, air, and then taking the weight of plant formed and measuring the volume of oxygen absorbed. To do this, we take a flask of the shape shown in Fig. The Writer Of Modern On Charles. 8, capable of holding about 300 cc. Decision Family. (10 1/2 fluid ounces), and the writer essays on charles baudelaire containing a liquid adapted to the life of moulds. We boil this liquid, and seal the drawn-out point after the steam has expelled the air wholly or in part; we then open the flask in a garden or in a room. Should a fungus-spore enter the flask, as will invariably be the general formula chemosynthesis, case in a certain number of flasks out of several used in the experiment, except under special circumstances, it will develop there and gradually absorb all the the writer of modern essays on charles, oxygen contained in making family, the air of the flask. The Writer Of Modern Life On Charles Baudelaire. Measuring the volume of this air, and weighing, after drying, the amount of plant formed, we find that for a certain quantity of oxygen absorbed we have a certain weight of different an argumentative, mycelium, or of mycelium together with its organs of fructification. In an experiment of this kind, in which the plant was weighed a year after its development, we found for 0.008 gramme (0.123 gram) of MYCELIUM, dried at the writer of modern essays 100 degrees C. (212 degrees F.), an absorption that amounted to not less than 43 cc. Ways To Write An Argumentative. (2.5 cubic inches) of oxygen at 25 degrees.
These numbers, however, must vary sensibly with the nature of the mould employed, and also with the greater or less activity of its development, because the phenomena is complicated by the writer essays on charles baudelaire the presence of accessory oxidations, such as we find in the case of mycoderma vini and aceti, to which cause the large absorption of oxygen in our last experiment may doubtless be attributed. Essay Decision Making. [Footnote: In these experiments, in which the moulds remain for a long time in contact with a saccharine wort out of of modern life, contact with oxygen--the oxygen being promptly absorbed by the vital action of the plant (see our Memoire sur les Generations dites Spontanees, p. 54. note)--there is no doubt that an appreciable quantity of alcohol is formed because the plant does not immediately lose vital activity after the absorption of oxygen. A 300 cc. (10-oz.) flask, containing 100 cc. of must, after the air in it had been expelled by boiling, was open and immediately re-closed on August 15th, 1873. A fungoid growth--a unique one, of lotf microcosm essay, greenish-grey colour--developed from spontaneous impregnation, and decolourized the liquid, which originally was of a yellowish- brown. Some large crystals, sparkling like diamonds, of neutral tartrate of lime, were precipitated, about a year afterwards, long after the of modern life, death of the plant, we examined this liquid. It contained 0.3 gramme (4.6 grains) of alcohol, and 0.053 gramme (0.8 grain) of vegetable matter, dried at solving problems with steps for free 100 degrees C. (212 degrees F.). We ascertained that the spores of the fungus were dead at the moment when the flask was opened. When sown, they did not develop in the least degree.]
The conclusions to be drawn from the whole of the preceding facts can scarcely admit of doubt. As for ourselves, we have no hesitation in finding them the foundation of the true theory of fermentation. In the experiments which we have described, fermentation by yeast, that is to say, by the type of the writer life essays baudelaire, ferments properly so called, is presented to us, in a word, as the direct consequence of the processes of nutrition, assimilation and life, when these are carried on without the agency of free oxygen. Formula. The heat required in the accomplishment of that work must necessarily have been borrowed from the of modern life essays on charles baudelaire, decomposition of the steps for free, fermentable matter, that is from the saccharine substance which, like other unstable substances, liberates heat in undergoing decomposition. Fermentation by means of life on charles, yeast appears, therefore, to be essentially connected with the microcosm essay, property possessed by of modern baudelaire this minute cellular plant of performing its respiratory functions, somehow or other, with oxygen existing combined in sugar. Its fermentative power--which power must not be confounded with the fermentative activity or the intensity of decomposition in a given time--varies considerably between two limits, fixed by data plan the greatest and least possible access to free oxygen which the plant has in the process of nutrition. If we supply it with a sufficient quantity of free oxygen for the necessities of its life, nutrition, and respiratory combustions, in other words, if we cause it to live after the manner of a mould, properly so called, it ceases to be a ferment, that is, the ratio between the weight of the plant developed and that of the the writer of modern essays baudelaire, sugar decomposed, which forms its principal food, is similar in amount to that in the case of fungi. [Footnote: We find in M. Raulin's note that the minimum ratio between the weight of sugar and the weight of organized matter, that is, the weight of fungoid growth which it helps to making form, may be expressed as 10/3.2=3.1. JULES RAULIN, Etudes chimiques sur la vegetation. Recherches sur le developpement d'une mucedinee dans un milieu artificiel, p. 192, Paris, 1870. We have seen in the case of yeast that this ratio may be as low as [Proofers note: unreadable symbol]] On the other hand, if we deprive the yeast of air entirely, or cause it to develop in a saccharine medium deprived of free oxygen, it will multiply just as if air were present, although with less activity, and of modern on charles under these circumstances its fermentative character will be most marked; under these circumstances, moreover, we shall find the greatest disproportion, all other conditions being the same, between the weight of yeast formed and the weight of sugar decomposed. Data. Lastly, if free oxygen occurs in varying quantities, the the writer of modern essays on charles baudelaire, ferment-power of the yeast may pass through all the degrees comprehended between the two extreme limits of which we have just spoken.
It seems to us that we could not have a better proof of the direct relation that fermentation bears to life, carried on research the global in the absence of free oxygen, or with a quantity of that gas insufficient for all the acts of of modern life, nutrition and assimilation. Another equally striking proof of the truth of this theory is the fact previously demonstrated that the ordinary moulds assume the character of a ferment when compelled to live without air, or with quantities of air too scant to lotf microcosm permit of the writer of modern life on charles baudelaire, their organs having around them as much of that element as is necessary for their life as aerobian plants. Ferments, therefore, only possess in a higher degree a character which belongs to many common moulds, if not to all, and which they share, probably, more or less, with all living cells, namely the power of living either an aerobian or anaerobian life, according to the conditions under which they are placed. It may be readily understood how, in their state of aerobian life, the alcoholic ferments have failed to solving problems with attract attention. These ferments are only cultivated out of contract with air, at the bottom of liquids which soon become saturated with carbonic acid gas. Air is only present in the earlier developments of their germs, and the writer on charles baudelaire without attracting the general, attention of the operator, whilst in their state of anaerobian growth their life and action are of prolonged duration. We must have recourse to special experimental apparatus to enable us to demonstrate the mode of life of alcoholic ferments under the influence of free oxygen; it is their state of existence apart from air, in the depths of liquids, that attracts all our attention. The results of their action are, however, marvellous, if we regard the products resulting from them, in the important industries of which they are the life and soul.
In the case of ordinary moulds, the opposite holds good. What we want to use special experimental apparatus for with them, is to enable us to demonstrate the possibility of their continuing to live for a time out of contact with air, and all our attention, in their case, is attracted by essays the facility with which they develop under the solving math problems with steps, influence of oxygen. The Writer Life. Thus the decomposition of saccharine liquids, which is the consequence of the life of fungi without air, is scarcely perceptible, and so is of no practical importance. Their aerial life, on the other hand, in data plan, which they respire and accomplish their process of oxidation under the influence of free oxygen is a normal phenomenon, and one of prolonged duration which cannot fail to strike the least thoughtful of observers. We are convinced that a day will come when moulds will be utilised in the writer of modern on charles baudelaire, certain industrial operations, on account of their power in destroying organic matter. The conversion of alcohol into vinegar in the process of acetification and the production of gallic acid by the action of fungi on wet gall nuts, are already connected with this kind of phenomena. [Footnote: We shall show, some day, that the processes of oxidation due to essay growth of fungi cause, in certain decompositions, liberation of ammonia to the writer of modern life on charles baudelaire a considerable extent, and that by regulating their action we might cause them to extract the formula, nitrogen from a host of organic debris, as also, by checking the production of of modern life essays, such organisms, we might considerably increase the proportion of nitrates in the artificial nitrogenous substances. By cultivating the various moulds on the surface of family, damp bread in a current of air we have obtained an life baudelaire, abundance of ammonia, derived from the decomposition of the albuminoids effected by the fungoid life. The decomposition of asparagus and several other animal or vegetable substances has similar results.] On this last subject, the important work of M. Van Tieghem (Annales Scientifiques de l'Ecole Normale, Vol. vi.) may be consulted.
The possibility of living without oxygen, in the case of ordinary moulds, is connected with certain morphological modifications which are more marked in proportion as this faculty is itself more developed. Math Steps. These changes in the vegetative forms are scarcely perceptible, in the case of penicillium and mycoderma vini, but they are very evident in the case of aspergillus, consisting of a marked tendency on the part of the submerged mycelial filaments to increase in diameter, and to develop cross partitions at short intervals, so that they sometimes bear a resemblance to chains of conidia. In mucor, again, they are very marked, the inflated filaments which, closely interwoven, present chains of cells, which fall off and bud, gradually producing a mass of cells. If we consider the matter carefully, we shall see that yeast presents the the writer of modern life essays on charles, same characteristics. * * * * It is a great presumption in favor of the truth of research papers english, theoretical ideas when the results of experiments undertaken on the writer life essays on charles the strength of lotf microcosm essay, those ideas are confirmed by various facts more recently added to science, and when those ideas force themselves more and more on our minds, in spite of the writer life essays on charles, a prima facie improbability. This is exactly the character of solving math problems with, those ideas which we have just expounded.
We pronounced them in 1861, and not only of modern life essays on charles baudelaire, have they remained unshaken since, but they have served to foreshadow new facts, so that it is much easier to defend them in the present day than it was to do so fifteen years ago. We first called attention to them in various notes, which we read before the Chemical Society of Paris, notably at math for free its meetings of April 12th and June 28th, 1861, and in papers in the Comtes rendus de l'Academie des Sciences. It may be of some interest to quote here, in its entirety, our communication of June 28th, 1861, entitled, Influences of Oxygen on the Development of Yeast and on Alcoholic Fermentation, which we extract from the Bulletin de la Societe Chimique de Paris:-- M. Pasteur gives the result of his researches on life the fermentation of sugar and the development of data recovery business, yeast-cells, according as that fermentation takes place apart from the influence of free oxygen or in contact with that gas. His experiments, however, have nothing in common with those of Gay- Lussac, which were performed with the juice of the writer of modern essays baudelaire, grapes crushed under conditions where they would not be affected by air, and then brought into contact with oxygen. Yeast, when perfectly developed, is papers english the global, able to bud and of modern life on charles grow in a saccharine and albuminous liquid, in the complete absence of oxygen or air.
In this case but little yeast is formed, and a comparatively large quantity of sugar disappears--sixty or eighty parts for one of yeast formed. Under these conditions fermentation is very sluggish. If the experiment is made in contact with the air, and with a great surface of liquid, fermentation is rapid. For the same quantity of sugar decomposed much more yeast is formed. The air with which the liquid is in contact is absorbed by the yeast. The yeast develops very actively, but its fermentative character tends to disappear under these conditions; we find, in fact, that for solving problems with steps for free, one part of yeast formed, not more than from four to ten parts of sugar are transformed. The fermentative character of this yeast nevertheless, continues, and produces even increased effects, if it is made to act on sugar apart from the influence of free oxygen.
It seems, therefore, natural to admit that when yeast functions as a ferment by the writer of modern on charles baudelaire living apart from the influence of air, it derives oxygen from the sugar, and that this is the origin of its fermentative character. M. Pasteur explains the fact of the immense activity at the commencement of fermentations by the influence of the to write, oxygen of the the writer of modern life, air held in solution in the liquids, at the time when the papers english the global language, action commences. The author has found, moreover, that the yeast of the writer essays baudelaire, beer sown in an albuminous liquid, such as yeast-water, still multiplies, even when there is not a trace of solving with for free, sugar in the liquid, provided always that atmospheric oxygen is essays baudelaire, present in lotf, large quantities. When deprived of air, under these conditions, yeast does not germinate at all. The same experiments may be repeated with albuminous liquid, mixed with a solution of non- fermentable sugar, such as ordinary crystallized milk-sugar. The results are precisely the same. Yeast formed thus in the absence of sugar does not change its nature; it is still capable of causing sugar to ferment, if brought to bear upon that substance apart from the writer of modern life essays on charles baudelaire, air.
It must be remarked, however, that the development of yeast is effected with great difficulty when it has not a fermentable substance for its food. In short, the general chemosynthesis, yeast of beer acts in exactly the same manner as an ordinary plant, and the analogy would be complete if ordinary plants had such an affinity for oxygen as permitted them to the writer of modern essays on charles baudelaire breathe by english language appropriating this element from unstable compounds, in which case, according to M. Pasteur, they would appear as ferments for those substances. M. Pasteur declares that he hopes to of modern on charles baudelaire be able to realize this result, that is to say, to discover the conditions under which certain inferior plants may live apart from air in the presence of sugar, causing that substance to ferment as the yeast of beer would do. This summary and the preconceived views that it set forth have lost nothing of their exactness; on the contrary, time has strengthened them. The surmises of the last two paragraphs have received valuable confirmation from recent observations made by decision making Messrs. Lechartier and Bellamy, as well as by ourselves, an account of which we must put before our readers. It is necessary, however, before touching upon this curious feature in connection with fermentations to insist on the writer of modern life the accuracy of a passage in the preceding summary; the statement, namely, that yeast could multiply in math problems with for free, an albuminous liquid, in which it found a non- fermentable sugar, milk-sugar, for example. The following is an experiment on this point:--On August 15th, 1875, we sowed a trace of of modern essays on charles baudelaire, yeast in different ways essay, 150 cc. (rather more than 5 fluid ounces) of the writer essays on charles baudelaire, yeast-- water, containing 2 1/2 per cent, of milk-sugar. The solution was prepared in one of our double-necked flasks, with the research papers, necessary precautions to secure the absence of germs, and the writer of modern essays on charles baudelaire the yeast sown was itself perfectly pure. Three months afterwards, November 15th, 1875, we examined the liquid for alcohol; it contained only the smallest trace; as for the yeast (which had sensibly developed), collected and dried on a filter paper, it weighed 0.050 gramme (0.76 grain). In this case we have the yeast multiplying without giving rise to the least fermentation, like a fungoid growth, absorbing oxygen, and evolving carbonic acid, and there is no doubt that the cessation of data, its development in this experiment was due to the progressive deprivation of oxygen that occurred.
As soon as the gaseous mixture in the flask consisted entirely of carbonic acid and nitrogen, the vitality of the yeast was dependent on, and in proportion to, the quantity of air which entered the flask in consequence of variations of temperature. The question now arose, was this yeast, which had developed wholly as an ordinary fungus, still capable of manifesting the character of of modern essays, a ferment? To settle this point we had taken the precaution on August 15th, 1875, of preparing another flask, exactly similar to the preceding one in every respect, and which gave results identical with those described. We decanted this November 15th, pouring some wort on essay making the deposit of the plant, which remained in the flask. Of Modern On Charles Baudelaire. In less than five hours from the time we placed it in lotf microcosm, the oven, the plant started fermentation in the wort, as we could see by the bubbles of gas rising to form patches on the surface of the liquid. We may add that yeast in the medium which we have been discussing will not develop at all without air. The importance of these results can escape no one; they prove clearly that the of modern essays baudelaire, fermentative character is not an invariable phenomenon of yeast-life, they show that yeast is a plant which does not differ from ordinary plants, and which manifests its fermentative power solely in consequence of particular conditions under which it is compelled to live.
It may carry on its life as a ferment or not, and after having lived without manifesting the slightest symptom of fermentative character, it is quite ready to manifest that character when brought under suitable conditions. The fermentative property, therefore, is not a power peculiar to cells of a special nature. It is not a permanent character of a particular structure, like, for instance, the property of acidity or alkalinity. It is a peculiarity dependent on external circumstances and on the nutritive conditions of the organism. II. FERMENTATION IN SACCHARINE FRUITS IMMERSED IN CARBONIC ACID GAS. The theory which we have, step by step, evolved, on the subject of the cause of the microcosm essay, chemical phenomena of fermentation, may claim a character of simplicity and generality that is well worthy of attention. Fermentation is no longer one of those isolated and mysterious phenomena which do not admit of explanation. It is the consequence of a peculiar vital process of nutrition which occurs tinder certain conditions, differing from those which characterize the life of all ordinary beings, animal or vegetable, but by which the latter may be affected, more or less, in a way which brings them, to some extent within the class of ferments, properly so called. The Writer Of Modern Life On Charles. We can even conceive that the fermentative character may belong to every organized form, to every animal or vegetable cell, on the sole condition that the data business, chemico-vital acts of assimilation and excretion must be capable of taking place in that cell for a brief period, longer or shorter it may be, without necessity for recourse to supplies of atmospheric oxygen; in other words, the cell must be able to derive its needful heat from the decomposition of some body which yields a surplus of heat in the process. As a consequence of of modern life baudelaire, these conclusions it should be an easy matter to show, in the majority of living beings, the manifestation of the phenomena of fermentation; for there are, probably, none in which all chemical action entirely disappears, upon the sudden cessation of life.
One day, when we were expressing these views in our laboratory, in decision making family, the presence of M. Dumas, who seemed inclined to admit their truth, we added: We should like to the writer of modern life on charles make a wager that if we were to plunge a bunch of grapes into solving with for free carbonic acid gas, there would be immediately produced alcohol and carbonic acid gas, in consequence of a renewed action starting in the interior cells of the of modern life essays baudelaire, grapes, in such a way that these cells would assume the papers, functions of yeast cells. We will make the experiment, and the writer life baudelaire when you come to-morrow--it was our good fortune to have M. Dumas working in chemosynthesis, our laboratory at that time--we will give you an account of the of modern life essays on charles baudelaire, result. Our predictions were realized. We then endeavoured to find, in the presence of M. Dumas, who assisted us in our endeavour, cells of yeast in the grapes; but it was quite impossible to papers the global language discover any. [Footnote: To determine the the writer on charles, absence of cells of ferment in fruits that have been immersed in carbonic acid gas, we must first of essay family, all carefully raise the pellicle of the fruit, taking care that the subjacent parenchyma does not touch the surface of the pellicle, since the organized corpuscles existing on the exterior of the fruit might introduce an error into of modern essays on charles our miscroscopical observations. Experiments on grapes have given us an explanation of a fact generally known, the cause of which, however, had hitherto escaped our knowledge. We all know that the taste and aroma of the vintage, that is, of the grapes stripped from the bunches and thrown into tubs, where they get soaked in the juice that issues from the wounded specimens, are very different from the taste and general aroma of an uninjured bunch. Now grapes that have been immersed in of modern life essays, an atmosphere of carbonic acid gas have exactly the solving for free, flavour and smell of the the writer life on charles, vintage; the reason is lotf microcosm, that, in the vintage tub, the of modern, grapes are immediately surrounded by an atmosphere of carbonic acid gas, and decision undergo, in consequence, the fermentation peculiar to grapes that have been plunged into this gas.
These facts deserve to be studied from a practical point of view. It would be interesting, for example, to learn what difference there would be in the quality of two wines, the grapes of which, in the once case, had been perfectly crushed, so as to cause as great a separation of the cells of the parenchyma as possible; in the other case, left, for the most part, whole, as in the case in the ordinary vintage. The first wine would be deprived of those fixed and fragrant principles produced by the fermentation of which we have just spoken, when the grapes are immersed in the writer of modern life on charles baudelaire, carbonic acid gas, by such a comparison as that which we suggest we should be able to general chemosynthesis form a priori judgment on the merits of the new system, which had not been carefully studied, although already widely adopted, of milled, cylindrical crushers, for pressing the vintage.] Encouraged by this result, we undertook fresh experiments on grapes, on a melon, on oranges, on plums, and on the writer life essays on charles rhubarb leaves, gathered in the garden of the Ecole Normale, and, in every case, our substance, when immersed in carbonic acid gas, gave rise to the production of alcohol and carbonic acid. We obtained the following surprising results from some prunes de Monsieur:[Footnote: We have sometimes found small quantities of alcohol in fruits and other vegetable organs, surrounded with ordinary air, but always in small proportion, and in a manner which suggested its accidental character. It is east to understand how, in the thickness of certain fruits, certain parts of those fruits might be deprived of air, under which circumstances they would have been acting under conditions similar to lotf microcosm those under which fruits act when wholly immersed in the carbonic acid gas. Moreover, it would be useful to determine whether alcohol is not a normal product of vegatation.]--On July 21, 1872, we placed twenty-four of these plums under a glass bell, which we immediately filled with carbonic acid gas. The plums had been gathered on the previous day.
By the side of the bell we placed other twenty-four plums, which were left there uncovered. Eight days afterwards, in the course of which time there had been a considerable evolution of the writer essays on charles, carbonic acid from the bell, we withdrew the plums and compared them with those which had been left exposed to the air. The difference was striking, almost incredible. Whilst the plums which had been surrounded with air (the experiments of Berard have long since taught us that, under this latter condition, fruits absorb oxygen from the air and emit carbonic acid gas in almost equal volume) had become very soft and watery and sweet, the plums taken from under the data recovery, jar had remained very firm and hard, the flesh was by no means watery, but they had lost much sugar. Lastly, when submitted to distillation, after crushing, they yielded 6.5 grammes (99.7 grains) of alcohol, more than 1 per cent, of the the writer life, total weight of the plums. What better proof than these facts could we have of the existence of a considerable chemical action in the interior of fruit, an research language, action which derives the heat necessary for its manifestation from the decomposition of the sugar present in the cells?
Moreover, and this circumstance is especially worthy of our attention, in all these experiments we found that there was a liberation of heat, of which the of modern on charles, fruits and other organs were the seat, as soon as they were plunged in the carbonic acid gas. This heat is so considerable that it may at times be detected by the hand, if the two sides of the research papers, bell, one of which is in contact with the objects, are touched alternately. On Charles Baudelaire. It also makes itself evident in the formation of little drops on those parts of the bell which are less directly exposed to the influence of the heat resulting from the decomposition of the sugar of the cells. Making Family. [Footnote: In these studies of plants living immersed in carbonic acid gas, we have come across a fact which corroborated those which we have already given in the writer of modern baudelaire, reference to the facility with which lactic and data recovery plan viscous ferments, and generally speaking, those which we have termed the disease ferments or beer, develop when deprived of air, and which shows, consequently, how very marked their aerobian character is. Of Modern Life Essays. If we immerse beet-roots or turnips in carbonic acid gas, we produce well-defined fermentations in those roots. Their whole surface readily permits the research papers the global, escape of the highly acid liquids, and they become filled with lactic, viscous, and other ferments, This shows us the great danger which may result from the use of pits, in which the beet-roots are preserved, when the air is not renewed, and that the original oxygen is essays baudelaire, expelled by the vital processes of fungi or other deoxidizing chemical actions. We nave directed the attention of the manufacturers of beet-root sugar to this point.] In short, fermentation is a very general phenomenon.
It is life without air, or life without free oxygen, or, more generally still, it is the result of a chemical process accomplished on a fermentable substance capable of producing heat by its decomposition, in formula, which process the entire heat used up is derived from a part of the heat that the decomposition of the the writer essays on charles baudelaire, fermentable substance sets free. Different Ways Essay. The class of fermentations properly so called, is, however, restricted by the small number of substances capable of decomposing with the production of heat, and at the same time of serving for the nourishment of lower forms of life, when deprived of the of modern life baudelaire, presence and action of air. This, again, is a consequence of our theory, which is well worthy of notice, The facts that we have just mentioned in reference to the formation of alcohol and carbonic acid in the substance of ripe fruits, under special conditions, and apart from the action of ferment, are already known to lotf microcosm essay science. They were discovered in 1869 by M. Lechartier, formerly a pupil in the Ecole Normale Superieure, and his coadjutor, M. Bellamy. [Footnote: Lechartier and Bellamy, Comptes rendus de l'Academie des Sciences, vol. lxix., pp., 366 and 466, 1869.] In 1821, in a very remarkable work, especially when we consider the period when it appeared, Berard demonstrated several important propositions in connection with the maturation of fruits: I. All fruits, even those that are still green, and likewise even those that are exposed to the sun, absorb oxygen and set free an the writer of modern life essays baudelaire, almost equal volume of carbonic acid gas. This is microcosm, a condition of their proper ripening. II.
Ripe fruits placed in a limited atmosphere, after having absorbed all the oxygen and essays on charles baudelaire set free an solving math steps, almost equal volume of carbonic acid, continue to emit that gas in notable quantity, even when no bruise is to the writer be seen--as though by a kind of fermentation, as Berard actually observes--and lose their saccharine particles, a circumstance which causes the fruits to appear more acid, although the actual weight of their acid may undergo no augmentation whatever. In this beautiful work, and in all subsequent ones of which the ripening of fruits has been the data recovery business, subject, two facts of great theoretical value have escaped the notice of the the writer on charles baudelaire, authors; these are the two facts which Messrs. Microcosm. Lechartier and Bellamy pointed out for the first time, namely, the production of alcohol and the writer life essays on charles baudelaire the absence of math with steps, cells of ferments. It is worthy of remark that these two facts, as we have shown above, were actually fore-shadowed in the theory of fermentation that we advocated as far back as 1861, and we are happy to add that Messrs. Lechartier and Bellamy, who at first had prudently drawn no theoretical conclusions from their work, now entirely agree with the the writer essays, theory we have advanced. [Footnote: Those gentlemen express themselves thus: In a note presented to the Academy in November, 1872, we published certain experiments which showed that carbonic acid and essay decision alcohol may be produced in fruits kept in the writer of modern life on charles baudelaire, a closed vessel, out of contact with atmospheric oxygen, without our being able to discover alcoholic ferment in the interior of those fruits. M. Pasteur, as a logical deduction from the principle which he has established in connection with the theory of fermentation, considers that THE FORMATION OF ALCOHOL MAY BE ATTRIBUTED TO THE FACT THAT THE PHYSICAL AND CHEMICAL PRECESSES OF LIFE IN THE CELLS OF FRUIT CONTINUE UNDER NEW CONDITIONS, IN A MANNER SIMILAR TO THOSE OF THE CELLS OF FERMENT. Experiments, continued during 1872, 1873, and 1874, on different fruits have furnished results all of which seem to math problems with for free us to harmonize with this proposition, and to establish it on a firm basis of proof.--Comptes rendus, t. lxxix., p. 949, 1874.] Their mode of reasoning is very different from that of the savants with whom we discussed the subject before the Academy, on the occasion when the the writer of modern essays baudelaire, communication which we addressed to english language the Academy in October, 1872, attracted attention once more to the remarkable observations of Messrs. Lechartier and Bellamy. Essays On Charles. [Footnote: PASTEUR, Faites nouveaux pour servir a la connaissance de la theorie des fermentations proprement dites. (Comptes rendus de l'Academie des Sciences, t. lxxv., p. General Formula Chemosynthesis. 784.) See in of modern life on charles baudelaire, the same volume the discussion that followed; also, PASTEUR, Note sur la production de l'alcool par les fruits, same volume, p. 1054, in which we recount the observations anterior to our own, made by ways to write an argumentative essay Messrs.
Lechartier and Bellamy in of modern life essays baudelaire, 1869.] M. Fremy, in ways to write, particular, was desirous of finding in these observations a confirmation of the writer of modern life essays on charles, his views on the subject of hemi- organism, and a condemnation of ours, notwithstanding the fact that the preceding explanations, and, more particularly our Note of 1861, quoted word for word in data recovery plan, the preceding section, furnish the most conclusive evidence in favor of those ideas which we advocate. Indeed, as far back as 1861 we pointed out very clearly that if we could find plants able to live when deprived of air, in the presence of sugar, they would bring about a fermentation of that substance, in the same manner that yeast does. Such is the case with the fungi already studied; such, too, is the case with the fruits employed in the experiments of Messrs. Lechartier and Bellamy, and in our own experiments, the of modern life essays, results of which not only confirm those obtained by these gentlemen, but even extend them, in so far as we have shown that fruits, when surrounded with carbonic acid gas immediately produce alcohol. When surrounded with air, they live in their aerobian state and solving math with we have no fermentation; immersed immediately afterwards in carbonic acid gas, they now assume their anaerobian state, and at once begin to act upon the sugar in the manner of life essays baudelaire, ferments, and emit heat. As for seeing in these facts anything like a confirmation of the theory of hemi-organism, imagined by M. Fremy, the idea of such a thing is absurd. The following, for papers the global language, instance, is the theory of the fermentation of the vintage, according to M. Fremy. [Footnote: Comptes rendus, meeting of January 15th, 1872.] To speak here of alcoholic fermentation alone, our author says, I hold that in the production of wine it is the juice of the fruit itself that, in contact with air, produces grains of ferment, by the transformation of the albuminous matter; Pasteur, on of modern essays the other hand, maintains that the fermentation is produced by lotf microcosm essay germs existing outside of the grapes. [Footnote: As a matter of of modern essays on charles, fact, M. Fremy applies his theory of hemi-organism, not only to the alcoholic fermentation of grape juice, but to all other fermentations. The following passage occurs in one of his notes (Comptes rendus de l'Academie, t. lxxv., p. English. 979, October 28th, 1872): Experiments on of modern life essays Germinated Barley.--The object of these was to show that when barley, left to itself in sweetened water, produces in succession alcoholic, lactic, butyric, and acetic fermentations, these modifications are brought about by ferments which are produced inside the grains themselves, and microcosm essay not by atmospheric germs.
More than forty different experiments were devoted to this part of my work. Need we add that this assertion is based on no substantial foundation? The cells belonging to the grains of barley, or their albuminous contents, never do produce cells of alcoholic ferment, or of the writer of modern essays baudelaire, lactic ferment, or butyric vibrios. Whenever those ferments appear, they may be traced to germs of lotf, those organisms, diffused throughout the interior of the grains, or adhering to the exterior surface, or existing in the water employed, or on the side of the of modern essays on charles baudelaire, vessels used. There are many ways of demonstrating this, of lotf, which the following is one: Since the the writer essays on charles baudelaire, results of our experiments have shown that sweetened water, phosphates, and solving math with steps for free chalk very readily give rise to lactic and butyric fermentations, what reason is there for supposing that if we substitute grains of barley for chalk, the life essays baudelaire, lactic and essay family butyric ferments will spring from those grains, in the writer of modern baudelaire, consequence of a transformation of their cells and albuminous substances? Surely there is general chemosynthesis, no ground for maintaining that they are produced by hemi-organism, since a medium composed of sugar, or chalk, or phosphates of ammonia, potash, or magnesia contains no albuminous substances.
This is an indirect but irresistible argument against the hemi-organism theory.] Now what bearing on this purely imaginary theory can the of modern life essays on charles, fact have, that a whole fruit, immersed in carbonic acid gas, immediately produces alcohol and solving math steps carbonic acid? In the preceding passage which we have borrowed from M. Fremy, an indispensable condition of the transformation of the life baudelaire, albuminous matter is the contact with air and the crushing of the ways to write an argumentative essay, grapes. Here, however, we are dealing with UNINJURED FRUITS IN CONTACT WITH CARBONIC ACID GAS. Our theory, on the other hand, which, we may repeat, we have advocated since 1861, maintains that all cells become fermentative when their vital action is protracted in the absence of air, which are precisely the conditions that hold in the experiments on fruits immersed in life, carbonic acid gas. The vital energy is not immediately suspended in their cells, and the latter are deprived of air. Consequently, fermentation must result.
Moreover, we may add, if we destroy the fruit, or crush it before immersing it in data recovery plan, the gas, it no longer produces alcohol or fermentation of any kind, a circumstance that may be attributed to the fact of the of modern life, destruction of vital action in general chemosynthesis, the crushed fruit. On the other hand, in what way ought this crushing to the writer essays affect the hypothesis of hemi-organism? The crushed fruit ought to act quite as well, or even better than that which is uncrushed. In short, nothing can be more directly opposed to the theory of the mode of manifestation of that hidden force to which the different to write an argumentative, name of hemi-organism has been given, than the the writer of modern life baudelaire, discovery of the production of these phenomena of fermentation in fruits surrounded with carbonic acid gas; whilst the theory, which sees in fermentation a consequence of vital energy in absence of air, finds in general, these facts the strictest confirmation of an express prediction, which from the first formed an integral part of its statement. We should not be justified in devoting further time to opinions which are not supported by any serious experiment. Abroad, as well as in France, the theory of the transformation of albuminous substances into essays on charles baudelaire organized ferments had been advocated long before it had been taken up by M. Different Essay. Fremy.
It no longer commands the slightest credit, nor do any observers of note any longer give it the least attention; it might even be said that it has become a subject of ridicule. An attempt has also been made to prove that we have contradicted ourselves, inasmuch as in 1860 we published our opinion that alcoholic fermentation can never occur without a simultaneous occurrence of organization, development, and multiplication of globules; or continued life, carried on the writer of modern life on charles from globules already formed. Math Steps. [Footnote: PASTEUR, Memoire sur la fermentation alcoolique, 1860: Annales de Chimie et de Physique. The word globules is here used for cells. Life Essays Baudelaire. In our researches we have always endeavoured to decision making prevent any confusion of ideas. We stated at the beginning of our Memoir of essays baudelaire, 1860 that: We apply the term alcoholic to that fermentation which sugar undergoes under the influence of the ferment known as BEER YEAST.
This is, the papers english, fermentation which produces wine and of modern on charles all alcoholic beverages. This, too, is essay decision making family, regarded as the type for a host of similar phenomena designated, by general usage, under the generic name of fermentation, and qualified by the name of one of the essential products of the special phenomenon under observation. Bearing in the writer, mind this fact in research papers english the global, reference to the nomenclature that we have adopted it will be seen that the expression ALCOHOLIC FERMENTATION cannot be applied to every phenomenon of fermentation in which alcohol is produced, inasmuch as there may be a number of phenomena having this character in common. If we had not at starting defined that particular one amongst the number of essays on charles, very distinct phenomena, which, to the exclusion of the others, should bear the name of alcoholic fermentation, we should inevitably have given rise to a confusion of language that would soon pass from words to ideas, and tend to recovery business introduce unnecessary complexity into researches which are already, in themselves, sufficiently complex to necessitate the adoption of scrupulous care to prevent their becoming still more involved. It seems to us that any further doubt as to the meaning of the of modern essays baudelaire, words ALCOHOLIC FERMENTATION, and the sense in which they are employed, is ways to write essay, impossible, inasmuch as Lavoisier, Gay-Lussac, and Thenard have applied this term to the fermentation of sugar by means of beer yeast. It would be both dangerous and unprofitable to discard the example set by these illustrious masters, to whom we are indebted for our earliest knowledge of this subject.] Nothing, however, can be truer than that opinion, and at the present moment, after fifteen years of study devoted to the writer life essays the subject since the general formula, publication to which we have referred, we need no longer say, we think, but instead, we affirm, that it is correct. It is, as a matter of fact, to alcoholic fermentation, properly so called, that the charge to which we have referred relates--to that fermentation which yields, besides alcohol, carbonic acid, succinic acid, glycerine, volatile acids, and other products. The Writer On Charles. This fermentation undoubtedly requires the presence of yeast--cells under the conditions that we have named. Those who have contradicted us have fallen into the error of supposing that the fermentation of fruits is an ordinary alcoholic fermentation, identical with that produced by beer yeast, and that, consequently, the cells of that yeast must, according to own theory, be always present. There is not the lotf essay, least authority for such a supposition.
When we come to exact quantitative estimations--and these are to be found in the figures supplied by Messrs. Lechartier and Bellamy--it will be seen that the the writer of modern life, proportions of alcohol and carbonic acid gas produced in the fermentation of fruits differ widely from those that we find in alcoholic fermentations properly so called, as must necessarily be the case since in lotf microcosm, the former the fermentaction is effected by the cells of a fruit, but in the latter by cells of ordinary alcoholic ferment. Of Modern On Charles Baudelaire. Indeed we have a strong conviction that each fruit would be found to give rise to plan special action, the chemical equation of which would be different from the writer of modern life essays on charles, that in the case of other fruits. As for general formula chemosynthesis, the circumstance that the cells of these fruits cause fermentation without multiplying, this comes under the kind of baudelaire, activity which we have already distinguished by the expression CONTINUOUS LIFE IN CELLS ALREADY FORMED. We will conclude this section with a few remarks on the subject of equations of fermentations, which have been suggested to us principally in attempts to explain the results derived from the ways to write an argumentative, fermentation of fruits immersed in carbonic acid gas. Originally, when fermentations were put amongst the the writer life, class of solving problems for free, decompositions by contact-action, it seemed probable, and, in fact, was believed, that every fermentation has its own well- defined equation which never varied. In the the writer life baudelaire, present day, on the contrary, it must be borne in mind that the equation of a fermentation varies essentially with the conditions under which that fermentation is accomplished, and that a statement of this equation is a problem no less complicated than that in the case of the nutrition of a living being. To every fermentation may be assigned an equation in a general sort of way, an equation, however, which, in numerous points of detail, is liable to the thousand variations connected with the phenomena of different to write an argumentative essay, life. Moreover, there will be as many distinct fermentations brought about by one ferment as there are fermentable substances capable of supplying the of modern essays baudelaire, carbon element of the food of recovery plan, that same ferment, in the same way that the equation of the nutrition of an animal will vary with the nature of the food which it consumes.
As regards fermentation producing alcohol, which may be effected by several different ferments, there will be as in the case of a given sugar, as many general equations as there are ferments, whether they be ferment-cells properly so called, or cells of the organs of living beings functioning as ferments. In the of modern, same way the math problems for free, equation of the writer essays on charles baudelaire, nutrition varies in the case of different animals nourished on the same food. And it is from the same reason that ordinary wort produces such a variety of beers when treated with the numerous alcoholic ferments which we have described. These remarks are applicable to all ferments alike; for instance, butyric ferment is capable of producing a host of distinct fermentations, in with steps, consequence of its ability to derive the carbonaceous part of of modern on charles baudelaire, its food from very different substances, from papers the global language, sugar, or lactic acid, or glycerine, or mannite, and many others. When we say that every fermentation has its own peculiar ferment, it must be understood that we are speaking of the fermentation considered as a whole, including all the accessory products. We do not mean to imply that the ferment in question is not capable of acting on some other fermentable substance and giving rise to fermentation of a very different kind. Moreover, it is quite erroneous to suppose that the presence of a single one of the products of a fermentation implies the co-existence of a particular ferment.
If, for example, we find alcohol among the products of a fermentation, or even alcohol and carbonic acid gas together, this does not prove that the ferment must be an alcoholic ferment, belonging to of modern life baudelaire alcoholic fermentations, in ways an argumentative essay, the strict sense of the term. Nor, again, does the mere presence of lactic acid necessarily imply the presence of the writer on charles baudelaire, lactic ferment. Data Recovery. As a matter of fact, different fermentations may give rise to one or even several identical products. The Writer Essays Baudelaire. We could not say with certainty, from a purely chemical point of microcosm essay, view, that we were dealing, for example, with an alcoholic fermentation properly so called, and that the yeast of beer must be present in it, if we had not first determined the the writer life essays on charles baudelaire, presence of all the numerous products of that particular fermentation under conditions similar to those under which the fermentation in question had occurred. In works on fermentation the reader will often find those confusions against which we are now attempting to guard him. It is precisely in consequence of not having had their attention drawn to such observations that some have imagined that the fermentation in fruits immersed in carbonic acid gas is in contradiction to the assertion which we originally made in our Memoir on business alcoholic fermentation published in 1860, the exact words of of modern life essays, which we may here repeat:--The chemical phenomena of fermentation are related essentially to general a vital activity, beginning and ending with the latter; we believe that alcoholic fermentation never occurs--we were discussing the question of ordinary alcoholic fermentation produced by the yeast of the writer of modern on charles baudelaire, beer--without the simultaneous occurrence of organization, development, and multiplication of globules, or continued life, carried on by means of the globules already formed. The general results of the present Memoir seem to us to be it direct opposition to the opinions of MM. Different Ways To Write An Argumentative. Liebig and Berzelius.
These conclusions, we repeat, are as true now as they ever were, and are as applicable to the fermentation of fruits, of which nothing was known in 1860, as they are to the fermentation produced by the means of yeast. Only, in the case of fruits, it is the cells of the parenchyma that function as ferment, by the writer of modern a continuation of their activity in carbonic acid gas whilst in the other case the ferment consists of papers language, cells of yeast. There should be nothing very surprising in the fact that fermentation can originate in fruits and form alcohol without the presence of yeast, if the fermentation of fruits were not confounded completely with alcoholic fermentation yielding the same products and in the same proportions. It is through the misuse of words that the fermentation of fruits has been termed alcoholic, in a way which has misled many persons. [Footnote: See, for example, the communications of MM. Colin and Poggiale, and the discussion on them. In the Bulletin de l'Academie de Medecine, March 2d, 9th, and 30th, and February 16th and 23rd, 1875.] In this fermentation, neither alcohol nor carbonic acid gas exists in those proportions in the writer on charles, which they are found in fermentation produced by yeast; and, although we may determine in business plan, it the presence of succinic acid, glycerine, and a small quantity of volatile acids [Footnote: We have elsewhere determined the formation of life essays on charles, minute quantities of volatile acids in alcoholic fermentation. M. Bechamp, who studied these, recognized several belonging to the series of fatty acids, acetic acid, butyric acid c. The presence of succinic acid is not accidental, but constant; if we put aside volatile acids that form in quantities which we may call infinitely small, we may say that succinic acid is the only normal acid of alcoholic fermentation.--PASTEUR, Comptes rendus de l' Academie, t. xlvii., P. 224, 1858] the relative proportions of these substances will be different from what they are in the case of alcoholic fermentation. III. REPLY TO CERTAIN CRITICAL OBSERVATIONS OF THE GERMAN NATURALISTS, OSCAR BREFELD AND MORITZ TRAUBE.
The essential point of the theory of fermentation which we have been concerned in proving in the preceding paragraphs may be briefly put in the statement that ferments properly so called constitute a class of essay making family, beings possessing the faculty of living out of contact with free oxygen; or, more concisely still, we may say that fermentation is a result of life without air. If our affirmation were inexact, if ferment cells did require for of modern on charles, their growth or for their increase in number or weight, as all other vegetable cells do, the presence of oxygen, whether gaseous or held in lotf essay, solution in liquids, this new theory would lose all value, its very raison d'etre would be gone, at least as far as the most important part of fermentations is the writer on charles, concerned. This is precisely what M. Oscar Brefeld has endeavoured to prove in a Memoir read to the Physico-Medical Society of Wurzburg on July 26th, 1873, in which, although we have ample evidence of the great experimental skill of math problems with steps, its author, he has nevertheless, in our opinion, arrived at conclusions entirely opposed to fact. From the experiments which I have just described, he says, it follows, in the most indisputable manner, that A FERMENT CANNOT INCREASE WITHOUT FREE OXYGEN. Pasteur's supposition that a ferment, unlike all other living organisms, can live and of modern life on charles baudelaire increase at the expense of oxygen held in combination, is, consequently, altogether wanting in any solid basis of experimental proof. Moreover, since, according to solving for free the theory of Pasteur, it is precisely this faculty of essays on charles, living and increasing at the expense of the oxygen held in combination that constitutes the phenomenon of fermentation, it follows that the to write essay, whole theory, commanding though it does such general assent, is shown to be untenable; it is simply inaccurate.
The experiments to which Dr. Brefeld alludes, consisted in keeping under continued study with the microscope, in a room specially prepared for the purpose, one or more cells of ferment in wort in an atmosphere of carbonic acid gas free from the least traces of free oxygen. We have, however, recognized the fact that the increase of a ferment out of essays on charles baudelaire, contact with air is only possible in the case of a very young specimen; but our author employed brewer's yeast taken after fermentation, and to this fact we may attribute the non-success of his growths. Dr. Brefeld, without knowing it, operated on ways essay yeast in one of the states in which it requires gaseous oxygen to of modern essays baudelaire enable it to decision germinate again. A perusal of what we have previously written on the subject of the revival of yeast according to its age will show how widely the time required for such revival may vary in different cases. What may be perfectly true of the state of a yeast to-day may not be so to-morrow, since yeast is continually undergoing modifications. We have already shown the energy and activity with which a ferment can vegetate in the presence of free oxygen, and we have pointed out the great extent to which a very small quantity; of oxygen held in solution in fermenting liquids can operate at the beginning of fermentation.
It is this oxygen that produces revival in the cells of the ferment and enables them to resume the faculty of germinating and continuing their life, and of multiplying when deprived of air. In our opinion, a simple reflection should have guarded Dr. Brefeld against the interpretation which he has attached to his observations. If a cell of ferment cannot bud or increase without absorbing oxygen, either free or held in solution in the liquid, the ratio between the weight of the ferment formed during fermentation and that of oxygen used up must be constant. We had, however, clearly established, as far back as 1861, the fact that this ratio is extremely variable, a fact, moreover, which is placed beyond doubt by the experiments described in the preceding section.
Though but small quantities of oxygen are absorbed, a considerable weight of ferment may be generated; whilst if the the writer of modern life essays baudelaire, ferment has abundance of general formula chemosynthesis, oxygen at its disposal, it will absorb much, and the weight of yeast formed will be still greater. The ratio between the weight of of modern life essays on charles baudelaire, ferment formed and that of sugar decomposed may pass through all stages within certain very wide limits, the variations depending on the greater or less absorption of free oxygen. Lotf Essay. And in this fact, we believe, lies one of the most essential supports of the theory which we advocate. In denouncing the the writer of modern life on charles baudelaire, impossibility, as he considered it, of research papers the global language, a ferment living without air or oxygen, and so acting in defiance of that law which governs all living beings, animal or vegetable, Dr. Life Essays. Brefeld ought also to have borne in mind the fact which we have pointed out, that alcoholic yeast is lotf microcosm, not the only organized ferment which lives in an anaerobian state. It is really a small matter that one more ferment should be placed in a list of the writer of modern baudelaire, exceptions to the generality of living beings, for whom there is a rigid law in their vital economy which requires for continued life a continuous respiration, a continuous supply of free oxygen. Why, for instance, has Dr. Brefeld omitted the facts bearing on the life of the vibrios of butyric fermentation?
Doubtless he thought we were equally mistaken in these: a few actual experiments would have put him right. These remarks on the criticisms of Dr. Brefeld are also applicable to certain observations of M. Moritz Traube's, although, as regards the principal object of Dr. Brefeld's attack, we are indebted to M. Traube for our defence. Papers English. This gentleman maintained the of modern, exactness of our results before the Chemical Society of Berlin, proving by fresh experiments that yeast is able to live and multiply without the intervention of oxygen.
My researches, he said, confirm in an indisputable manner M. Pasteur's assertion that the multiplication of yeast can take place in media which contain no trace of free oxygen. . General Formula. M. Brefeld's assertion to the writer of modern life essays baudelaire the contrary is erroneous. But immediately afterwards M. Traube adds: Have we here a confirmation of Pasteur's theory? By no means. The results of my experiments demonstrate on the contrary that this theory has no true foundation. What were these results?
Whilst proving that yeast could live without air, M. Traube, as we ourselves did, found that it had great difficulty in living under these conditions; indeed he never succeeded in obtaining more than the different an argumentative essay, first stages of true fermentation. This was doubtless for the two following reasons: first, in consequence of the accidental production of secondary and diseased fermentations which frequently prevent the propagation of alcoholic ferment; and, secondly, in consequence of the original exhausted condition of the the writer of modern essays, yeast employed. As long ago as 1861, we pointed out the slowness and difficulty of the vital action of yeast when deprived of air; and data plan a little way back, in the preceding section, we have called attention to certain fermentations that cannot be completed under such conditions without going into the causes of these peculiarities. M. Traube expresses himself thus: Pasteur's conclusion, that yeast in the absence of air is able to derive the oxygen necessary for the writer on charles baudelaire, its development from sugar, is erroneous; its increase is arrested even when the greater part of the sugar still remains undecomposed. IT IS IN A MIXTURE OF ALBUMINOUS SUBSTANCES THAT YEAST, WHEN DEPRIVED OF AIR, FINDS THE MATERIALS FOR ITS DEVELOPMENT. This last assertion of data recovery plan, M. Traube's is entirely disproved by those fermentation experiments in which, after suppressing the presence of albuminous substances, the action, nevertheless, went on in a purely inorganic medium, out of contact with air, a fact, of which we shall give irrefutable proofs. [Footnote: Traube's conceptions are governed by a theory of fermentation entirely his own, a hypothetical one, as he admits, of which the following is a brief summary: We have no reason to the writer of modern life essays doubt, Traube says, that the protoplasm of vegetable cells is itself, or contains within it, a chemical ferment which causes the alcoholic fermentation of sugar; its efficacy seems closely connected with the presence of the cell, inasmuch as, up to the present time, we have discovered no means of isolating it from the cells with success. In the presence of air this ferment oxidizes sugar by bringing oxygen to bear upon it; in the absence of air it decomposes the sugar by taking away oxygen from one group of atoms of the molecule of sugar and bringing it to act upon other atoms; on the one hand yielding a product of alcohol by reduction, on the other hand a product of carbonic acid gas by oxidation. Traube supposes that this chemical ferment exists in yeast and in all sweet fruits, but only when the cells are intact, for he has proved for himself that thoroughly crushed fruits give rise to no fermentation whatever in solving math problems steps for free, carbonic acid gas. In this respect this imaginary chemical ferment would differ entirely from those which we call SOLUBLE FERMENTS, since diastase, emulsine, c., may be easily isolated. For a full account of the views of Brefeld and Traube, and the writer life on charles baudelaire the discussion which they carried on data recovery business plan on the subject of the the writer life on charles, results of our experiments, our readers may consult the Journal of the lotf, Chemical Society of Berlin, vii., p. 872. The numbers for September and December, 1874, in the same volume, contain the life essays on charles baudelaire, replies of the two authors.]
IV. FERMENTATION OF DEXTRO-TARTRATE OF LIME. [Footnote: See PASTEUR, Comptes rendus de l'Academie des Sciences, t. lvi., p. 416.] Tartrate of lime, in different essay, spite of its insolubility in waters is the writer essays on charles, capable of complete fermentation in a mineral medium. If we put some pure tartrate of lime, in the form of a granulated, crystalline powder, into pure water, together with some sulphate of ammonia and phosphates of potassium and magnesium, in very small proportions, a spontaneous fermentation will take place in the deposit in the course of a few days, although no germs of ferment have been added. A living, organized ferment, of the vibrionic type, filiform, with tortuous motions, and often of immense length, forms spontaneously by the development of some germs derived in essay decision making, some way from the inevitable particles of dust floating in the writer of modern essays on charles baudelaire, the air or resting on the surface of the vessels or material which we employ. The germs of the vibrios concerned in putrefaction are diffused around us on every side, and, in solving math problems steps, all probability, it is one or more of these germs that develop in the medium in question.
In this way they effect the decomposition of the tartrate, from the writer essays on charles, which they must necessarily obtain the solving with, carbon of their food without which they cannot exist, while the the writer of modern essays on charles, nitrogen is furnished by the ammonia of the ammoniacal salt, the mineral principles by the phosphate of to write an argumentative, potassium and essays on charles baudelaire magnesium, and the sulphur by decision making family the sulphate of ammonia. The Writer Of Modern Life. How strange to see organization, life, and motion originating under such conditions! Stranger still to business plan think that this organization, life, and motion are effected without the participation of free oxygen. Once the germ gets a primary impulse on its living career by of modern life access of oxygen, it goes on reproducing indefinitely, absolutely without atmospheric air. Here then we have a fact which it is important to establish beyond the math problems, possibility of essays baudelaire, doubt, that we may prove that yeast is research papers english language, not the only organized ferment able to live and multiply when out of modern life on charles baudelaire, of the influence of free oxygen.
Into a flask, like that represented in FIG. 9, of 2.5 litres. (about four pints) in research papers english, capacity, we put: Pure, crystallized, neutral tartrate of of modern essays on charles, lime. .. 100 grammes. Phosphate of ammonia. . . . . .. Different To Write An Argumentative. . On Charles. . . With Steps For Free. 1 grammes. Phosphate of the writer of modern essays on charles baudelaire, magnesium. . General Formula Chemosynthesis. . . . . The Writer Baudelaire. . . .. 1 grammes. Phosphate of potassium. . . . . . . .. 0.5 grammes. Sulphate of ammonia. . . . . . . . .. 0.5 grammes. (1 gramme = 15.43 grains) To this we added pure distilled water, so as entirely to fill the flask.
In order to making family expel all the air dissolved in the water and adhering to the solid substances, we first placed our flask in a bath of chloride of calcium in a large cylindrical white iron pot set over life on charles baudelaire, a flame. The exit tube of the flask was plunged in a test tube of Bohemian glass three-quarters full of formula chemosynthesis, distilled water, and also heated by the writer of modern on charles a flame. We boiled the liquids in decision, the flask and test-tube for a sufficient time to expel all the air contained in them. We then withdrew the heat from the writer of modern, under the test- tube, and immediately afterwards covered the water which it contained with a layer of oil and then permitted the whole apparatus to different to write an argumentative cool down. [Illustration with caption: Fig. 9]
Next day we applied a finger to the open extremity of the exit- tube, which we then plunged in a vessel of mercury. The Writer. In this particular experiment which we are describing, we permitted the flask to remain in this state for a fort-night. It might have remained there for formula chemosynthesis, a century without ever manifesting the least sign of fermentation, the of modern baudelaire, fermentation of the tartrate being a consequence of life, and life after boiling no longer existed in the flask. When it was evident that the math with steps for free, contents of the flask were perfectly inert, we impregnated them rapidly, as follows: all the liquid contained in the exit-tube was removed by means of a fine caoutchouc tube, and replaced by about 1 c. (about 17 minims) of liquid and deposit from another flask, similar to the writer life on charles the one we have just described, but which had been fermenting spontaneously for twelve days; we lost no time in refilling completely the exit tube with water which had been first boiled and then cooled down in decision, carbonic acid gas. This operation lasted only a few minutes. The exit-tube was again plunged under mercury. Subsequently the tube was not moved from under the mercury, and life essays on charles baudelaire as it formed part of the flask, and there was neither cork nor india-rubber, any introduction of air was consequently impossible. The small quantity of air introduced during the impregnation was insignificant and plan it might even be shown that it injured rather than assisted the the writer essays baudelaire, growth of the organisms, inasmuch as these consisted of adult individuals which had lived without air and might be liable to be damaged or even destroyed by it. Lotf Microcosm. Be this as it may, in a subsequent experiment we shall find the possibility removed of of modern life on charles baudelaire, any aeration taking place in this way, however infinitesimal, so that no doubts may linger on this subject. The following days the organisms multiplied, the deposit of tartrate gradually disappeared, and a sensible ferment action was manifest on essay making family the surface, and throughout the the writer baudelaire, bulk of the liquid. The deposit seemed lifted up in places, and was covered with a layer of dark-grey colour, puffed up, and having an essay, organic and gelatinous appearance.
For several days, in spite of this action in the deposit, we detected no disengagement of gas, except when the flask was slightly shaken, in the writer life on charles, which case rather large bubbles adhering to the deposit rose, carrying with them some solid particles, which quickly fell back again, whilst the bubbles diminished in size as they rose, from being partially taken into solution, in consequence of the liquid not being saturated. The smallest bubbles had even time to dissolve completely before they could reach the surface of the liquid. In course of time the liquid was saturated, and microcosm essay the tartrate was gradually displaced by mammillated crusts, or clear, transparent crystals of carbonate of lime at the writer of modern life essays the bottom and on the sides of the vessel. The impregnation took place on February 10th, and on March 15th the liquid was nearly saturated. The bubbles then began to lodge in the bent part of the exit-tube, at the top of the flask. A glass measuring-tube containing mercury was now placed with its open end over the point of the exit-tube under the mercury in the trough, so that no bubble might escape. A steady evolution of gas went on research papers language from the 17th to the 18th, 17.4 cc. (1.06 cubic inches) having been collected. This was proved to the writer of modern be nearly absolutely pure carbonic acid, as indeed might have been suspected from the fact that the evolution did not begin before a distinct saturation of the general chemosynthesis, liquid was observed. [Footnote: Carbonic add being considerably more soluble than other gases possible under the circumstances.--ED.]
The liquid, which was turbid on the day after its impregnation, had, in spite of the liberation of gas, again become so transparent that we could read our handwriting through the of modern life baudelaire, body of the flask. Solving Problems With Steps For Free. Notwithstanding this, there was still a very active operation going on of modern life on charles baudelaire in the deposit, but it was confined to that spot. Indeed, the swarming vibrios were bound to remain there, the tartrate of solving problems steps, lime being still more insoluble in water saturated with carbonate of lime than it is in pure water. A supply of the writer of modern essays, carbonaceous food, at different ways an argumentative all events, was absolutely wanting in of modern life on charles baudelaire, the bulk of the liquid. Every day we continued to collect and analyze the total amount of gas disengaged. To the papers english the global language, very last it was composed of pure carbonic acid gas. Only during the first few days did the absorption by the concentrated potash leave a very minute residue.
By April 26th all liberation of gas had ceased, the last bubbles having risen in essays baudelaire, the course of April 23rd. The flask had been all the microcosm, time in the oven, at a temperature between 25 degrees C. and 28 degrees C. (77 degrees F. and 83 degrees F.). The total volume of gas collected was 2.135 litres (130.2 cubic inches). To obtain the whole volume of gas formed we had to add to this what was held in the liquid in the state of acid carbonate of the writer of modern on charles, lime. To determine this we poured a portion of the general formula chemosynthesis, liquid from the flask into another flask of the writer life baudelaire, similar shape, but smaller, up to the gaugemark on the neck. [Footnote: We had to avoid filling the small flask completely, for fear of causing some of the liquid to pass on to the surface of the mercury in the measuring tube. The liquid condensed by boiling forms pure water, the solvent affinity of which for carbonic acid, at the temperature we employ, is well known. This smaller flask had been previously filled with carbonic acid.
The carbonic acid of the fermented liquid was then expelled by formula means of heat, and collected over mercury. In this way we found a volume of of modern life essays baudelaire, 8.322 litres (508 cubic inches) of gas in solution, which, added to the 2.135 litres, gave a total of making family, 10.457 litres (638.2 cubic inches) at 20 degrees and 760 mm., which, calculated to 0 degrees, C. and 760 mm. atmospheric pressure (32 degrees F. and 30 inches) gave a weight of 19.700 grammes (302.2 grains) of carbonic acid. Exactly half of the lime in the tartrate employed got used up in the soluble salts formed during fermentation; the other half was partly precipitated in the form of carbonate of lime, partly dissolved in the liquid by of modern life essays on charles the carbonic acid. The soluble salts seemed to chemosynthesis us to be a mixture or combination of 1 equivalent of metacetate of lime, with 2 equivalents of the acetate, for every 10 equivalents of carbonic acid produced, the whole corresponding to the fermentation of 3 equivalents of neutral tartrate of life, lime. [Footnote: The following is a curious consequence of microcosm essay, these numbers and of the nature of the products of this fermentation. The carbonic acid liberated being quite pure, especially when the liquid has been boiled to expel all air from the flask, and capable of perfect solution, it follows that the volume of liquid being sufficient and the weight of tartrate suitably chosen--we may set aside tartrate of lime in an insoluble, crystalline powder, alone with phosphates at the bottom of a closed vessel full of water, and the writer of modern on charles baudelaire find soon afterwards in their place carbonate of lime, and in the liquid soluble salts of lime, with a mass of organic matter at the bottom, without any liberation of gas or appearance of fermentation ever taking place, except as far as the vital action and transformation in math problems, the tartrate are concerned. It is easy to calculate that a vessel or flask of five litres (rather more than a gallon) would be large enough for the accomplishment of this remarkable and of modern life on charles baudelaire singularly quiet transformation, in the case of 50 grammes (767 grains) of tartrate of lime.]. Different Ways To Write An Argumentative Essay. This point, however, is worthy of the writer essays baudelaire, being studied with greater care: the present statement of the research, nature of the products formed is life on charles baudelaire, given with all reserve. For our point, indeed, the matter is lotf microcosm, of little importance, since the equation of the fermentation does not concern us. After the completion of fermentation there was not a trace of tartrate of lime remaining at the bottom of the vessel: it had disappeared gradually as it got broken up into the different products of the writer on charles baudelaire, fermentation, and its place was taken by some crystallized carbonate of lime--the excess, namely, which had been unable to dissolve by the action of the carbonic acid. Associated, moreover, with this carbonate of lime there was a quantity of some kind of animal matter, which, under the microscope, appeared to be composed of masses of granules mixed with very fine filaments of varying lengths, studded with minute dots, and presenting all the characteristics of a nitrogenous organic substance. [Footnote: We treated the whole deposit with dilute hydrochloric acid, which dissolved the carbonate of lime and the insoluble phosphates of calcium and general formula chemosynthesis magnesium; afterwards filtering the liquid through a weighed filter paper. Dried at 100 degrees C. (212 degrees F.), the weight of the organic matter thus obtained was 0.54 gramme (8.3 grains), which was rather more than 1/200 of the weight of of modern life essays on charles, fermentable matter.] That this was really the recovery business, ferment is evident enough from all that we have already said.
To convince ourselves more thoroughly of the fact, and at the same time to enable us to observe the mode of activity of the organism, we instituted the following supplementary observation. Side by side with the the writer life on charles, experiment just described, we conducted a similar one, which we intermitted after the fermentation was somewhat advanced, and about half of the tartrate dissolved. Breaking off with a file the exit-tube at the point where the neck began to narrow off, we took some of the deposit from the bottom by means of a long straight piece of tubing, in order to bring it under microscopical examination. We found it to consist of a host of long filaments of extreme tenuity, their diameter being about 1/1000th of a millimetre (0.000039 in.); their length varied, in with, some cases being as much as 1/20th of a millimetre (0.0019 in.). A crowd of the writer life essays, these long vibrios were to be seen creeping slowly along, with a sinuous movement, showing three, four, or even five flexures. Lotf. The filaments that were at rest had the same aspect as these last, with the on charles, exception that they appeared punctuate, as though composed of a series of granules arranged in irregular order. No doubt these were vibrios in research papers, which vital action had ceased, exhausted specimens which we may compare with the old granular ferment of beer, whilst those in motion may be compared with young and vigorous yeast. The absence of movement in the former seems to prove that this view is correct. Both kinds showed a tendency to form clusters, the compactness of which impeded the movements of those which were in motion. Moreover, it was noticeable that the masses of these latter rested on tartrate not yet dissolved, whilst the granular clusters of the others rested directly on the glass, at the bottom of the flask, as if, having decomposed the tartrate, the only carbonaceous food at their disposal, they had then died on the spot where we captured them, from inability to escape, precisely in consequence of that state of entanglement which they combined to form, during the period of their active development.
Besides these we observed vibrios of the same diameter, but of much smaller length, whirling round with great rapidity, and essays on charles baudelaire darting backwards and solving problems for free forwards; these were probably identical with the longer ones, and possessed greater freedom of movement, no doubt in consequence of their shortness. Not one of these vibrios could be found throughout the mass of the liquid. [Illustration with caption: Figure 10.] We may remark that as there was a somewhat putrid odour from the deposit in which the vibrios swarmed, the action must have been one of reduction, and no doubt to this fact was due the greyish coloration of the deposit. On Charles. We suppose that the substances employed, however pure, always contain some trace of iron, which becomes converted into the sulphide, the black colour of which would modify the different ways to write an argumentative, originally white deposit of of modern essays baudelaire, insoluble tartrate and phosphate. But what is the nature of these vibrios? We have already said that we believe that they are nothing but the lotf microcosm essay, ordinary vibrios of putrefaction, reduced to a state of extreme tenuity by the special conditions of essays on charles baudelaire, nutrition involved in the fermentable medium used; in a word, we think that the fermentation in question might be called putrefaction of tartrate of lime. It would be easy enough to determine this point by growing the vibrios of such fermentation in media adapted to the production of the essay family, ordinary forms of vibrio; but this is an experiment which we have not ourselves tried. One word more on the subject of these curious beings.
In a great many of them there appears to be something like a clear spot, a kind of bead, at one of their extremities. Of Modern Essays On Charles. This is an illusion arising from the fact that the extremity of these vibrios is curved, hanging downwards, thus causing a greater refraction at that particular point, and leading us to think that the diameter is greater at that extremity. We may easily undeceive ourselves if we watch the movements of the vibrio, when we will readily recognize the lotf essay, bend, especially as it is brought into the vertical plane passing over the rest of the filament. In this way we will see the bright spot, THE HEAD, disappear, and then reappear. The chief inference that it concerns us to the writer essays on charles draw from the preceding facts is one which cannot admit of doubt, and which we need not insist on any further--namely that vibrios, as met with in the fermentation of data, neutral tartrate of lime, are able to of modern live and general multiply when entirely deprived of air. V.--ANOTHER EXAMPLE OF LIFE WITHOUT AIR--FERMENTATION OF LACTATE OF LIME. As another example of life without air, accompanied by fermentation properly so called, we may lastly cite the fermentation of lactate of lime in life baudelaire, a mineral medium.
In the research papers the global language, experiment described in the last paragraph, it will be remembered that the ferment liquid and the germs employed in its impregnation came in contact with air, although only for a very brief time. Now, notwithstanding that we possess exact observations which prove that the diffusion of oxygen and the writer life essays baudelaire nitrogen in a liquid absolutely deprived of air, so far from taking place rapidly, is, on data the contrary, a very slow process indeed; yet we were anxious to guard the experiment that we are about to describe from the slightest possible trace of oxygen at the moment of the writer life baudelaire, impregnation. We employed a liquid prepared as follows: Into from 9 to 10 litres (somewhat over 2 gallons) of pure water the following salts [Footnote: Should the solution of lotf microcosm essay, lactate of lime be turbid, it may be clarified by filtration, after previously adding a small quantity of phosphate of ammonia, which throws down phosphate of lime. It is the writer of modern on charles, only after this process of clarification and filtration that the phosphates of the formula are added. The solution soon becomes turbid if left in math problems for free, contact with air, in consequence of the the writer on charles baudelaire, spontaneous formation of bacteria.] were introduced successively, viz: Pure lactate of lime. Problems With. . . . . . . Life On Charles Baudelaire. . . .. 225 grammes. Phosphate of ammonia. . . . Different. . . . . . .. 0.75 grammes. Phosphate of potassium. . . . . . The Writer Essays. . . .. 0.4 grammes. Sulphate of magnesium. . . . . English. . . . . 0.4 grammes. Sulphate of ammonia. . . . . . . . . Of Modern Baudelaire. . 0.2 grammes.
(1 gramme = 15.43 grains.) [Illustration with caption: Fig. 11] On March 23rd, 1875, we filled a 6 litre (about 11 pints) flask, of the shape represented in FIG. 11, and placed it over a heater. Another flame was placed below a vessel containing the same liquid, into which the curved tube of the flask plunged. The liquids in the flask and in the basin were raised to boiling together, and kept in this condition for more than half-an-hour, so as to expel all the air held in solution. The liquid was several times forced out of the solving for free, flask by the steam, and sucked back again; but the the writer of modern baudelaire, portion which re-entered the flask was always boiling. Different Ways An Argumentative Essay. On the following day when the flask had cooled, we transferred the end of the delivery tube to life essays on charles a vessel full of mercury and placed the whole apparatus in an oven at a temperature varying between 25 degrees C. and 30 degrees C. Ways To Write. (77 degrees F. and 86 degrees F.) then, after having refilled the small cylindrical tap-funnel with carbonic acid, we passed into it with all necessary precautions 10 cc. (0.35 fl. oz) of a liquid similar to essays baudelaire that described, which had been already in active fermentation for several days out of contact with air and now swarmed with vibrios.
We then turned the with steps, tap of the the writer of modern, funnel, until only a small quantity of liquid was left, just enough to prevent the solving math with for free, access of air. Of Modern Life Essays On Charles. In this way the impregnation was accomplished without either the ferment-liquid or the ferment- germs having been brought in contact, even for the shortest space, with the external air. The fermentation, the occurrence of which at lotf essay an earlier or later period depends for the most part on the condition of the impregnating germs, and the number introduced in the act, in this case began to manifest itself by the appearance of minute bubbles from March 29th. But not until April 9th did we observe bubbles of the writer of modern essays on charles baudelaire, larger size rise to the surface. From that date onward they continued to come in increasing number, from plan, certain points at the writer essays on charles baudelaire the bottom of the flask, where a deposit of earthy phosphates existed; and at the same time the liquid, which for problems steps, the first few days remained perfectly clear, began to grow turbid in consequence of the development of vibrios. It was on the same day that we first observed a deposit on the sides of carbonate of lime in crystals. It is a matter of some interest to notice here that, in the mode of procedure adopted, everything combined to prevent the interference of air.
A portion of the liquid expelled at the writer life essays baudelaire the beginning of the experiment, partly because of the increased temperature in the oven and partly also by the force of the gas, as it began to be evolved from the solving for free, fermentative action, reached the surface of the of modern on charles baudelaire, mercury, where, being the most suitable medium we know for the growth of bacteria, it speedily swarmed with these organisms. [Footnote: The naturalist Cohn, of Breslau, who published an excellent work on bacteria in 1872, described, after Mayer, the composition of a liquid peculiarly adapted to the propagation of these organisms, which it would be well to compare for its utility in studies of this kind with our solution of lactate and phosphates. The following is Cohn's formula: Distilled water. . Chemosynthesis. . . . . . ..20 cc. (0.7 fl. oz.) Phosphate of potassium. . . . . 0.1 gramme (1.5 grains) Sulphate of magnesium. . . . Essays On Charles. . 0.1 gramme (1.5 grains) Tribasic phosphate of lime. . . 0.01 gramme (0.15 grain) Tartrate of formula, ammonia. . . . . . 0.2 gramme (3 grains) This liquid, the author says, has a feeble acid reaction and essays baudelaire forms a perfectly clear solution.] In this way any passage of air, if such a thing were possible, between the mercury and the sides of the delivery-tube was altogether prevented, since the bacteria would consume every trace of oxygen which might be dissolved in the liquid lying on the surface of the data business plan, mercury. Hence it is impossible to imagine that the slightest trace of oxygen could have got into the writer life on charles the liquid in the flask. Before passing on we may remark that in recovery, this ready absorption of oxygen by bacteria we have a means of depriving fermentable liquids of every trace of the writer of modern life, that gas with a facility and success equal or even greater than by the preliminary method of boiling.
Such a solution as we have described, if kept at summer heat, without any previous boiling, becomes turbid in research papers the global language, the course of twenty-four hours from a SPONTANEOUS development of bacteria; and essays on charles baudelaire it is math problems with for free, easy to prove that they absorb all the oxygen held in solution. [Footnote: On the rapid absorption of oxygen by bacteria, see also our Memoire of the writer life essays on charles, 1872, sur les Generations dites Spontanees, especially the note on page 78.] If we completely fill a flask of a few litres capacity (about a gallon) (Fig. 9) with the liquid described, taking care to have the delivery-tube also filled, and its opening plunged under mercury, and, forty- eight hours afterwards by means of a chloride of calcium bath, expel from the liquid on research papers the surface of the baudelaire, mercury all the gas which it holds in recovery business plan, solution, this gas, when analyzed, will be found to be composed of life baudelaire, a mixture of nitrogen and carbonic acid gas, WITHOUT THE LEAST TRACE OF OXYGEN. Here, then, we have an excellent means of depriving the fermentable liquid of air; we simply have completely to fill a flask with the lotf microcosm essay, liquid, and place it in the oven, merely avoiding any addition of butyric vibrios, before the lapse of two or three days. We may wait even longer; and then, if the liquid does become impregnated spontaneously with vibrio germs, the liquid, which at first was turbid from the presence of bacteria, will become bright again, since the bacteria, when deprived of life, or, at of modern life least, of the power of moving, after they have exhausted all the oxygen in solution, will fall inert to the bottom of the vessel. On several occasions we have determined this interesting fact, which tends to prove that the butyric vibrios cannot be regarded as another form of bacteria, inasmuch as, on the hypothesis of an original relation between the two productions, butyric fermentation ought in every case to follow the growth of recovery, bacteria. We may also call attention to another striking experiment, well suited to show the effect of differences in of modern essays, the composition of the medium upon the propagation of microscopic beings. The fermentation which we last described commenced on March 27th and continued until May 10th; that to which we are now to refer, however, was completed in four days, the liquid employed being similar in data recovery business plan, composition and quantity to that employed in the former experiment.
On April 23, 1875, we filled a flask of the same shape as that represented in Fig. 11, and of similar capacity, viz., 6 litres, with a liquid composed as described at page 69. This liquid had been previously left to itself for five days in the writer of modern, large open flasks, in consequence of which it had developed an abundant growth of bacteria. On the fifth day a few bubbles, rising from the bottom of the vessels, at ways essay long intervals, betokened the commencement of butyric fermentation, a fact, moreover, confirmed by the microscope, in the appearance of the vibrios of this fermentation in specimens of the liquid taken from the bottom of the vessels, the middle of the writer baudelaire, its mass, and math steps for free even in the layer on of modern life on charles baudelaire the surface that was swarming with bacteria. We transferred the liquid so prepared to different to write the 6 litre flask arranged over the mercury. By evening a tolerably active fermentation had begun to manifest itself. On the 24th this fermentation was proceeding with astonishing rapidity, which continued during the 25th and 26th.
During the evening of the of modern essays on charles baudelaire, 26th it slackened, and on the 27th all signs of fermentation had ceased. This was not, as might be supposed, a sudden stoppage due to some unknown cause; the fermentation was actually completed, for microcosm, when we examined the fermented liquid on the 28th we could not find the smallest quantity of lactate of lime. If the of modern essays baudelaire, needs of industry should ever require the production of large quantities of butyric acid, there would, beyond doubt, be found in the preceding fact valuable information in devising an easy method of preparing that product in abundance. [Footnote: In what way are we to account for so great a difference between the two fermentations that we have just described? Probably it was owing to some modification effected in the medium by the previous life of the bacteria, or to the special character of the vibrios used in impregnation. Or, again, it might have been due to the action of the air, which, under the conditions of our second experiment, was not absolutely eliminated, since we took no precaution against its introduction at the moment of making family, filling our flask, and this would tend to facilitate the multiplication of anaerobian vibrios, just as, under similar conditions, would have been the case if we had been dealing with a fermentation by ordinary yeast.] Before we go any further, let us devote some attention to the vibrios of the of modern life, preceding fermentations. On May 27th, 1862, we completely filled a flask capable of chemosynthesis, holding 2.780 litres (about five pints) with the solution of lactate and phosphates. The Writer Essays On Charles Baudelaire. [Footnote: In this case the liquid was composed as follows: A saturated solution of lactate of lime, at a temperature of 25 degrees C. (77 degrees F.), was prepared, containing for every 1OO cc. (3 1/2 fl. oz.) 25.65 grammes (394 grains) of the lactate, C6 H5 O5 Ca O (NEW NOTATION, C6 H10 Ca O6) This solution was rendered very clear by the addition of 1 gramme of phosphate of ammonia and subsequent filtration. For a volume of 8 litres (14 pints) of this clear saturated solution we used (1 gramme = 15.43 grains): Phosphate of ammonia. Decision Making Family. . Life On Charles Baudelaire. . . . . . . 2 grammes. Phosphate of english the global, potassium. . . . Of Modern Life Essays Baudelaire. . . . . 1 gramme. Phosphate of magnesium. . . . . . . Family. . 1 gramme.
Sulphate of ammonia. . . . . . . . 0.5 gramme] We refrained from impregnating it with any germs. The liquid became turbid from the writer of modern essays on charles, a development of bacteria and then underwent butyric fermentation. By June 9th the fermentation had become sufficiently active to enable us to collect in the course of twenty-four hours, over mercury, as in all our experiments, about 100 cc. (about 6 cubic inches) of gas. By June 11th, judging from the volume of gas liberated in the course of twenty-four hours, the recovery business, activity of the fermentation had doubled. We examined a drop of the turbid liquid. Here are the notes accompanying the the writer life on charles baudelaire, sketch (Fig. 12) as they stand in our note-book: A swarm of lotf microcosm, vibrios, so active in their movements that the of modern life essays on charles baudelaire, eye has great difficulty in following them. They may be seen in pairs throughout the chemosynthesis, field, apparently making efforts to separate from essays on charles, each other. The connection would seem to be by some invisible, gelatinous thread, which yields so far to their efforts that they succeed in breaking away from actual contact, but yet are, for a while, so far restrained that the movements of one have a visible effect on those of the general, other. By and by, however, we see a complete separation effected, and essays each moves on an argumentative essay its separate way with an activity greater than it ever had before.
[Illustration with caption: Fig. 12] One of the best methods that can be employed for the. microscopical examination of these vibrios, quite out of contact with air, is the following. The Writer Of Modern Life Baudelaire. After butyric fermentation has been going on for several days in a flask, (Fig. 13), we connect this flask by microcosm an india-rubber tube with one of the flattened bulbs previously described, which we then place on the stage of the microscope (Fig.
13). When we wish to make an observation we close, under the mercury, at the point B, the the writer life essays on charles baudelaire, end of the drawn- out and bent delivery-tube. The continued evolution of different ways to write an argumentative essay, gas soon exerts such a pressure within the flask, that when we open the tap R, the liquid is the writer of modern essays baudelaire, driven into the bulb LL, until it becomes quite full and the liquid flows over into the glass V. In this manner we may bring the vibrios under observation without their coming into contact with the least trace of air, and with as much success as if the bulb, which takes the place of an object glass, had been plunged into data recovery business the very centre of the flask. The movements and fissiparous multiplication of the essays on charles baudelaire, vibrios may thus be seen in all their beauty, and papers english the global it is indeed a most interesting sight. The Writer Life Essays On Charles. The movements do not immediately cease when the temperature is suddenly lowered, even to decision making a considerable extent, 15 degrees C. (59 degrees F.) for example; they are only slackened. Nevertheless, it is better to observe them at the temperatures most favourable to fermentation, even in the oven where the vessels employed in the experiment are kept at a temperature between 25 degrees C. and 30 degrees C. Of Modern Life On Charles Baudelaire. (77 degrees F. and decision family 86 degrees F.). [Illustration: Fig. 13] We may now continue our account of the fermentation which we were studying when we made this last digression.
On June 17th that fermentation produced three times as much gas as it did on life essays June 11th, when the residue of hydrogen, after absorption by potash, was 72.6 per cent.; whilst on the 17th it was only 49.2 per cent. Let us again discuss the microscopic aspect of the turbid liquid at this stage. Appended is the sketch we made (Fig. 14) and our notes on it: A most beautiful object: vibrios all in motion, advancing or undulating. They have grown considerably in research papers english, bulk and length since the 11th; many of them are joined together in long sinuous chains, very mobile at the articulations, visibly less active and more wavering in of modern essays baudelaire, proportion to the number that go to form the chain, of the length of the individuals. This description is essay family, applicable to the majority of the vibrios which occur in cylindrical rods and are homogeneous in of modern essays baudelaire, aspect. There are others, of general formula, rare occurrence in chains, which have a clear corpuscle, that is to baudelaire say, a portion more refractive than other parts of the segments, at one of their extremities. Sometimes the microcosm, foremost segment has the life on charles baudelaire, corpuscle at one end, sometimes the other. The long segments of the different to write an argumentative essay, commoner kind attain a length of from the writer of modern essays baudelaire, 10 to 30 and even 45 thousandths of a millimetre. Solving Math Problems. Their diameter is from 1 1/2 to 2, very rarely 3, thousandths of a millimetre. [Footnote: 1 millimetre = 0.039 inch: hence the dimensions indicated will be--length, from the writer essays on charles baudelaire, 0.00039 to 0.00117, or even 0.00176 in.; diameter, from 0.000058 to 0.000078, rarely 0.000117 in.--D. C. R.] [Illustration: Figure 14.]
On June 28th, fermentation was quite finished; there was no longer any trace of gas, nor any lactate in solution. All the infusoria were lying motionless at the bottom of the flask. The liquid clarified by degrees, and in the course of a few days became quite bright. Here we may inquire, were these motionless infusoria, which from complete exhaustion of the lactate, the formula, source of the carbonaceous part of their food, were now lying inert at the bottom of the fermenting vessel--were they dead beyond the power of revival? [Footnote: The carbonaceous supply, as we remarked, had failed them, and to this failure the absence of vital action, nutrition, and multiplication was attributable. The liquid, however, contained butyrate of lime, a salt possessing properties similar to those of the lactate. Why could not this salt equally well support the life of the vibrios?
The explanation of the difficulty seems to us to lie simply in the fact that lactic acid produces heat by its decomposition, whilst butyric acid does not, and the vibrios seem to require heat during the the writer on charles, chemical process of their nutrition.] The following experiment leads us to solving math problems steps believe that they were not perfectly lifeless, and that they might behave in the same manner as the yeast of beer, which, after it has decomposed all the sugar in a fermentable liquid, is ready to revive and multiply in a fresh saccharine medium. Of Modern Life Essays. On April 22nd, 1875, we left in the oven at a temperature of 25 degrees C. (77 degrees F.) a fermentation of lactate of lime that had been completed. The delivery tube of the flask A, (Fig. 15), in which it had taken place, had never been withdrawn from under the mercury. We kept the liquid under observation daily, and saw it gradually become brighter; this went on for fifteen days. English The Global. We then filled a similar flask, B, with the solution of lactate, which we boiled, not only to kill the germs of vibrios which the liquid might contain, but also to expel the air that it held in solution. When the flask, B, had cooled, we connected the two flasks, avoiding the the writer essays baudelaire, introduction of air, [Footnote: To do this it is sufficient, first, to fill the curved ends of the stop-cocked tubes of the flasks, as well as the essay making family, india-rubber tube C C which connects them, with boiling water that contains no air.] after having slightly shaken the flask, A, to stir up the deposit at the bottom. There was then a pressure due to carbonic acid at the end of the delivery tube of this latter flask, at the point A, so that on the writer of modern life essays baudelaire opening the taps R and essay making S, the deposit at the writer life on charles the bottom of flask A was driven over into general formula chemosynthesis flask B, which in consequence was impregnated with the deposit of a fermentation that had been completed fifteen days before. Two days after impregnation the flask B began to show signs of the writer life on charles, fermentation.
It follows that the deposit of vibrios of a completed butyric fermentation may be kept, at least for a certain time, without losing the power of causing fementation. Math Problems. It furnishes a butyric ferment, capable of revival and action in a suitable fresh fermentable medium. [Illustration: Fig. 15] The reader who has attentively studied the facts which we have placed before him cannot, in our opinion, entertain the least doubt on the subject of the possible multiplication of the vibrios of a fermentation of lactate of lime out of contact with atmospheric oxygen. The Writer Of Modern Life. If fresh proofs of this important proposition were necessary, they might be found in the following observations, from which it may be inferred that atmospheric oxygen is ways to write, capable of suddenly checking a fermentation produced by butyric vibrios, and rendering them absolutely motionless, so that it cannot be necessary to enable them to live. Of Modern Life On Charles. On May 7th, 1862, we placed in essay decision, the oven a flask holding 2.580 litres (4 1/2 pints), and the writer of modern life filled with the solution of lactate of lime and phosphates, which we had impregnated on the 9th with two drops of a liquid in butyric fermentation. In the course of different ways to write an argumentative, a few days fermentation declared itself: on the writer life baudelaire the 18th it was active; on the 30th it was very active. On June 1st it yielded hourly 35 cc. (2.3 cubic inches) of gas, containing ten per cent, of hydrogen. On the 2nd we began the study of the action of air on the vibrios of this fermentation. To do this we cut off the delivery-tube on a level with its point of junction to the flask, then with a 50 cc. pipette we took out that quantity (1 3/4 fl. oz.) of liquid which was, of course, replaced at once by general air.
We then reversed the flask with the opening under the mercury, and shook it every ten minutes for more than an hour. Wishing to make sure, to begin with, that the oxygen had been absorbed we connected under the mercury the beak of the flask by means of a thin india-rubber tube filled with water, with a small flask, the neck of which had been drawn out and on charles baudelaire was filled with water; we then raised the large flask with the smaller kept above it. A Mohr's clip, which closed the india-rubber tube, and which we then opened, permitted the water contained in the small flask to pass into the large one, whilst the essay, gas, on the contrary, passed upwards from the large flask into the small one. We analyzed the gas immediately, and found that, allowing for the carbonic acid and hydrogen, it did not contain more than 14.2 per cent. of oxygen, which corresponds to an absorption of 6.6 cc., or of 3.3 cc. (0.2 cubic inch) of oxygen for essays on charles, the 50 cc. (3.05 cubic inches) of papers english language, air employed. Lastly, we again established connection by an india- rubber tube between the flasks, after having seen by microscopical examination that the movements of the vibrios were very languid. Fermentation had become less vigorous without having actually ceased, no doubt because some portions of the the writer of modern, liquid had not been brought into contact with the atmospheric oxygen, in spite of the prolonged shaking that the flask had undergone after the introduction of the air. Whatever the cause might have been, the significance of the phenomenon is not doubtful. To assure ourselves further of the effect of air on the vibrios, we half filled two test tubes with the fermenting liquid taken from another fermentation which had also attained its maximum of intensity, into one of which we passed a current of air, into the other carbonic acid gas. In the course of half an hour, all the vibrios in the aerated tube were dead, or at least motionless, and fermentation had ceased. In the other tube, after three hours' exposure to the effects of the carbonic acid gas, the vibrios were still very active, and fermentation was going on.
There is a most simple method of observing the general formula, deadly effect of the writer of modern essays on charles, atmospheric air upon vibrios. We have seen in the microscopical examination made by means of the apparatus represented in general chemosynthesis, FIG. 13, how remarkable were the movements of the vibrios when absolutely deprived of air, and how easy it was to discern them. We will repeat this observation, and at the same time make a comparative study of the same liquid under the microscope in the ordinary way, that is to say, by placing a drop of the liquid on an object-glass, and covering it with a thin glass slip, a method which must necessarily bring the of modern life on charles, drop into contact with air, if only for a moment. It is surprising what a remarkable difference is lotf, observed immediately between the movements of the vibrios in the bulb and those under the the writer, glass. In the case of the latter, we generally see all movement at once cease near the edges of the lotf microcosm essay, glass, where the drop of the writer of modern essays on charles, liquid is in direct contact with the air; the movements continue for a longer or shorter time about the centre, in proportion as the air is general formula chemosynthesis, more or less intercepted by the vibrios at the writer life essays the circumference of the liquid. It does not require much skill in experiments of this kind to formula chemosynthesis enable one to see plainly that immediately after the glass has been placed on the drop, which has been affected all over by atmospheric air, the whole of the vibrios seem to languish and to the writer of modern life on charles baudelaire manifest symptoms of illness--we can think of no better expression to explain what we see taking place--and that they gradually recover their activity about the centre, in proportion as they find themselves in a part of the medium that is recovery, less affected by the presence of oxygen. Some of the the writer life essays on charles, most curious facts are to be found in essay family, connection with an observation, the correlative and inverse of the foregoing, on the ordinary aerobian bacteria. If we examine below the microscope a drop of liquid full of these organisms under a coverslip, we very soon observe a cessation of motion in all the bacteria which lie in the writer of modern baudelaire, the central portion of the liquid, where the oxygen rapidly disappears to supply the necessities of the bacteria existing there; whilst, on the other hand, near the edges of the cover-glass the movements are very active, in consequence of the different an argumentative, constant supply of air.
In spite of the speedy death of the bacteria beneath the centre of the glass, we see life prolonged there if by chance a bubble of air has been enclosed. All round this bubble a vast number of bacteria collect in a thick, moving circle, but as soon as all the oxygen of the bubble has been absorbed they fall apparently lifeless, and are scattered by the writer of modern life on charles the movement of the liquid. Different To Write An Argumentative. [Footnote: We find this fact, which we published as long ago as 1863, confirmed in a work of H. Hoffman's, published in 1861 under the the writer life essays on charles, title of Memoire sur les bacteries, which has appeared in French (Annales des Sciences naturelles, 5th series, vol. ix.). On this subject we may cite an observation that has not yet been published. Aerobian bacteria lose all power of movement when suddenly plunged into carbonic acid gas; they recover it, however, as if they had only been suffering from anaesthesia, as soon as they are brought into the air again.] We may here be permitted to add, as a purely historical matter, that it was these two observations just described, made successively one day in 1861, on vibrios and bacteria, that first suggested to us the idea of the possibility of life without air, and caused us to think that the vibrios which we met so frequently in our lactic fermentations must be the true butyric ferment. We may pause to consider an interesting question in reference to the two characters under which vibrios appear in butyric fermentations. Different To Write An Argumentative. What is the reason that some vibrios exhibit refractive corpuscles, generally of a lenticular form, such as we see in FIG. 14. We are strongly inclined to believe that these corpuscles have to the writer of modern on charles do with a special mode of reproduction in the vibrios, common alike to the anaerobian forms which we are studying, and the ordinary aerobian forms in which also the corpuscles of which we are speaking may occur. The explanation of the phenomenon, from our point of essay, view, would be that, after a certain number of fisiparous generations, and under the influence of variations in the composition of the medium, which is constantly changing through fermentation as well as through the active life of the vibrios themselves, cysts, which are simply the refractive corpuscles, form along them at different points.
From these gemmules we have ultimately produced vibrios, ready to reproduce others by the process of transverse division for a certain time, to be themselves encysted, later on. Various observations incline us to believe that, in their ordinary form of the writer life essays on charles baudelaire, minute, soft, exuberant rods, the business, vibrios perish when submitted to desiccation, but when they occur in corpuscular or encysted form they possess unusual powers of the writer of modern life baudelaire, resistance and may be brought to the state of dry dust and be wafted about by winds. None of the matter which surrounds the corpuscle or cyst seems to take part in the preservation of the germ, when the cyst is solving math with, formed, for it is all re-absorbed, gradually leaving the cyst bare. The Writer On Charles. The cysts appear as masses of corpuscles, in which the most practiced eye cannot detect anything of an organic nature, or anything to remind one of the vibrios which produced them; nevertheless, these minute bodies are endowed with a latent vital action, and only await favourable conditions to develop long rods of vibrios. We are not, it is true, in a position to adduce any very forcible proofs in support of these opinions. Decision. They have been suggested to us by experiments, none of which, however, have been absolutely decisive in their favour. We may cite one of our observations on this subject. In a fermentation of glycerine in a mineral medium--the glycerine was fermenting under the influence of butyric vibrios--after we had determined the, we may say, exclusive presence of lenticular vibrios, with refractive corpuscles, we observed the fermentation, which for some unknown reason had been very languid, suddenly become extremely active, but now through the influence of the ordinary vibrios. The Writer Life On Charles. The gemmules with brilliant corpuscles had almost disappeared; we could see but very few, and those now consisted of the refractive bodies alone, the bulk of the solving problems with, vibrios accompanying them having undergone some process of re-absorption. Another observation which still more closely accords with this hypothesis is given in our work on silk-worm disease (vol.
1, p. Life. 256). Essay Family. We there demonstrated that, when we place in water some of the dust formed of desiccated vibrios, containing a host of these refractive corpuscles, in the course of a very few hours large vibrios appear, well-developed rods fully grown, in which the brilliant points are absent; whilst in the water no process of development from smaller vibrios is to be discerned, a fact which seems to show that the former had issued fully grown from the refractive corpuscles, just as we see colpoda issue with their adult aspect from the dust of their cysts. This observation, we may remark, furnishes one of the best proofs that can be adduced against the spontaneous generation of vibrios or bacteria, since it is probable that the same observation applies to bacteria. It is the writer life on charles, true that we cannot say of mere points of research the global language, dust examined under the microscope, that one particular germ belongs to vibrio, another to bacterium; but how is it possible to doubt that the vibrios issue, as we see them, from an ovum of the writer of modern life on charles, some kind, a cyst, or germ, of determinate character, when, after having placed some of those indeterminate motes of dust into essay decision family clean water, we suddenly see, after an interval of not more than one or two hours, an adult vibrio crossing the field of the microscope, without our having been able to detect any intermediate state between its birth and adolescence? [Illustration: Fig. 16]
It is a question whether differences in the aspect and nature of vibrios, which depend upon their more or less advanced age, or are occasioned by the influence of the writer of modern life on charles baudelaire, certain conditions on the medium in which they propagate, do not bring about corresponding changes in the course of the fermentation and the nature of its products. Judging at least from the variations in the proportions of hydrogen, and carbonic acid gas produced in ways essay, butyric fermentations, we are inclined to think that this must be the case; nay, more, we find that hydrogen is not even a constant product in these fermentations. We have met with butyric fermentations of the writer of modern life essays, lactate of lime which did not yield the minutest trace of hydrogen, or anything besides carbonic acid. Fig. Data Recovery. 16 represents the of modern life baudelaire, vibrios which we observed in a fermentation of this kind. They present no special features.
Butyl alcohol is, according to our observations, an ordinary product, although it varies and is by no means a necessary concomitant of these fermentations. It might be supposed, since butylic alcohol may be produced and business plan hydrogen be in deficit, that the proportion of the former of these products would attain its maximum when the latter assumed a minimum. This, however, is by no means the case; even in those few fermentations that we have met with in which hydrogen was absent, there was no formation of butylic alcohol. From a consideration of all the facts detailed in this section we can have no hesitation in of modern life essays on charles baudelaire, concluding that, on the one hand, in cases of butyric fermentation, the vibrios which abound in lotf essay, them and constitute their ferment, live without air or free oxygen; and that, on the other hand, the presence of on charles baudelaire, gaseous oxygen operates prejudicially against the movements and activity of those vibrios. But how does it follow that the presence of minute quantities of air brought into math with steps contact with a liquid undergoing butyric fermention would prevent the continuance of that fermentation or even exercise any check upon it? We have not made any direct experiments upon this subject; but we should not be surprised to find that, so far from hindering, air may, under such circumstances, facilitate the propagation of the vibrios and accelerate fermentation. This is exactly what happens in the case of yeast. But how could we reconcile this, supposing it were proved to be the case, with the fact just insisted on as to the writer of modern life the danger of bringing the butyric vibrios into contact with air? It may be possible that LIFE WITHOUT AIR results from habit, whilst DEATH THROUGH AIR may be brought about by a sudden change in the conditions of the existence of the vibrios. The following remarkable experiment is general, well-known: A bird is placed in the writer of modern life essays on charles, a glass jar of general chemosynthesis, one or two litres (60 to 120 cubic inches) in capacity which is baudelaire, then closed. Solving With Steps. After a time the creature shows every sign of intense uneasiness and asphyxia long before it dies; a similar bird of the same size is introduced into the jar; the death of the latter takes place instanteously, whilst the life of the the writer essays on charles baudelaire, former may still be prolonged under these conditions for a considerable time, and there is no, difficulty even in restoring the bird to perfect health by taking it out of the jar.
It seems impossible to deny that we have here a case of the adaptation of an organism to general formula chemosynthesis the gradual contamination of the medium; and so it may likewise happen that the anaerobian vibrios of a butyric fermentation, which develop and the writer of modern multiply absolutely without free oxygen, perish immediately when suddenly taken out of their airless medium, and that the result might be different if they had been gradually brought under the action of air in small quantities at a time. We are compelled here to admit that vibrios frequently abound in liquids exposed to the air, and that they appropriate the atmospheric oxygen, and could not withstand a sudden removal from its influence. Must we, then, believe that such vibrios are absolutely different from those of butyric fermentations? It would, perhaps, be more natural to admit that in the one case there is an adaptation to lotf microcosm essay life with air, and in the other case an adaptation to life without air; each of the varieties perishing when suddenly transferred from its habitual condition to that of the other, whilst by a series of progressive changes one might be modified into the other. [Footnote: These doubts might be easily removed by putting the matter to the test of direct experiment.] We know that in the case of alcoholic ferments, although these can actually live without air, propagation is life essays, wonderfully assisted by decision the presence of on charles baudelaire, minute quantities of air; and certain experiments which we have not yet published lead us to believe that, after having lived without air, they cannot be suddenly exposed with impunity to the influence of large quantities of oxygen. We must not forget, however, that aerobian torulae and anaerobian ferments present an example of organisms apparently identical, in which, however, we have not yet been able to discover any ties of a common origin. Hence we are forced to regard them as a distinct species; and so it is possible that there may likewise be aerobian and anaerobian vibrios without any transformation of the one into the other. The question has been raised whether vibrios, especially those which we have shown to be the ferment of butyric and many other fermentations, are in general formula, their nature, animal or vegetable. M. Ch. Robin attaches great importance to the solution of baudelaire, this question, of which he speaks as follows: [Footnote: ROBIN, Sur la nature des fermentations, c. (Journal de l'Academie et de la Physiologie, July and August, 1875, P. 386).] The determination of the nature, whether animal or vegetable, of organisms, either as a whole or in respect to their anatomical parts, assimilative or reproductive, is a problem which has been capable of decision family, solution for a quarter of a century. The Writer Of Modern Life Essays Baudelaire. The method has been brought to a state of remarkable precision, experimentally, as well as in its theoretical aspects, since those who devote their attention to the organic sciences consider it indispensable in every observation and data business experiment to the writer on charles baudelaire determine accurately, before anything else, whether the object of their study is animal or vegetable in its nature, whether adult or otherwise.
To neglect this is as serious an different ways to write an argumentative essay, omission for such students as for the writer essays baudelaire, chemists would be the neglecting to business plan determine whether it is nitrogen or hydrogen, urea or stearine, that has been extracted from a tissue, or which it is whose combinations they are studying in this or that chemical operation. The Writer On Charles Baudelaire. Now, scarcely any one of those who study fermentations, properly so-called, and putrefactions, ever pay any attention to the preceding data. . Among the observers to whom I allude, even M. Pasteur is to be found, who, even in his most recent communications, omits to state definitely what is the nature of many of the essay decision family, ferments which he has studied, with the exception, however, of those which belong to the cryptogamic group called torulaceae. Various passages in his work seem to the writer essays show that he considers the cryptogamic organisms called bacteria, as well as those known as vibrios, as belonging to the animal kingdom (see Bulletin de l'Academie de Medecine, Paris, 1875, pp. 249, 251, especially 256, 266, 267, 289, and 290). These would be very different, at least physiologically, the former being anaerobian, that is to say, requiring no air to enable them to live, and being killed by oxygen, should it be dissolved in the liquid to any considerable extent. We are unable to see the matter in the same light as our learned colleague does; to our thinking, we should be labouring under a great delusion were we to decision making family suppose that it is quite as serious an omission not to determine the animal or vegetable nature of a ferment as it would be to confound nitrogen with hydrogen or urea with stearine. The importance of the solutions of disputed questions often depends on the writer life on charles the point of ways an argumentative, view from which these are regarded. The Writer Life On Charles. As far as the result of our labours is concerned, we devoted our attention to these two questions exclusively: 1. Ways An Argumentative Essay. Is the ferment, in every fermentation properly so called, an the writer of modern baudelaire, organized being? 2. Decision Making Family. Can this organized being live without air?
Now, what bearing can the of modern life essays on charles, question of the animal or vegetable nature of the ferment, of the organized being, have upon the investigation of these two problems? In studying butyric fermentation, for example, we endeavoured to establish these two fundamental points; 1. The BUTYRIC FERMENT IS A VIBRIO. 2. THIS VIBRIO MAY DISPENSE WITH AIR IN ITS LIFE, AND, AS A MATTER OF FACT, DOES DISPENSE WITH IT IN THE ACT OF PRODUCING BUTYRIC FERMENTATION. We did not consider it at different to write essay all necessary to pronounce any opinion as to the animal or vegetable nature of this organism, and, even up to the present moment, the idea that vibrio is an animal and not a plant is in our minds, a matter of sentiment rather than of conviction. M. Of Modern Life Essays On Charles. Robin, however, would have no difficulty in determining the limits of the two kingdoms. According to him, every variety of cellulose is, we may say, insoluble in ammonia, as also are the reproductive elements of plants, whether male or female. Whatever phase of evolution the elements which reproduce a new individual may have reached, treatment with this reagent, either cold or raised to research papers boiling, leaves them absolutely intact under the eyes of the observer, except that their contents, from being partially dissolved, become more transparent. Every vegetable whether microscopic or not, every mycelium and every spore, thus preserves in its entirety its special characteristics of form, volume and the writer of modern essays structural arrangements; whilst in decision making, the case of microscopic animals, or the ova and microscopic embryos of different members of the animal kingdom, the very opposite is the case.
We should be glad to learn that the employment of a drop of the writer essays on charles baudelaire, ammonia would enable us to pronounce an opinion with this degree of confidence on the nature of the lowest microscopic beings; but is M. Robin absolutely correct in his assumptions? That gentleman himself remarks that spermatozoa, which belong to animal organisms, are insoluble in ammonia, the effect of which is merely to make them paler. If a difference of action in certain reagents, in ammonia, for example, were sufficient to determine the limits of the animal and vegetable kingdoms, might we not argue that there must be a very great and natural difference between moulds and bacteria, inasmuch as the presence of a small quantity of acid in the nutritive medium facilitates the growth and propagation of the former, whilst it is able to prevent the life of bacteria and vibrios? Although as is the global, well known, movement is not an exclusive characteristic of of modern life essays on charles baudelaire, animals, yet we have always been inclined to regard vibrios as animals, on account of the peculiar character of their movements. How greatly they differ in this respect from the diatomacae, for example! When the vibrio encounters an obstacle it turns, or after assuring itself by some visual effort or other that it cannot overcome it, it retraces its steps. The colpoda--undoubted infusoria--behave in an exactly similar manner. It is true one may argue that the zoospores of certain cryptogamia exhibit similar movements; but do not these zoospores possess as much of an animal nature as do the spermatozoa? As far as bacteria are concerned, when, as already remarked, we see them crowd round a bubble of air in a liquid to prolong their life, oxygen having failed them everywhere else, how can we avoid believing that they are animated by an instinct for life, of the same kind that we find in animals? M. Robin seems to us to be wrong in supposing that it is possible to draw any absolute line of separation between the animal and vegetable kingdoms.
The settlement of this line however, we repeat again, no matter what it may be, has no serious bearing upon the questions that have been the subject of our researches. In like manner the difficulty which M. Robin has raised in objecting to the employment of the word GERM, when we cannot specify whether the nature of that germ is animal or vegetable, is in essay decision making, many respects an unnecessary one. In all the questions which we have discussed, whether we were speaking of fermentation or spontaneous generation, the word GERM has been used in the sense of ORIGIN OF LIVING ORGANISM. If Liebig, for example, said of an albuminous substance that it gave birth to ferment, could we contradict him more plainly than by replying No; ferment is an organized being, the germ of essays baudelaire, which is always present, and the albuminous substance merely serves by its occurrence to nourish the germ and its successive generations? In our Memoir of 1862, on so-called SPONTANEOUS generations, would it not have been an entire mistake to have attempted to assign specific names to the microscopic organisms which we met with in different an argumentative essay, the course of our observations? Not only would we have met with extreme difficulty in the attempt, arising from the state of extreme confusion which even in life essays on charles baudelaire, the present day exists in the classification and nomenclature of these microscopic organisms, but we should have been forced to sacrifice clearness in our work besides; at all events, we should have wandered from our principal object, which was the determination of the presence or absence of life in formula chemosynthesis, general, and had nothing to do with the manifestation of a particular kind of life in this or that species, animal or vegetable. Thus we have systematically employed the vaguest nomenclature, such as mucors, torulae, bacteria, and vibrios. There was nothing arbitrary in our doing this, whereas there is the writer of modern, much that is recovery business, arbitrary in adopting a definite system of nomenclature, and applying it to organisms but imperfectly known, the differences or resemblances between which are only recognizable through certain characteristics, the true signification of which is obscure. Take, for example, the extensive array of widely different systems which have been invented during the last few years for the species of the of modern life essays baudelaire, genera bacterium and vibrio in the works of Cohn, H. Hoffmann, Hallier, and Billroth.
The confusion which prevails here is ways to write an argumentative essay, very great, although we do not of course by any means place these different works on the same footing as regards their respective merits. M. Robin is, however, right in recognizing the impossibility of maintaining in the present day, as he formerly did, That fermentation is an exterior phenomenon, going on outside cryptogamic cells, a phenomenon of contact. It is probably, he adds, an interior and the writer life on charles molecular action at work in the innermost recesses of the general, substance of each cell. From the of modern life essays on charles, day when we first proved that it is possible for all organized ferments, properly so called, to spring up and multiply from solving steps for free, their respective germs, sown, whether consciously or by accident, in a mineral medium free from organic and the writer of modern on charles baudelaire nitrogenous matters other than ammonia, in which medium the fermentable matter alone is adapted to microcosm provide the ferment with whatever carbon enters into its composition, from that time forward the theories of of modern life essays on charles baudelaire, Liebig, as well of Berzelius, which M. Robin formerly defended, have had to give place to others more in harmony with facts. We trust that the research english the global language, day will come when M. Robin will likewise acknowledge that he has been in error on the subject of the doctrine of spontaneous generation, which he continues to the writer essays baudelaire affirm, without adducing any direct proofs in support of it, at the end of the article to which we have been here replying. We have devoted the greater part of this chapter to the establishing with all possible exactness the extremely important physiological fact of life without air, and its correlation to the phenomena of fermentations properly so called--that is to say, of those which are due to essay decision making the presence of the writer of modern on charles, microscopic cellular organisms. This is the chief basis of the new theory that we propose for the explanation of these phenomena. The details into data recovery which we have entered were indispensable on account of the novelty of the subject no less than on account of the necessity we were under of combating the the writer on charles baudelaire, criticisms of the two German naturalists, Drs. Oscar Brefeld and Traube, whose works had cast some doubts on the correctness of the facts upon research papers english, which we had based the preceding propositions. We have much pleasure in adding that at the very moment we were revising the proofs of this chapter, we received from M. Brefeld an life essays baudelaire, essay, dated Berlin, January, 1876, in which, after describing his later experimental researches, he owns with praiseworthy frankness that Dr.
Traube and he were both of them mistaken. Life without air is now a proposition which he accepts as perfectly demonstrated. He has witnessed it in the case of Mucor racemosus and has also verified it in the case of yeast. If, he says, after the results of my previous researches, which I conducted with all possible exactness, I was inclined to consider Pasteur's assertion as inaccurate and to attack them, I have no hesitation now in recognizing them as true, and in proclaiming the service which Pasteur has rendered to science in being the first to indicate the exact relation of things in the phenomenon of fermentation. In his later researches, Dr.
Brefeld has adopted the method which we have long employed for demonstrating the life and multiplication of butyric vibrios in the entire absence of air, as well as the method of conducting growths in mineral media associated with fermentable substance. We need not pause to consider certain other secondary criticisms of Dr. Brefeld. A perusal of the present work will, we trust, convince him that they are based on no surer foundation than were his former criticisms. To bring one's self to believe in a truth that has just dawned upon one is the first step towards progress; to persuade others is the second. Recovery Business Plan. There is a third step, less useful perhaps, but highly gratifying nevertheless, which is, to convince one's opponents. We therefore, have experienced great satisfaction in learning that we have won over to our ideas an observer of singular ability, on a subject which is of the utmost importance to the physiology of cells. VI. REPLY TO THE CRITICAL OBSERVATIONS OF LIEBIG, PUBLISHED IN 1870. [Footnote: LIEBIG, Sur la fermentation et la source de la force musculaire (Annales de Chimie et de Physique, 4th series, t. xxiii., p. 5, 1870).]
In the of modern essays on charles baudelaire, Memoir which we published, in 1860, on alcoholic fermentation, and in several subsequent works, we were led to a different conclusion on the causes of this very remarkable phenomenon from that which Liebig had adopted. The opinions of Mitscherlich and decision making Berzelius had ceased to be tenable in the presence of the new facts which we had brought to light. From that time we felt sure that the the writer life essays baudelaire, celebrated chemist of Munich had adopted our conclusions, from the fact that he remained silent on this question for a long time, although it had been until then the constant subject of his study, as is shown by all his works. Suddenly there appeared in the Annales de Chimie et de Physique a long essay, reproduced from a lecture delivered by him before the ways an argumentative, Academy of the writer of modern on charles baudelaire, Bavaria in 1868 and papers english 1869. In this Liebig again maintained, not, however, without certain modifications, the views which he had expressed in his former publications, and of modern essays on charles disputed the correctness of the principal facts enunciated in research, our Memoir of 1860, on which were based the arguments against his theory. I had admitted, he says, that the resolution of fermentable matter into compounds of the writer life essays on charles, a simpler kind must be traced to papers the global language some process of decomposition taking place in the ferment, and that the action of of modern, this same ferment on the fermentable matter must continue or cease according to the prolongation or cessation of the alteration produced in the ferment. Solving Problems. The molecular change in the sugar, would, consequently, be brought about by the destruction or modification of one or more of the component parts of the life, ferment, and could only ways, take place through the contact of the two substances.
M. Of Modern Life On Charles. Pasteur regards fermentation in the following light: The chemical action of fermentation is essentially a phenomenon correlative with a vital action, beginning and to write an argumentative essay ending with it. He believes that alcoholic fermentation can never occur without the simultaneous occurrence of organization, development, and multiplication of globules, or continuous life, carried on from globules already formed. But the the writer life on charles baudelaire, idea that the decomposition of sugar during fermentation is due to the development of the cellules of the ferment, is in contradiction with the fact that the ferment is able to bring about the fermentation of a pure solution of sugar. The greater part of the ferment is composed of a substance that is rich in english the global language, nitrogen and contains sulphur. It contains, moreover, an appreciable quantity of phosphates, hence it is difficult to the writer of modern life on charles baudelaire conceive how, in the absence of these elements in a pure solution of sugar undergoing fermentation, the number of cells is capable of any increase. Notwithstanding Liebig's belief to the contrary, the idea that the decomposition of sugar during fermentation is math problems steps, intimately connected with a development of the cellules of the essays on charles baudelaire, ferment, or a prolongation of the life of cellules already formed, is in no way opposed to the fact that the ferment is capable of bringing about the different to write an argumentative essay, fermentation of a pure solution of sugar. It is manifest to any one who has studied such fermentation with the microscope, even in those cases where the sweetened water has been absolutely pure, that ferment-cells do multiply, the reason being that the cells carry with them all the food-supplies necessary for the life of the ferment. They may be observed budding, at least many of them, and there can be no doubt that those which do not bud still continue to life baudelaire live; life has other ways of manifesting itself besides development and cell-proliferation. If we refer to the figures on page 81 of our Memoir of 1860, Experiments D, E, F, H, I, we shall see that the general, weight of of modern life on charles baudelaire, yeast, in the case of the fermentation of a pure solution of sugar, undergoes a considerable increase, even without taking into account the decision, fact that the the writer life, sugared water gains from the yeast certain soluble parts, since in the experiments just mentioned, the weights of solid yeast, washed and different dried at 100 degrees C. (212 degrees F.), are much greater than those of the raw yeast employed, dried at the same temperature.
In these experiments we employed the of modern life essays on charles baudelaire, following weights of yeast, expressed in to write essay, grammes (1 gramme=15.43 grains): which became, after fermentation, we repeat, without taking into. account the matters which the sugared water gained from the. (1) 2.486 Increase 0.173 = 2.65. (2) 2.963 Increase 0.337 = 5.16. (3) 1.700 Increase 0.502 = 7.7. (4) 0.712 Increase 0.013 = 0.2. (5) 0.325 Increase 0.009 = 0.14. (6) 0.590 Increase 0.114 = 1.75. Have we not in this marked increase in weight a proof of life, or, to adopt an expression which may be preferred, a proof of a profound chemical work of the writer on charles baudelaire, nutrition and assimilation?
We may cite on this subject one of our earlier experiments, which is to be found in the Comptes rendus de l'Academie for the year 1857, and which clearly shows the great influence exerted on fermentation by the soluble portion that the different ways an argumentative, sugared water takes up from the the writer essays on charles, globules of ferment: We take two equal quantities of fresh yeast that have been washed very freely. Essay Decision Making Family. One of these we cause to ferment in of modern baudelaire, water containing nothing but sugar, and, after removing from the general formula chemosynthesis, other all its soluble particles--by boiling it in an excess of water and then filtering it to separate the globules--we add to the filtered liquid as much sugar as was used in the first case along with a mere trace of fresh yeast insufficient, as far as its weight is of modern life essays, concerned, to general affect the of modern life essays on charles baudelaire, results of our experiment. Essay. The globules which we have sown bud, the liquid becomes turbid, a deposit of yeast gradually forms, and, side by side with these appearances, the decomposition of the sugar is effected, and in the course of life essays, a few hours manifests itself clearly. These results are such as we might have anticipated. The following fact, however, is of importance. In effecting by these means the general formula chemosynthesis, organization into globules of the soluble part of the yeast that we used in the second case, we find that a considerable quantity of sugar is essays, decomposed.
The following are the results of our experiment; 5 grammes of yeast caused the fermentation of 12.9 grammes of sugar in six days, at the end of which time it was exhausted. The soluble portion of a like quantity of 5 grammes of the microcosm essay, same yeast caused the of modern essays on charles baudelaire, fermentation of 10 grammes of sugar in nine days, after which the yeast developed by microcosm the sowing was likewise exhausted. How is it possible to maintain that, in the fermentation of water containing nothing but sugar, the soluble portion of the yeast does not act, either in the production of life essays, new globules or the research papers the global, perfection of old ones, when we see, in the preceding experiment, that after this nitrogenous and mineral portion has been removed by boiling, it immediately serves for the production of new globules, which, under the influence of the sowing of a mere trace of of modern on charles baudelaire, globules, causes the fermentation of so much sugar? [Footnote: It is important that we should here remark that, in general formula chemosynthesis, the fermentation of pure solution of sugar by means of yeast, the of modern life essays baudelaire, oxygen originally dissolved in the water, as well as that appropriated by the globules of yeast in their contact with air, has a considerable effect on the activity of the fermentation. As a matter of fact, if we pass a strong current of carbonic acid through the sugared water and the water in general formula chemosynthesis, which the yeast has been treated, the fermentation will be rendered extremely sluggish, and the few new cells of yeast which form will assume strange and abnormal aspects. Indeed this might have been expected, for of modern essays baudelaire, we have seen that yeast, when somewhat old, is incapable of development or of causing fermentation even in a fermentable medium containing all the nutritive principles of yeast if the liquid has been deprived of air; much more should we expect this to essay decision making be the case in pure sugared water, likewise deprived of air.] In short, Liebig is not justified in saying that the solution of pure sugar, caused to ferment by means of yeast, contains none of the elements needed for the growth of of modern essays baudelaire, yeast, neither nitrogen, sulphur nor phosphorus, and that, consequently, it should not be possible, by our theory, for the sugar to ferment. On the contrary, the solution does contain all these elements, as a consequence of the introduction and presence of the yeast.
Let us proceed without examination of Liebig's criticisms: To this, he goes on to say, must be added the microcosm essay, decomposing action which yeast exercises on the writer essays a great number of family, substances, and which resembles that which sugar undergoes. I have shown that malate of lime ferments readily enough through the action of yeast, and that it splits up into three other calcareous salts, namely, the acetate, the carbonate and the writer life baudelaire the succinate. If the data recovery plan, action of the writer of modern life essays, yeast consists in lotf essay, its increase and of modern essays baudelaire multiplication, it is difficult to lotf essay conceive this action in the case of malate of lime and other calcareous salts of the writer of modern life, vegetable acids. This statement, with all due deference to the opinion of our illustrious critic, is by no means correct. Yeast has no action on malate of lime, or on other calcareous salts formed by vegetable acids.
Liebig had previously, much to his own satisfaction, brought forward urea as being capable of transformation into carbonate of ammonia during alcoholic fermentation in contact with yeast. This has been proved to be erroneous. Research Papers Language. It is an error of the same kind that Liebig again brings forward here. Of Modern Life. In the fermentation of which he speaks (that of malate of lime), certain spontaneous ferments are produced, the germs of which are associated with the yeast, and develop in the mixture of yeast and malate. The yeast merely serves as a source of data recovery business, food for these new ferments without taking any direct part in the writer of modern life essays on charles, the fermentations of which we are speaking. Our researches leave no doubt on this point, as is evident from the different ways, observations on the fermentation of tartrate of lime previously given. It is true that there are circumstances under which yeast brings about modifications in different substances. Life Essays On Charles. Doebereiner and, Mitscherlich, more especially, have shown that yeast imparts to water a soluble material, which liquefies cane-sugar and produces inversion in it by different to write essay causing it to take up the the writer on charles baudelaire, elements of water, just as diastase behaves to starch or emulsin to amygdalin. M. Berthelot also has shown that this substance may be isolated by precipitating it with alcohol, in the same way as diastase is precipitated from its solutions. [Footnote: DOEBEREINER, Journal de Chimie de Schweigger, vol. Different To Write An Argumentative. xii., p. 129, and Journal de Pharmacie, vol. i., p. 342. MITSCHERLICH, Monatsberichte d. Kon. Preuss.
Akad. d. Wissen, eu Berlin, and Rapports annuels da Berzelius, Paris, 1843, 3rd year. The Writer Life On Charles. On the occasion of a communication on the inversion of cane-sugar by H. Rose, published in 1840, M. Mitscherlich observed: The inversion of cane-sugar in alcoholic fermentation is not due to the globules of yeast, but to a soluble matter in the water with which they mix. Lotf Essay. The liquid obtained by of modern essays baudelaire straining off the ferment on a filter paper possesses the property of converting cane-sugar into uncrystallizable sugar. BERTHELOT, Comptes rendus de l'Academie. Meeting of papers english the global language, May 28th, 1860, M. Berthelot confirms the preceding experiment of Mitscherlich, and proves, moreover, that the soluble matter of which the author speaks may be precipitated with alcohol without losing its invertive power.
M. Of Modern Essays On Charles. Bechamp has applied Mitscherlich's observation, concerning the soluble fermentative part of yeast, to fungoid growths, and has made the interesting discovery that fungoid growths, like yeast, yield to water a substance that inverts sugar. When the production of data recovery, fungoid growths is prevented by means of an antiseptic, the inversion of sugar does not take place. We may here say a few words respecting M. Bechamp's claim to priority of discovery. It is a well-known fact that we were the first to demonstrate that living ferments might be completely developed if their germs were placed in pure water together with sugar, ammonia, and phosphates. Relying on this established fact, that moulds are capable of development in sweetened water in the writer of modern life on charles baudelaire, which, according to M. Bechamp, they invert the sugar, our author asserts that he has proved that living organized ferments may originate in media which contain no albuminous substances. (See Comptes rendus, vol. Family. ixxv., p. 1519.) To be logical, M. Bechamp might say that he has proved that certain moulds originate in pure sweetened water without nitrogen or phosphates or other mineral elements, for such a deduction might very well be drawn from his work, in the writer essays baudelaire, which we do not find the least expression of astonishment at the possibility of essay making, moulds developing in pure water containing nothing but sugar without other mineral or organic principles. M. Bechamp's first note on the inversion of sugar was published in 1855. In it we find nothing relating to the influence of moulds. His second, in the writer baudelaire, which that influence is ways to write essay, noticed, was published in January, 1858, that is, subsequently to our work on lactic fermentation, which appeared in November, 1857. In that work we established for the first time that the lactic ferment is a living, organized being, that albuminous substances have no share in the production of essays on charles baudelaire, fermentation, and that they only serve as the food of the ferment. M. Bechamp's note was even subsequent to our first work on microcosm alcoholic fermentation, which appeared on the writer of modern life December 21st, 1857. It is since the math problems steps for free, appearance of these two works of ours that the preponderating influence of the life of microscopic organism in the phenomena of fermentation has been better understood.
Immediately after their appearance M. Bechamp, who from the writer baudelaire, 1855 had made no observation on the action of fungoid growths on chemosynthesis sugar, although he had remarked their presence, modified his former conclusions. (Comptes rendus, January 4th, 1858.)] These are remarkable facts, which are, however, at present but vaguely connected with the alcoholic fermentation of of modern life on charles baudelaire, sugar by means of yeast. The researches in which we have proved the existence of special forms of living ferments in solving problems, many fermentations, which one might have supposed to have been produced by simple contact action, had established beyond doubt the existence of profound differences between those fermentations, which we have distinguished as fermentations proper, and the phenomena connected with soluble substances. The more we advance, the more clearly we are able to detect these differences. M. Dumas has insisted on the fact that the ferments of fermentation proper multiply and reproduce themselves in the process whilst the others are destroyed. [Footnote: There are two classes of life, ferments; the first, of which the yeast of beer may be taken as the type, perpetuate and renew themselves if they can find in the liquid in which they produce fermentation food enough for their wants; the math with for free, second, of which diastase is the type, always sacrifice themselves in the exercise of their activity. (DUMAS, Comptes rendus de l'Academie, t. lxxv., p. 277, 1872.)] Still more recently M. Muntz has shown that chloroform prevents fermentations proper, but does not interfere with the action of diastase (Comptes rendus, 1875). M. Essays. Bouchardat had already established the fact that hydrocyanic acid, salts of mercury, ether, alcohol, creosote, and the oils of turpentine, lemon, cloves, and mustard destroy or check alcoholic fermentations, whilst in no way interfering with the glucoside fermentations (Annales de Chimie et de Physique. 3rd series, t. xiv., 1845). We may add in praise of M. Bouchardat's sagacity, that that skilful observer has always considered these results as a proof that alcoholic fermentation is dependent on the life of the yeast-cell, and that a distinction should be made between the two orders of fermentation.
M. Paul Bert, in general formula, his remarkable studies on the influence of barometric pressure on the phenomena of life, has recognized the fact that compressed oxygen is fatal to certain ferments, whilst under similar conditions it does not interfere with the action of those substances classed under the name of SOLUBLE FERMENTS, such as diastase (the ferment which inverts cane sugar) emulsin and others. The Writer Of Modern Life On Charles Baudelaire. During their stay in compressed air, ferments proper ceased their activity, nor did they resume it, even after exposure to air at ordinary pressures, provided the different ways to write, access of germs was prevented. We now come to Liebig's principal objection, with which he concludes his ingenious argument, and to which no less than eight or nine pages of the Annales are devoted. Our author takes up the question of the possibility of causing yeast to the writer of modern life on charles baudelaire grow in general formula chemosynthesis, sweetened water, to which a salt of ammonia and some yeast-ash have been added--a fact which is evidently incompatible with his theory that a ferment is always an albuminous substance on the writer of modern baudelaire its way to decomposition. In this case the albuminous substance does not exist; we have only the recovery business, mineral substances which will serve to produce it. We know that Liebig regarded yeast, and, generally speaking, any ferment whatever, as being a nitrogenous, albuminous substance which, in the same way as emulsin, for example, possesses the power of bringing about of modern life essays on charles, certain chemical decompositions. He connected fermentation with the easy decomposition of that albuminous substance, and imagined that the phenomenon occurred in the following manner: The albuminous substance on its way to decomposition possesses the power of communicating to certain other bodies that same state of mobility by which its own atoms are already affected; and through its contact with other bodies it imparts to different ways an argumentative essay them the of modern essays baudelaire, power of decomposing or of entering into other combinations. Here Liebig failed to perceive that the data, ferment, in its capacity of a living organism, had anything to of modern on charles baudelaire do with the fermentation. This theory dates back as far as 1843. Essay Decision Making. In 1846 Messrs.
Boutron and the writer life on charles Fremy, in a Memoir on formula chemosynthesis lactic fermentation, published in the Annales de Chimie et de Physique, strained the conclusions deducible from of modern essays on charles baudelaire, it to a most unjustifiable extent. They asserted that one and the same nitrogenous substance might undergo various modifications in contact with air, so as to lotf essay become successively alcoholic, lactic, butyric, and other ferments. Of Modern Life Essays Baudelaire. There is nothing more convenient than purely hypothetical theories, theories which are not the necessary consequences of facts; when fresh facts which cannot be reconciled with the original hypothesis are discovered, new hypotheses can be tacked on to the old ones. This is exactly what Liebig and Fremy have done, each in his turn, under the pressure of our studies, commenced in 1857. In 1864 Fremy devised the theory of hemi-organism, which meant nothing more than that he gave up Liebig's theory of 1843, together with the additions which Boutron and he had made to it in 1846; in data business, other words, he abandoned the idea of the writer life essays on charles, albuminous substances being ferments, to take up another idea, that albuminous substances in contact with air are peculiarly adapted to undergo organization into new beings--that is, the living ferments which we had discovered--and that the ferments of beer and of the grape have a common origin. This theory of hemi-organism was word for word the antiquated opinion of Turpin. Different Ways Essay. * * * The public, especially a certain section of the public did not go very deeply into an examination of the subject. It was the period when the doctrine of spontaneous generation was being discussed with much warmth. The new word hemi-organism, which was the life essays, only novelty in M. Fremy's theory, deceived people. Solving Math Problems With For Free. It was thought that M. Fremy had really discovered the solution of the question of the day. Essays. It is true that it was rather difficult to understand the process by which an general chemosynthesis, albuminous substance could become all at once a living and the writer essays budding cell. This difficulty was solved by lotf essay M. The Writer Baudelaire. Fremy, who declared that it was the result of some power that was not yet understood, the power of data recovery business, organic impulse. The Writer Of Modern Life On Charles Baudelaire. [Footnote: FREMY, Comptes rendus de l'Academie, vol. lviii., p. 1065, 1864.]
Liebig, who, as well as M. Fremy, was compelled to renounce his original opinions concerning the nature of ferments, devised the following obscure theory (Memoir by Liebig, 1870, already cited): There seems to be no doubt as to the part which the vegetable organism plays in the phenomenon of fermentation. It is through it alone that an albuminous substance and sugar are enabled to unite and form this particular combination, this unstable form under which alone, as a component part of the mycoderm, they manifest an steps for free, action on sugar. Should the mycoderm cease to grow, the the writer life essays on charles, bond which unites the constituent parts of the microcosm essay, cellular contents is loosened, and it is through the motion produced therein that the the writer essays on charles, cells of yeast bring about a disarrangement or separation of the elements of the sugar into molecules. One might easily believe that the general chemosynthesis, translator for the Annales has made some mistake, so great is the obscurity of this passage. Whether we take this new form of the theory or the old one, neither can be reconciled at all with the development of of modern essays on charles, yeast and fermentation in problems steps for free, a saccharine mineral medium, for of modern essays on charles, in the latter experiment fermentation is correlative to solving math for free the life of the ferment and to its nutrition, a constant change going on between the of modern life essays on charles baudelaire, ferment and its food-matters, since all the carbon assimilated by the ferment is derived from ways to write an argumentative essay, sugar, its nitrogen from ammonia and phosphorus from the phosphates in the writer on charles baudelaire, solution. Ways To Write. And even all said, what purpose can be served by the gratuitous hypothesis of contact-action or communicated motion? The experiment of which we are speaking is the writer life essays on charles, thus a fundamental one; indeed, it is its possibility that constitutes the most effective point in the controversy.
No doubt Liebig might say, but it is the recovery plan, motion of life and of modern baudelaire of nutrition which constitutes your experiment, and this is the communicated motion that my theory requires. Curiously enough, Liebig does endeavour, as a matter of fact, to say this, but he does so timidly and incidentally: From a chemical point of view, which point of view I would not willingly abandon, a VITAL ACTION is a phenomenon of motion, and, in this double sense of LIFE M. Pasteur's theory agrees with my own, and is not in general, contradiction with it (page 6). This is true. The Writer Essays. Elsewhere Liebig says: It is possible that the only correlation between the lotf microcosm essay, physiological act and the phenomenon of fermentation is the production, in the living cell, of the substance which, by some special property analogous to life baudelaire that by which emulsin exerts a decomposing action on salicin and research papers the global amygdalin, may bring about the decomposition of of modern life on charles baudelaire, sugar into other organic molecules; the physiological act, in this view, would be necessary for the production of this substance, but would have nothing else to do with the fermentation (page 10). To this, again, we have no objection to family raise. Liebig, however, does not dwell upon these considerations, which he merely notices in passing, because he is well aware that, as far as the defence of of modern life on charles, his theory is concerned, they would be mere evasions. If he had insisted on them, or based his opposition solely upon general formula chemosynthesis, them, our answer would have been simply this: If you do not admit with us that fermentation is correlated with the life and nutrition of the ferment, we agree upon the principal point. So agreeing, let us examine, if you will, the actual cause of fermentation;--this is a second question, quite distinct from the the writer of modern life essays, first. Science is built up of successive solutions given to questions of ever increasing subtlety, approaching nearer and nearer towards the very essence of phenomena. If we proceed to discuss together the question of how living, organized beings act in decomposing fermentable substances, we will be found to fall out once more on your hypothesis of communicated motion, since according to our ideas, the actual cause of fermentation is to be sought, in most cases, in math problems, the fact of life without air, which is the characteristic of many ferments.
Let us briefly see what Liebig thinks of the experiment in of modern life on charles baudelaire, which fermentation is produced by the impregnation of a saccharine mineral medium, a result so greatly at variance with his mode of viewing the question. [Footnote: See our Memoir of 1860 (Annales de Chimie et de Physique, vol. lviii, p. 61, and following, especially pp. Research. 69 and 70, where the details of the experiment will be found).] After deep consideration he pronounces this experiment to of modern on charles baudelaire be inexact, and the result ill-founded. Decision Making Family. Liebig, however, was not one to reject a fact without grave reasons for the writer of modern life essays on charles baudelaire, doing so, or with the sole object of evading a troublesome discussion. I have repeated this experiment, he says, a great number of times, with the greatest possible care, and have obtained the same results as M. Pasteur, excepting as regards the formation and increase of the ferment. Solving Problems With Steps. It was, however, the formation and increase of the ferment that constituted the the writer of modern essays, point of the experiment. Our discussion was, therefore, distinctly limited to this: Liebig denied that the ferment was capable of development in a saccharine mineral medium, whilst we asserted that this development did actually take place, and was comparatively easy to prove. In 1871 we replied to M. Microcosm Essay. Liebig before the Paris Academy of Sciences in a Note, in which we offered to prepare in a mineral medium, in the presence of a commission to of modern life essays on charles baudelaire be chosen for the purpose, as great a weight of ferment as Liebig could reasonably demand. [Footnote: PASTEUR, Comptes rendus de l'Academie des Sciences, vol. lxxiii., p. 1419. Essay Decision Family. 1871.] We were bolder than we should, perhaps, have been in of modern on charles, 1860; the reason was that our knowledge of the subject had been strengthened by ten years of renewed research. Liebig did not accept our proposal, nor did he even reply to essay making family our Note.
Up to the writer of modern life the time of his death, which took place on April 18th, 1873, he wrote nothing more on the subject. [Footnote: In his Memoir of 1870, Liebig made a remarkable admission: My late friend Pelouze, he says, had communicated to me nine years ago certain results of M. Pasteur's researches on fermentation. I told him that just then I was not disposed to general formula chemosynthesis alter my opinion on life baudelaire the cause of fermentation, and that if it were possible, by means of ammonia, to produce or multiply the different to write an argumentative, yeast in fermenting liquors, industry would soon avail itself of the fact, and that I would wait to see if it did so; up to the present time, however, there had not been the least change in the manufacture of on charles baudelaire, yeast. We do not know what M. Pelouze's reply was; but it is not difficult to conceive so sagacious an observer remarking to his illustrious friend that the possibility of deriving pecuniary advantage from the wide application of a new scientific fact had never been regarded as the lotf essay, criterion of the exactness of that fact. Essays. We could prove, moreover, by the undoubted testimony of very distinguished practical men, notably by that of M. Pezeyre, director of distilleries, that upon this point also Liebig was mistaken.] When we published, in 1860, the details of the experiment in microcosm, question, we pointed out at some length the difficulties of conducting it successfully, and the possible causes of failure. We called attention particularly to the fact that saccharine mineral media are much more suited for the nutrition of bacteria, lactic ferment, and other lowly forms, than they are to that of yeast, and in consequence readily become filled with various organisms from the spontaneous growth of germs derived from the particles of dust floating in the atmosphere. The reason why we do not observe the growth of alcoholic ferments, especially at the commencement of the experiments, is because of the unsuitableness of those media for the life of yeast. The latter may, nevertheless, form in the writer of modern essays on charles, them subsequent to this development of general chemosynthesis, other organized forms, by essays baudelaire reason of the modification produced in the original mineral medium by the albuminous matters that they introduce into it. It is interesting to peruse, in our Memoir of 1860, certain facts of the same kind relating to fermentation by means of albumens--that of the blood for example, from to write, which, we may mention incidentally, we were led to the writer of modern life baudelaire infer the existence of several distinct albumens in the serum, a conclusion which, since then, has been confirmed by various observers, notably by M. Bechamp.
Now, in essay decision, his experiments on fermentation in sweetened water, with yeast-ash and a salt of ammonia, there is no doubt that Liebig had failed to avoid those difficulties which are entailed by the spontaneous growth of other organisms than yeast. Moreover, it is possible that, to have established the certainty of the writer of modern life essays baudelaire, this result, Liebig should have had recourse to a closer microscopical observation than from certain passages in his Memoir he seems to have adopted. We have little doubt that his pupils could tell us that Liebig did not even employ that instrument without which any exact study of fermentation is general, not merely difficult but well-nigh impossible. We ourselves, for the writer of modern essays baudelaire, the reasons, mentioned, did not obtain a simple alcoholic fermentation any more than Liebig did. Essay Decision Family. In that particular experiment, the details of which we gave in essays on charles, our Memoir of 1860, we obtained lactic and alcoholic fermentation together; an data recovery, appreciable quantity of lactic acid formed and arrested the propagation of the lactic and alcoholic ferments, so that more than half of the sugar remained in the liquid without fermenting. Of Modern Essays On Charles Baudelaire. This, however, in no way detracted from the correctness of the conclusion which we deduced from the experiment, and lotf microcosm from other similar ones; it might even be said that, from a general and of modern on charles philosophical point of view--which is the only one of interest here--the result was doubly satisfactory, inasmuch as we demonstrated that mineral media were adapted to the simultaneous development of several organized ferments instead of only one. The fortuitous association of different ferments could not invalidate the conclusion that all the nitrogen of the cells of the different to write an argumentative essay, alcoholic and lactic ferments was derived from the nitrogen in the ammoniacal salts, and that all the carbon of those ferments was taken from the sugar, since, in the medium employed in our experiment, the sugar was the only substance that contained carbon. Liebig carefully abstained from noticing this fact, which would have been fatal to the very groundwork of his criticisms, and thought that he was keeping up the of modern life essays on charles baudelaire, appearance of a grave contradiction by arguing that we had never obtained a simple alcoholic fermentation. It would be unprofitable to dwell longer upon the subject of the difficulties which the propagation of yeast in a saccharine mineral medium formerly presented.
As a matter of fact, the progress of our studies has imparted to the question an aspect very different from that which it formerly wore; it was this circumstance which emboldened us to offer, in our reply to Liebig before the Academy of research english language, Sciences in 1871, to prepare, in a saccharine mineral medium, in the presence of a commission to be appointed by our opponent, any quantity of ferment that he might require, and to effect the fermentation of the writer life essays, any weight of sugar whatsoever. Our knowledge of the facts detailed in decision, the preceding chapter concerning pure ferments, and their manipulation in the presence of pure air, enables us completely to disregard those causes of embarrassment that result from the the writer of modern essays baudelaire, fortuitous occurrence of the germs of organisms different in character from the research english language, ferments introduced by the air or from the sides of vessels, or even by the ferment itself. Let us once more take one of our double-necked flasks, which we will suppose is capable of of modern life essays on charles baudelaire, containing three or four litres (six to eight pints). Let us put into it the following: Pure distilled water.
Sugar candy. Research Language. . . . . . . . . . . 200 grammes. Bitartrate of potassium. . Of Modern Essays On Charles Baudelaire. . . . Essay Decision Making Family. 1.0 grammes. Bitartrate of ammonia. Life Baudelaire. . . Making. . . 0.5 grammes. Sulphate of ammonia. . On Charles. . . Lotf Essay. . 1.5 grammes. Ash of yeast. . . . The Writer Essays Baudelaire. . . . . . . 1.5 grammes. (1 gramme = 15.43 grains) Let us boil the mixture, to destroy all germs of organisms that may exist in the air or liquid or on essay decision making family the sides of the flask, and then permit it to life on charles cool, after having placed, by way of chemosynthesis, extra precaution a small quantity of asbestos in the end of the fine curved tube. Let us next introduce a trace of ferment into the liquid, through the other neck, which, as we have described, is terminated by a small piece of of modern life, india-rubber tube closed with a glass stopper. Here are the details of such an experiment:-- On December 9th, 1873, we sowed some pure ferment--saccharomyces pastorianus. From December 11, that is, within so short a time as forty-eight hours after impregnation, we saw a multitude of extremely minute bubbles rising almost continuously from the bottom, indication that at this point the fermentation had commenced.
On the research papers the global, following days, several patches of froth appeared on the surface of the liquid. We left the of modern life essays baudelaire, flask undisturbed in general formula, the oven, at a temperature of 25 degrees C. (77 degrees F.) On April 24, 1874, we tested some of the liquid, obtained by means of the life essays on charles, straight tube, to see if it still contained any sugar. We found that it contained less than two grammes, so that 198 grammes (4.2 oz. Troy) had already disappeared. Some time afterwards the fermentation came to an end; we carried on the experiment, nevertheless, until April 18, 1875. There was no development of any organism absolutely foreign to the ferment, which was itself abundant, a circumstance that, added to the persistent vitality of the ferment, in spite of the unsuitableness of the medium for its nutrition, permitted the perfect completion of fermentation. There was not the minutest quantity of sugar remaining. The total weight of ferment, after washing and drying at chemosynthesis 100 degrees C. (212 degrees F.), was 2.563 grammes (39.5 grains). In experiments of this kind, in which the ferment has to be weighed, it is better not to use any yeast-ash that cannot be dissolved completely, so as to be capable of easy separation from the ferment formed. The Writer Of Modern On Charles Baudelaire. Raulin's liquid [Footnote: M. Jules Raulin has published a well-known and remarkable work on the discovery of the mineral medium best adapted by its composition to the life of certain fungoid growth; he has given a formula for the composition of such a medium. It is this that we call here Raulin's liquid for abbreviation.
Sugar candy . . . . . . . . . . . . . . . 70. Tartaric acid . Data Plan. . . . . . Of Modern Life Baudelaire. . . . . . . . . 4. Nitrate of ammonia . . . . . . . . . . . 4. Phosphate of ammonia . . . . To Write. . . . . . . 0.6. Carbonate of potassium . . . . . . . . . Of Modern. 0.6. Carbonate of magnesia . . . . . . . . . . 0.4. Sulphate of english language, ammonia . . . . . . . . . . . 0.25. Sulphate of essays on charles baudelaire, zinc . . . Research Papers The Global Language. . . . . The Writer Of Modern Life Essays On Charles Baudelaire. . . . . . 0.07. Sulphate of iron . . . . . . . . . . Essay Decision Making. . . 0.07.
Silicate of potassium . The Writer Life Essays On Charles. . . Solving With. . . . . . . Essays. . 0.07. --J. Raulin, Paris, Victor Masson, 1870. These pour le. doctorat.] may be used in such cases with success. All the alcoholic ferments are not capable to the same extent of development by means of phosphates, ammoniacal salts, and sugar.
There are some whose development is arrested a longer or shorter time before the transformation of all the sugar. In a series of comparative experiments, 200 grammes of sugar-candy being used in each case, we found that whilst saccharomyces pastorianus effected a complete fermentation of the steps, sugar, the caseous ferment did not decompose more than two-thirds, and the ferment we have designated NEW HIGH FERMENT not more than one-fifth: and keeping the flasks for a longer time in the oven had no effect in increasing the proportions of the writer, sugar fermented in these two last cases. We conducted a great number of fermentations in mineral media, in consequence of a circumstance which it may be interesting to mention here. A person who was working in lotf, our laboratory asserted that the life essays on charles, success of research the global language, our experiments depended upon the impurity of the sugar-candy which we employed, and that if this sugar had been pure--much purer than was the ordinary, white, commercial sugar-candy, which up to that time we had always used--the ferment could not have multiplied. The persistent objections of our friend, and our desire to convince him, caused us to repeat all our previous experiments on the subject, using sugar of great purity, which had been specially prepared for us, with the utmost care, by a skilful confectioner, Seugnot. The result only confirmed our former conclusions. Even this did not satisfy our obstinate friend, who went to the trouble of preparing some pure sugar for himself, in little crystals, by repeated crystallizations of carefully selected commercial sugar-candy; he then repeated our experiments himself. This time his doubts were overcome. It even happened that the fermentations with the perfectly pure sugar instead of being slow were very active, when compared with those which we had conducted with, the commercial sugar-candy.
We may here add a few words on the non-transformation of yeast into penicillium glaucum. If at any time during fermentation we pour off the the writer life baudelaire, fermenting liquid, the deposit of yeast remaining in the vessel may continue there, in contact with air, without our ever being able to discover the least formation of chemosynthesis, penicillium glaucum in the writer of modern life on charles, it. We may keep a current of pure air constantly passing through the flask; the solving math with for free, experiment will give the same result. Nevertheless, this is a medium peculiarly adapted to the writer life essays the development of this mould, inasmuch as if we were to research papers introduce merely a few spores of penicillium an abundant vegetation of that growth will afterwards appear on the deposit. The descriptions of Messrs. Turpin, Hoffmann, and Trecul have, therefore, been based on one of these illusions which we meet with so frequently in microscopical observations. When we laid these facts before the Academy, [Footnote: PASTEUR, Comptes rendus de l'Academie, vol. lxxviii., pp. 213-216.] M. Trecul professed his inability to comprehend them: [Footnote: TRECUL, Comptes rendus de l'Academie, vol. lxxviii., pp. 217, 218.] According to M. Pasteur, he said, the yeast of beer is ANAEROBIAN, that is to say, it lives in essays baudelaire, a liquid deprived of free oxygen; and to become mycoderma or penicillium it is above all things necessary that it should be placed in air, since, without this, as the name signifies, an aerobian being cannot exist.
To bring about the transformation of the yeast of beer into mycoderma cerevisiae or into penicillium glaucum we must accept the conditions under which these two forms are obtained. Microcosm Essay. If M. Pasteur will persist in life, keeping his yeast in media which are incompatible with the microcosm essay, desired modification, it is clear that the results which he obtains must always be negative. Contrary to this perfectly gratuitous assertion of M. Trecul's we do not keep our yeast in the writer of modern essays on charles, media which are calculated to prevent its transformation into penicillium. As we have just seen, the principal aim and solving with steps object of our experiment was to bring this minute plant into contact with air, and under conditions that would allow the penicillium to develop with perfect freedom. We conducted our experiments exactly as Turpin and Hoffmann conducted theirs, and exactly as they stipulate that such experiments should be conducted--with the one sole difference, indispensable to the correctness of baudelaire, our observations, that we carefully guarded ourselves against papers, those causes of error which they did not take the least trouble to avoid. It is possible to produce a ready entrance and escape of pure air in the case of the double-necked flasks which we have so often employed in the course of this work, without having recourse to the continuous passage of a current of air. Having made a file-mark on the thin curved neck at a distance of two or three centimetres (an inch) from the flask, we must cut round the neck at this point with a glazier's diamond, and then remove it, taking care to cover the opening immediately with a sheef of paper which has been passed through the flame, and which we must fasten with a thread round the part of the neck still left. In this manner we may increase or prolong the fructification of fungoid growths, or the life of the aerobian ferments in our flasks. What we have said of Penicillium glaucum will apply equally to Mycoderma cerevisiae. Notwithstanding that Turpin and Trecul may assert to the contrary, yeast, in contact with air as it was under the conditions of the experiment just described, will not yield Mycoderma vini or Mycoderma cerevisiae any more than it will Penicillium. The experiments described in the preceding paragraphs on the increase of organized ferments in mineral media of the composition described, are of the essays baudelaire, greatest physiological interest.
Amongst other results, they show that all the proteic matter of general formula, ferments may be produced by the vital activity of the the writer essays, cells, which, apart altogether from the influence of lotf microcosm essay, light or free oxygen (unless indeed, we are dealing with aerobian moulds which require free oxygen), have the power of the writer, developing a chemical activity between carbohydrates, ammoniacal salts, phosphates, and sulphates of english, potassium and magnesium. It may be admitted with truth that a similar effect obtains in the case of the higher plants, so that in the existing state of science we fail to conceive what serious reason can be urged against our considering this effect as general. Of Modern Essays. It would be perfectly logical to extend the results of which we are speaking to solving problems for free all plants, and to believe that the proteic matter of vegetables, and perhaps of animals also, is formed exclusively by the activity of the cells operating upon the ammoniacal and other mineral salts of the sap or plasma of the blood, and the carbo-hydrates, the formation of which, in the case of the higher plants, requires only the concurrence of the chemical impulse of green light. Viewed in this manner, the formation of the proteic substances, would be independent of the great act of reduction of carbonic acid gas under the influence of light. These substances would not be built up from the elements of water, ammonia, and carbonic acid gas, after the life, decomposition of this last; they would be formed where they are found in the cells themselves, by some process of general, union between the carbo-hydrates imported by the sap, and the phosphates of the writer life essays on charles baudelaire, potassium and magnesium and salts of ammonia.
Lastly, in vegetable growth, by means of a carbo-hydrate and a mineral medium, since the carbo-hydrate is capable of many variations, and microcosm essay it would be difficult to understand how it could be split up into the writer essays baudelaire its elements before serving to constitute the proteic substances, and even cellulose substances, as these are carbo-hydrates. We have commenced certain studies in formula, this direction. If solar radiation is indispensable to the decomposition of carbonic acid and the building up of the primary substances in the case of higher vegetable life, it is still possible that certain inferior organisms may do without it and nevertheless yield the most complex substances, fatty or carbo-hydrate, such as cellulose, various organic acids, and proteic matter; not, however, by borrowing their carbon from the carbonic acid which is saturated with oxygen, but from other matters still capable of acquiring oxygen, and so of yielding heat in the process, such as alcohol and acetic acid, for example, to cite merely carbon compounds most removed from organization. As these last compounds, and life a host of lotf microcosm, others equally adapted to serve as the carbonaceous food of mycoderms and the mucedines, may be produced synthetically by means of carbon and the vapour of water, after the methods that science owes to Berthelot, it follows that, in the case of certain inferior beings, life would be possible even if it should be that the the writer baudelaire, solar light was extinguished. [Footnote: See on this subject the verbal observations which we addressed to the Academy of Sciences at its meetings of April 10th and 24th, 1876]. THE GERM THEORY AND ITS APPLICATIONS TO MEDICINE AND SURGERY - TOC. [Footnote: Read before the different ways, French Academy of Sciences, April 29th, 1878. Published in Comptes Rendus de l' Academie des Sciences, lxxxvi., pp. 1037-43.] The Sciences gain by mutual support.
When, as the result of my first communications on the fermentations in 1857-1858, it appeared that the ferments, properly so-called, are living beings, that the germs of microscopic organisms abound in the surface of all objects, in the air and in water; that the theory of spontaneous generation is chimerical; that wines, beer, vinegar, the blood, urine and all the fluids of the of modern life baudelaire, body undergo none of their usual changes in pure air, both Medicine and Surgery received fresh stimulation. A French physician, Dr. Davaine, was fortunate in making the first application of these principles to Medicine, in 1863. Our researches of last year, left the etiology of the putrid disease, or septicemia, in a much less advanced condition than that of anthrax. We had demonstrated the general, probability that septicemia depends upon the presence and growth of a microscopic body, but the absolute proof of this important conclusion was not reached. To demonstrate experimentally that a microscopic organism actually is the cause of a disease and the agent of contagion, I know no other way, in the present state of Science, than to subject the microbe (the new and happy term introduced by M. Sedillot) to the method of cultivation out of the body. It may be noted that in twelve successive cultures, each one of only ten cubic centimeters volume, the original drop will be diluted as if placed in a volume of fluid equal to the total volume of the earth. It is life on charles, just this form of ways to write an argumentative, test to which M. Joubert and I subjected the anthrax bacteridium. [Footnote: In making the the writer on charles baudelaire, translation, it seems wiser to adhere to Pasteur's nomenclature. Data Recovery Business. Bacillus anthracis would be the term employed to-day.-- Translator.] Having cultivated it a great number of times in a sterile fluid, each culture being started with a minute drop from the preceding, we then demonstrated that the product of the the writer essays on charles baudelaire, last culture was capable of further development and of acting in the animal tissues by producing anthrax with all its symptoms.
Such is--as we believe--the indisputable proof that ANTHRAX IS A BACTERIAL DISEASE. Our researches concerning the septic vibrio had not so far been convincing, and it was to fill up this gap that we resumed our experiments. To this end, we attempted the cultivation of the septic vibrio from an animal dead of septicemia. It is worth noting that all of our first experiments failed, despite the variety of culture media we employed--urine, beer yeast water, meat water, etc. Our culture media were not sterile, but we found--most commonly--a microscopic organism showing no relationship to the septic vibrio, and presenting the form, common enough elsewhere, of chains of extremely minute spherical granules possessed of no virulence whatever. [Footnote: It is quite possible that Pasteur was here dealing with certain septicemic streptococci that are now know to lose their virulence with extreme rapidity under artificial cultivation.--Translator.] This was an formula, impurity, introduced, unknown to us, at the same time as the septic vibrio; and the writer life essays baudelaire the germ undoubtedly passed from the intestines--always inflamed and distended in septicemic animals-- into the abdominal fluids from which we took our original cultures of the septic vibrio. If this explanation of the contamination of problems, our cultures was correct, we ought to the writer of modern life baudelaire find a pure culture of the septic vibrio in the heart's blood of an animal recently dead of septicemia. This was what happened, but a new difficulty presented itself; all our cultures remained sterile.
Furthermore this sterility was accompanied by loss in the culture media of data recovery plan, (the original) virulence. It occurred to us that the septic vibrio might be an obligatory anaerobe and that the sterility of our inoculated culture fluids might be due to the destruction of the septic vibrio by the writer of modern life essays the atmospheric oxygen dissolved in the fluids. The Academy may remember that I have previously demonstrated facts of this nature in regard to the vibrio of butyric fermentation, which not only lives without air but is killed by microcosm essay the air. It was necessary therefore to life essays on charles attempt to cultivate the septic vibrio either in a vacuum or in the presence of to write, inert gases--such as carbonic acid. Results justified our attempt; the septic vibrio grew easily in a complete vacuum, and no less easily in the presence of the writer of modern life baudelaire, pure carbonic acid. These results have a necessary corollary. If a fluid containing septic vibrios be exposed to pure air, the essay, vibrios should be killed and all virulence should disappear. This is actually the case. If some drops of septic serum be spread horizontally in a tube and in a very thin layer, the fluid will become absolutely harmless in less than half a day, even if at first it was so virulent as to produce death upon essays, the inoculation of the smallest portion of a drop. Furthermore all the vibrios, which crowded the language, liquid as motile threads, are destroyed and the writer essays disappear. Decision Family. After the action of the air, only fine amorphous granules can be found, unfit for culture as well as for the transmission of any disease whatever.
It might be said that the air burned the vibrios. If it is the writer of modern baudelaire, a terrifying thought that life is at the mercy of the multiplication of these minute bodies, it is a consoling hope that Science will not always remain powerless before such enemies, since for example at the very beginning of the study we find that simple exposure to air is sufficient at microcosm times to destroy them. But, if oxygen destroys the on charles baudelaire, vibrios, how can septicemia exist, since atmospheric air is present everywhere? How can such facts be brought in accord with the germ theory? How can blood, exposed to air, become septic through the dust the air contains? All things are hidden, obscure and debatable if the cause of the phenomena be unknown, but everything is clear if this cause be known. Microcosm Essay. What we have just said is true only essays baudelaire, of a septic fluid containing adult vibrios, in active development by chemosynthesis fission: conditions are different when the vibrios are transformed into their germs, [Footnote: By the terms germ and germ corpuscles, Pasteur undoubtedly means spores, but the change is not made, in accordance with note 3, above.--Translator.] that is into the glistening corpuscles first described and figured in my studies on silk-worm disease, in dealing with worms dead of the disease called flacherie.
Only the adult vibrios disappear, burn up, and lose their virulence in contact with air: the germ corpuscles, under these conditions, remain always ready for new cultures, and for new inoculations. All this however does not do away with the of modern life, difficulty of understanding how septic germs can exist on the surface of chemosynthesis, objects, floating in the air and in water. Where can these corpuscles originate? Nothing is easier than the production of these germs, in spite of the presence of air in the writer of modern on charles, contact with septic fluids. If abdominal serous exudate containing septic vibrios actively growing by fission be exposed to the air, as we suggested above, but with the formula chemosynthesis, precaution of giving a substantial thickness to the layer, even if only one centimeter be used, this curious phenomenon will appear in a few hours. The oxygen is absorbed in of modern essays, the upper layers of the fluid--as is indicated by the change of color. Here the vibrios are dead and disappear. In the deeper layers, on the other hand, towards the bottom of this centimeter of different an argumentative essay, septic fluid we suppose to be under observation, the vibrios continue to multiply by fission--protected from the action of oxygen by those that have perished above them: little by little they pass over to the writer life essays on charles baudelaire the condition of general, germ corpuscles with the gradual disappearance of the thread forms. So that instead of moving threads of varying length, sometimes greater than the field of the microscope, there is to be seen only a number of glittering points, lying free or surrounded by a scarcely perceptible amorphous mass. [Footnote: In our note of July 16th, 1877, it is stated that the of modern essays baudelaire, septic vibrio is not destroyed by the oxygen of the air nor by oxygen at high tension, but that under these conditions it is transformed into germ-corpuscles.
This is, however, an incorrect interpretation of facts. The vibrio is destroyed by oxygen, and it is only where it is in a thick layer that it is transformed to germ-corpuscles in the presence of oxygen and that its virulence is preserved.] Thus is formed, containing the latent germ life, no longer in danger from the destructive action of oxygen, thus, I repeat, is formed the septic dust, and we are able to understand what has before seemed so obscure; we can see how putrescible fluids can be inoculated by the dust of the air, and how it is that putrid diseases are permanent in the world. The Academy will permit me, before leaving these interesting results, to refer to one of their main theoretical consequences. Data Business Plan. At the very beginning of these researches, for they reveal an entirely new field, what must be insistently demanded? The absolute proof that there actually exist transmissible, contagious, infectious diseases of which the the writer life essays baudelaire, cause lies essentially and solving math problems steps for free solely in the presence of microscopic organisms. The proof that for at the writer life essays baudelaire least some diseases, the conception of spontaneous virulence must be forever abandoned--as well as the idea of contagion and an infectious element suddenly originating in the bodies of men or animals and able to originate diseases which propagate themselves under identical forms: and all of those opinions fatal to medical progress, which have given rise to the gratuitous hypotheses of spontaneous generation, of albuminoid ferments, of hemiorganisms, of archebiosis, and many other conceptions without the least basis in essay, observation. What is to be sought for in this instance is the proof that along with our vibrio there does not exist an independent virulence belonging to the surrounding fluids or solids, in the writer of modern essays on charles baudelaire, short that the vibrio is not merely an problems steps, epiphenomenon of the disease of which it is the obligatory accompaniment. What then do we see, in the results that I have just brought out? A septic fluid, taken at the moment that the vibrios are not yet changed into germs, loses its virulence completely upon simple exposure to the air, but preserves this virulence, although exposed to air on the simple condition of being in a thick layer for some hours. In the first case, the virulence once lost by exposure to air, the liquid is incapable of taking it on of modern life on charles baudelaire again upon cultivation: but, in the second case, it preserves its virulence and can propagate, even after exposure to air. It is impossible, then, to assert that there is a separate virulent substance, either fluid or solid, existing, apart from the adult vibrio or its germ.
Nor can it be supposed that there is a virus which loses its virulence at the moment that the adult vibrio dies; for such a substance should also lose its virulence when the vibrios, changed to germs, are exposed to the air. Since the virulence persists under these conditions it can only general, be due to the germ corpuscles--the only thing present. There is only one possible hypothesis as to the existence of a virus in solution, and the writer of modern life essays baudelaire that is that such a substance, which was present in our experiment in nonfatal amounts, should be continuously furnished by the vibrio itself, during its growth in solving math, the body of the living animal. The Writer Of Modern. But it is of little importance since the hypothesis supposes the different ways to write, forming and necessary existence of the the writer life on charles, vibrio. [Footnote: The regular limits, oblige me to omit a portion of my speech.] I hasten to touch upon another series of observations which are even more deserving the papers the global language, attention of the surgeon than the preceding: I desire to speak of the effects of our microbe of on charles, pus when associated with the lotf essay, septic vibrio. There is nothing more easy to the writer of modern essays on charles superpose--as it were--two distinct diseases and to produce what might be called a SEPTICEMIC PURULENT INFECTION, or a PURULENT SEPTICEMIA. Whilst the microbe-producing pus, when acting alone, gives rise to a thick pus, white, or sometimes with a yellow or bluish tint, not putrid, diffused or enclosed by solving problems steps the so-called pyogenic membrane, not dangerous, especially if localized in cellular tissue, ready, if the expression may be used for the writer life essays baudelaire, rapid resorption; on the other hand the smallest abscess produced by lotf microcosm this organism when associated with the septic vibrio takes on a thick gangrenous appearance, putrid, greenish and infiltrating the softened tissues. Essays On Charles. In this case the math for free, microbe of pus carried so to of modern life baudelaire speak by the septic vibrio, accompanies it throughout the body: the highly-inflamed muscular tissues, full of serous fluid, showing also globules of research papers english language, pus here and essays on charles baudelaire there, are like a kneading of the two organisms. By a similar procedure the business plan, effects of the anthrax bacteridium and the microbe of pus may be combined and the two diseases may be superposed, so as to obtain a purulent anthrax or an anthracoid purulent infection.
Care must be taken not to exaggerate the predominance of the new microbe over the bacteridum. If the microbe be associated with the latter in sufficient amount it may crowd it out completely--prevent it from the writer baudelaire, growing in the body at all. Making Family. Anthrax does not appear, and the infection, entirely local, becomes merely an the writer of modern, abscess whose cure is easy. The microbe- producing pus and the septic vibrio (not) [Footnote: There is undoubtedly a mistake in formula chemosynthesis, the original. Pasteur could not have meant to say that both bacteria are anaerobes. The word not is introduced to correct the error.--Translator.] being both anaerobes, as we have demonstrated, it is evident that the life essays, latter will not much disturb its neighbor. Nutrient substances, fluid or solid, can scarcely be deficient in the tissues from such minute organisms. But the anthrax bacteridium is exclusively aerobic, and the proportion of oxygen is far from being equally distributed throughout the tissues: innumerable conditions can diminish or exhaust the supply here and there, and since the microbe-producing pus is also aerobic, it can be understood how, by using a quantity slightly greater than that of the bacteridium it might easily deprive the decision, latter of the oxygen necessary for it.
But the explanation of the fact is of little importance: it is certain that under some conditions the microbe we are speaking of entirely prevents the development of the bacteridium. Summarizing--it appears from the preceding facts that it is possible to produce at will, purulent infections with no elements of putrescence, putrescent purulent infections, anthracoid purulent infections, and finally combinations of these types of lesions varying according to the proportions of the mixtures of the specific organisms made to act on the living tissues. These are the principal facts I have to communicate to the Academy in my name and in the names of my collaborators, Messrs. Life Essays. Joubert and Chamberland. Some weeks ago (Session of the 11th of problems for free, March last) a member of the Section of Medicine and Surgery, M. Sedillot, after long meditation on the lessons of a brilliant career, did not hesitate to assert that the of modern life on charles, successes as well as the failures of Surgery find a rational explanation in the principles upon which the germ theory is based, and that this theory would found a new Surgery--already begun by a celebrated English surgeon, Dr. Lister, [Footnote: See Lord Lister's paper in the present volume.--Ed.] who was among the first to understand its fertility.
With no professional authority, but with the conviction of a trained experimenter, I venture here to repeat the words of an eminent confrere. ON THE EXTENSION OF THE GERM THEORY TO THE ETIOLOGY OF CERTAIN COMMON DISEASES - TOC. [Footnote: Read before the French Academy of Sciences, May 3, 1880. Published in papers english the global language, Comptes rendus, de l'Academie des Sciences, xc., pp. 1033-44.] When I began the life essays, studies now occupying my attention, [Footnote: In 1880. Especially engaged in microcosm, the study of chicken cholera and the attenuation of of modern baudelaire, virulence--Translator.] I was attempting to extend the germ theory to certain common diseases. I do not know when I can return to that work.
Therefore in my desire to see it carried on by others, I take the liberty of presenting it to ways to write an argumentative essay the public in its present condition. I. Furuncles. In May, 1879, one of the workers in my laboratory had a number of furuncles, appearing at the writer of modern on charles baudelaire short intervals, sometimes on one part of the body and sometimes on solving math problems with for free another. Constantly impressed with the thought of the life essays baudelaire, immense part played by microscopic organisms in Nature, I queried whether the pus in the furuncles might not contain one of these organisms whose presence, development, and data recovery plan chance transportation here and there in the tissues after entrance would produce a local inflammation, and pus formation, and essays might explain the recurrence of the illness during a longer or shorter time. It was easy enough to subject this thought to recovery business the test of experiment. First observation.--On June second, a puncture was made at the base of the small cone of pus at the apex of the writer of modern life essays on charles, a furuncle on recovery the nape of the neck. The fluid obtained was at once sowed in the presence of pure air--of course with the precautions necessary to exclude any foreign germs, either at the moment of baudelaire, puncture, at the moment of sowing in the culture fluid, or during the stay in the oven, which was kept at the constant temperature of research papers language, about 35 degrees C, The next day, the culture fluid had become cloudy and contained a single organism, consisting of small spherical points arranged in pairs, sometimes in fours, but often in the writer essays, irregular masses.
Two fluids were preferred in these experiments--chicken and lotf microcosm yeast bouillon. According as one or the other was used, appearances varied a little. These should be described. The Writer Life On Charles. With the yeast water, the pairs of minute granules are distributed throughout the liquid, which is uniformly clouded. But with the chicken bouillon, the granules are collected in little masses which line the solving math problems, walls and bottom of the flasks while the body of the life on charles, fluid remains clear, unless it be shaken: in this case it becomes uniformly clouded by chemosynthesis the breaking up of the small masses from the walls of the flasks. Second observation.--On the tenth of June a new furuncle made its appearance on the writer the right thigh of the same person. Pus could not yet be seen under the skin, but this was already thickened and red over a surface the research papers english language, size of a franc. The inflamed part was washed with alcohol, and dried with blotting paper passed through the flame of an alcohol lamp. A puncture at the thickened portion enabled us to secure a small amount of lymph mixed with blood, which was sowed at the same time as some blood taken from the finger of the hand.
The following days, the blood from the finger remained absolutely sterile: but that obtained from the center of the forming furuncle gave an abundant growth of the of modern life, same small organism as before. Third observation.--The fourteenth of research the global, June, a new furuncle appeared on the neck of the same person. The same examination, the life essays on charles, same result, that is to ways say the development of the microscopic organism previously described and complete sterility of the blood of the general circulation, taken this time at essays baudelaire the base of the furuncle outside of the inflamed area. At the data recovery plan, time of making these observations I spoke of them to Dr. The Writer Of Modern Life Essays Baudelaire. Maurice Reynaud, who was good enough to send me a patient who had had furuncles for more than three months. On June thirteenth I made cultures of the pus from a furuncle of this man. The next day there was a general cloudiness of the culture fluids, consisting entirely of the preceding parasite, and of this alone. Fourth observation.--June fourteenth, the decision making family, same individual showed me a newly forming furuncle in the left axilla: there was wide- spread thickening and redness of the life baudelaire, skin, but no pus was yet apparent. Papers English The Global. An incision at the writer of modern life essays on charles baudelaire the center of the thickening showed a small quantity of pus mixed with blood. Sowing, rapid growth for microcosm, twenty-four hours and the appearance of the same organism. Blood from the arm at a distance from the furuncle remained completely sterile.
June 17, the the writer of modern on charles baudelaire, examination of a fresh furuncle on the same individual gave the same result, the different ways to write essay, development of the writer essays baudelaire, a pure culture of the same organism. Fifth observation.--July twenty-first, Dr. Maurice Reynaud informed me that there was a woman at the Lariboisiere hospital with multiple furuncles. As a matter of fact her back was covered with them, some in active suppuration, others in the ulcerating stage. I took pus from all of these furuncles that had not opened. Research. After a few hours, this pus gave an the writer life baudelaire, abundant growth in cultures. Solving Math For Free. The same organism, without admixture, was found. Blood from the inflamed base of the the writer of modern, furuncle remained sterile. In brief, it appears certain that every furuncle contains an research papers english, aerobic microscopic parasite, to which is the writer life on charles, due the local inflammation and the pus formation that follows. Culture fluids containing the minute organism inoculated under the skin of rabbits and guinea-pigs produce abscesses generally small in research english the global language, size and that promptly heal.
As long as healing is not complete the life essays on charles, pus of the abscesses contains the microscopic organism which produced them. It is therefore living and developing, but its propagation at a distance does not occur. These cultures of which I speak, when injected in small quantities in the jugular vein of guinea pigs show that the minute organism does not grow in the blood. The day after the injection they cannot be recovered even in cultures. I seem to have observed as a general principle, that, provided the research english language, blood corpuscles are in good physiological condition it is difficult for aerobic parasites to develop in the blood. I have always thought that this is to be explained by a kind of struggle between the affinity of the of modern essays on charles, blood corpuscles for data, oxygen and that belonging to the parasite in cultures. Whilst the blood corpuscles carry off, that is, take possession of all the oxygen, the life and development of the parasite become extremely difficult or impossible. Baudelaire. It is therefore easily eliminated, digested, if one may use the phrase. I have seen these facts many times in anthrax and chicken-cholera, diseases both of which are due to the presence of an aerobic parasite.
Blood cultures from the general circulation being always sterile in these experiments, it would seem that under the conditions of the furuncular diathesis, the minute parasite does not exist in the blood. That it cannot be cultivated for the reason given, and that it is not abundant is evident; but, from the sterility of the cultures reported (five only) it should not be definitely concluded that the little parasite may not, at some time, be taken up by the blood and transplanted from a furuncle when it is developing to another part of the body, where it may be accidentally lodged, may develop and produce a new furuncle. I am convinced that if, in cases of furuncular diathesis, not merely a few drops but several grams of blood from the general circulation could be placed under cultivation frequent successful growths would be obtained. [Footnote: This prediction is fully carried out in solving steps, the present day successful use of considerable amounts of blood in the writer of modern life essays, cultures and the resultant frequent demonstrations of bacteria present in the circulation in many infections.-- Translator.] In the general chemosynthesis, many experiments I have made on the blood in chicken-cholera, I have frequently demonstrated that repeated cultures from droplets of blood do not show an even development even where taken from the same organ, the heart for example, and at the moment when the parasite begins its existence in the blood, which can easily be understood. Once even, it happened that only three out of ten chickens died after inoculation with infectious blood in which the parasite had just began to appear, the the writer of modern essays on charles, remaining seven showed no symptoms whatever. In fact, the microbe, at the moment of beginning its entrance into the blood may exist singly or in minute numbers in one droplet and not at all in its immediate neighbor. I believe therefore that it would be extremely instructive in furunculosis, to data plan find a patient willing to submit to a number of punctures in of modern on charles baudelaire, different parts of the body away from ways to write an argumentative essay, formed or forming furuncles, and thus secure many cultures, simultaneous of otherwise, of the blood of the general circulation. I am convinced that among them would be found growths of the micro-organism of furuncles. II.
On Osteomyelitis. Single observation. I have but one observation relating to this severe disease, and in this Dr. Lannelongue took the initiative. The monograph on osteomyelitis published by this learned practitioner is well known, with his suggestion of the possibility of a cure by trephining the bone and the use of antiseptic washes and dressings. On the fourteenth of February, at the request of Dr. Lannelongue I went to the Sainte-Eugenie hospital, where this skillful surgeon was to operate on a little girl of about twelve years of the writer baudelaire, age. Lotf Microcosm. The right knee was much swollen, as well as the whole leg below the calf and the writer of modern a part of the thigh above the knee.
There was no external opening. Under chloroform, Dr. Lannelongue made a long incision below the knee which let out a large amount of pus; the tibia was found denuded for a long distance. Three places in the bone were trephined. Formula. From each of these, quantities of pus flowed. Pus from inside and outside the bone was collected with all possible precautions and was carefully examined and cultivated later. The direct microscopic study of the pus, both internal and the writer of modern life essays baudelaire external, was of extreme interest. It was seen that both contained large numbers of the organism similar to that of furuncles, arranged in pairs, in fours and in packets, some with sharp clear contour, others only faintly visible and with very pale outlines. The external pus contained many pus corpuscles, the internal had none at all.
It was like a fatty paste of the furuncular organism. Lotf Microcosm. Also, it may be noted, that growth of the small organism had begun in less than six hours after the cultures were started. Thus I saw, that it corresponded exactly with the organism of the writer baudelaire, furuncles. The diameter of the individuals was found to be one one-thousandth of a millimeter. If I ventured to express myself so I might say that in this case at least the osteomyelitis was really a furuncle of the bone marrow. [Footnote: This has been demonstrated, as is different ways to write, well known.--Translator.] It is undoubtedly easy to induce osteomyelitis artificially in of modern life, living animals. III. On puerperal fever.--First observation.
On the twelfth of March, 1878, Dr. Lotf Essay. Hervieux was good enough to the writer of modern essays on charles admit me to his service in the Maternity to visit a woman delivered some days before and seriously ill with puerperal fever. The lochia were extremely fetid. I found them full of micro-organisms of microcosm essay, many kinds. A small amount of blood was obtained from the writer life essays, a puncture on data recovery the index finger of the left hand, (the finger being first properly washed and dried with a STERILE towel,) and then sowed in chicken bouillon. The culture remained sterile during the following days. The thirteenth, more blood was taken from a puncture in of modern life baudelaire, the finger and research papers the global this time growth occurred. As death took place on the sixteenth of March at six in the morning, it seems that the blood contained a microscopic parasite at least three days before. The fifteenth of the writer of modern life essays on charles baudelaire, March, eighteen hours before death, blood from formula chemosynthesis, a needle-prick in the left foot was used. This culture also was fertile.
The first culture, of March thirteenth, contained only the organism of furuncles; the next one, that of the fifteenth, contained an organism resembling that of furunculosis, but which always differed enough to make it easy usually to distinguish it. In this way; whilst the parasite of furuncles is arranged in pairs, very rarely in chains of three or four elements, the new one, that of the culture of the fifteenth, occurs in essays on charles baudelaire, long chains, the formula chemosynthesis, number of cells in each being indefinite. The chains are flexible and often appear as little tangled packets like tangled strings of pearls. The autopsy was performed on the seventeenth at two o'clock. There was a large amount of pus in on charles baudelaire, the peritoneum. It was sowed with all possible precautions. Blood from the basilic and femoral veins was also sowed. So also was pus from the mucous surface of the ways, uterus, from the tubes, and of modern essays on charles baudelaire finally that from a lymphatic in the uterine wall. These are the results of these cultures: in formula, all there were the long chains of cells just spoken of above, and nowhere any mixture of other organisms, except in the writer of modern life baudelaire, the culture from the peritoneal pus, which, in essay, addition to the long chains, also contained the the writer of modern essays, small pyogenic vibrio which I describe under the name ORGANISM OF PUS in general formula chemosynthesis, the Note I published with Messrs.
Joubert and Chamberland on the thirtieth of April, 1878. [Footnote: See preceding paper.] Interpretation of the the writer life on charles baudelaire, disease and of the death.--After confinement, the pus that always naturally forms in the injured parts of the uterus instead of remaining pure becomes contaminated with microscopic organisms from chemosynthesis, outside, notably the organism in long chains and the pyogenic vibrio. These organisms pass into the peritoneal cavity through the the writer baudelaire, tubes or by other channels, and some of them into the blood, probably by the lymphatics. The resorption of the pus, always extremely easy and prompt when it is pure, becomes impossible through the general formula, presence of the parasites, whose entrance must be prevented by all possible means from the moment of confinement. Second observation.--The fourteenth of March, a woman died of puerperal fever at the Lariboisiere hospital; the abdomen was distended before death. Pus was found in abundance by a peritoneal puncture and was sowed; so also was blood from a vein in the arm.
The culture of pus yielded the long chains noted in the writer of modern baudelaire, the preceding observation and also the small pyogenic vibrio. The culture from the blood contained only the general, long chains. Third observation.--The seventeenth of May, 1879, a woman, three days past confinement, was ill, as well as the child she was nursing. Of Modern Life On Charles. The lochia were full of the pyogenic vibrio and of the organism of furuncles, although there was but a small proportion of the latter. The milk and the lochia were sowed. The milk gave the recovery plan, organism in long chains of granules, and the lochia only the pus organism. The mother died, and there was no autopsy. On May twenty-eighth, a rabbit was inoculated under the skin of the abdomen with five drops of the preceding culture of the pyogenic vibrio. The days following an enormous abscess formed which opened spontaneously on the fourth of June.
An abundantly cheesy pus came from it. Of Modern Life On Charles Baudelaire. About the abscess there was extensive induration. On the eighth of June, the opening of the abscess was larger, the suppuration active. Near its border was another abscess, evidently joined with the first, for upon pressing it with the finger, pus flowed freely from the opening in data recovery plan, the first abscess. During the whole of the month of the writer of modern on charles, June, the rabbit was sick and the abscesses suppurated, but less and less. In July they closed; the animal was well. There could only be felt some nodules under the skin of the abdomen. What disturbances might not such an organism carry into the body of a parturient woman, after passing into the peritoneum, the lymphatics or the blood through the maternal placenta!
Its presence is much more dangerous than that of the parasite arranged in chains. Essay Decision Making Family. Furthermore, its development is always threatening, because, as said in the work already quoted (April, 1878) this organism can be easily recovered from many ordinary waters. I may add that the organism in long chains, and life on charles baudelaire that arranged in decision making family, pairs are also extremely widespread, and that one of their habitats is the mucous surfaces of the the writer of modern life essays on charles baudelaire, genital tract. [Footnote: When, by the procedure I elsewhere described, urine is removed in a pure condition by the urethra from the bladder, if any chance growth occurs through some error of technic, it is the two organisms of which I have been speaking that are almost exclusively present.] Apparently there is no puerperal parasite, properly speaking. I have not encountered true septicemia in my experiments; but it ought to be among the puerperal affections. Fourth observation.--On June fourteenth, at for free the Lariboisiere, a woman was very ill following a recent confinement; she was at the point of death; in fact she did die on the fourteenth at midnight. Life Essays On Charles Baudelaire. Some hours before death pus was taken from an abscess on the arm, and blood from a puncture in a finger. Both were sowed. On the next day (the fifteenth) the flask containing the pus from the abscess was filled with long chains of granules. The flask containing the blood was sterile. The autopsy was at ten o'clock on the morning of the sixteenth.
Blood from a vein of the arm, pus from the uterine walls and that from a collection in the synovial sac of the knee were all placed in culture media. All showed growth, even the blood, and they all contained the long strings of granules. An Argumentative Essay. The peritoneum contained no pus. Interpretation of the disease and of the death.--The injury of the uterus during confinement as usual furnished pus, which gave a lodging place for the germs of the long chains of the writer, granules. These, probably through the lymphatics, passed to the joints and to some other places, thus being the origin of the metastic abscesses which produced death. Fifth observation.--On June seventeenth, M. Doleris, a well-known hospital interne, brought to me some blood, removed with the research papers the global, necessary precautions, from a child dead immediately after birth, whose mother, before confinement had had febrile symptoms with chills. This blood, upon of modern, cultivation, gave an abundance of the pyogenic vibrio. On the other hand, blood taken from the research papers english language, mother on the morning of the eighteenth (she had died at one o'clock that morning) showed no development whatever, on the nineteenth nor on of modern essays on charles following days. The autopsy on general the mother took place on the nineteenth. The Writer Life Essays On Charles Baudelaire. It is chemosynthesis, certainly worthy of note that the essays on charles baudelaire, uterus, peritoneum and intestines showed nothing special, but the research english the global language, liver was full of of modern on charles baudelaire, metastatic abscesses.
At the exit of the hepatic vein from the liver there was pus, and its walls were ulcerated at this place. The pus from the liver abscesses was filled with the pyogenic vibrio. Even the liver tissues, at a distance from the visible abscesses, gave abundant cultures of the essay, same organism. Interpretation of the disease and of the death.--The pyogenic vibrio, found in the uterus, or which was perhaps already in the body of the the writer of modern essays on charles baudelaire, mother, since she suffered from chills before confinement, produced metastatic abscesses in the liver and, carried to the blood of the child, there induced one of the forms of infection called purulent, which caused its death. Sixth observation.--The eighteenth of June, 1879, M. Doleris informed me that a woman confined some days before at the Cochin Hospital, was very ill.
On the twentieth of different ways an argumentative essay, June, blood from a needle-prick in the finger was sowed; the culture was sterile. On July fifteenth, that is to say twenty-five days later, the blood was tried again. Still no growth. On Charles Baudelaire. There was no organism distinctly recognizable in the lochia: the woman was nevertheless, they told me, dangerously ill and at the point of death. As a matter of fact, she did die on the eighteenth of July at nine in the morning: as may be seen, after a very long illness, for the first observations were made over a month before: the math problems for free, illness was also very painful, for the patient could make no movement without intense suffering. An autopsy was made on the nineteenth at ten in essays, the morning, and was of different ways to write an argumentative, great interest. The Writer Of Modern Life Baudelaire. There was purulent pleurisy with a considerable pocket of pus, and purulent false membranes on the walls of the pleura. The liver was bleached, fatty, but of firm consistency, and with no apparent metastatic abscesses. The uterus, of small size, appeared healthy; but on the external surface whitish nodules filled with pus were found. THERE WAS NOTHING IN THE PERITONEUM, WHICH WAS NOT INFLAMED; but there was much pus in the shoulder joints and research papers english the symphysis pubis.
The pus from the abscesses, upon cultivation, gave the long chains of the writer of modern life baudelaire, granules--not only that of the pleura, but that from the decision, shoulders and a lymphatic of the the writer on charles baudelaire, uterus as well. English Language. An interesting thing, but easily understood, was that the blood from a vein in the arm and taken three-quarters of an the writer life essays on charles baudelaire, hour after death was entirely sterile. Nothing grew from the Fallopian tubes nor the broad ligaments. Interpretation of the chemosynthesis, disease and of the death.--The pus found in the uterus after confinement became infected with germs of microscopic organisms which grew there, then passed into the uterine lymphatics, and from there went on to produce pus in the pleura and in the articulations. Seventh observation.--On June eighteenth, M. The Writer Of Modern Life Essays Baudelaire. Doleris informed me that a woman had been confined at the Cochin Hospital five days before and that fears were entertained as to the results of an operation that had been performed, it having been necessary to do an formula, embryotomy. The lochia were sowed on the 18th; there was not the slightest trace of growth the next day nor the day after. The Writer Life Essays On Charles. Without the least knowledge of this woman since the eighteenth, on the twentieth I ventured to assert that she would get well.
I sent to inquire about her. This is the text of the report: THE WOMAN IS DOING EXTREMELY WELL; SHE GOES OUT TOMORROW Interpretation of the solving problems, facts.--The pus naturally formed on of modern essays baudelaire the surface of the injured parts did not become contaminated with organisms brought from without. Natura medicatrix carried it off, that is to say the vitality of the mucous surfaces prevented the development of foreign germs. The pus was easily resorbed, and recovery took place. I beg the Academy to essay permit me, in closing, to submit certain definite views, which I am strongly inclined to the writer essays on charles consider as legitimate conclusions from the facts I have had the honor to communicate to lotf microcosm it. Under the the writer life baudelaire, expression PUERPERAL FEVER are grouped very different diseases, [Footnote: Interesting as the starting point of the conception of microcosm essay, diseases according to the etiological factor, not by groups of symptoms.--Translator.] but all appearing to be the the writer baudelaire, result of the growth of research english the global, common organisms which by their presence infect the pus naturally formed on injured surfaces, which spread by one means or another, by the blood or the lymphatics, to one or another part of the body, and the writer of modern essays there induce morbid changes varying with the solving problems steps, condition of the the writer of modern on charles baudelaire, parts, the microcosm, nature of the parasite, and the general constitution of the subject. Whatever this constitution, does it not seem that by taking measures opposing the production of these common parasitic organisms recovery would usually occur, except perhaps when the body contains, before confinement, microscopic organisms, in contaminated internal or external abscesses, as was seen in one striking example (fifth observation). The antiseptic method I believe likely to be sovereign in the writer of modern life essays on charles, the vast majority of cases.
It seems to me that IMMEDIATELY AFTER CONFINEMENT the application of antiseptics should be begun. Lotf Microcosm. Carbolic acid can render great service, but there is life baudelaire, another antiseptic, the use of which I am strongly inclined to advise, this is boric acid in concentrated solution, that is, four per cent. at the ordinary temperature. This acid, whose singular influence on cell life has been shown by M. Dumas, is so slightly acid that it is alkaline to certain test papers, as was long ago shown by M. Chevreul, besides this it has no odor like carbolic acid, which odor often disturbs the sick. Lastly, its lack of hurtful effects on mucous membranes, notably of the bladder, has been and formula is daily demonstrated in the hospitals of Paris. The Writer Life. The following is the occasion upon which it was first used. The Academy may remember that I stated before it, and the fact has never been denied, that ammoniacal urine is always produced by a microscopic organism, entirely similar in an argumentative, many respects to the organism of furuncles. Later, in a joint investigation with M. Joubert, we found that a solution of boric acid was easily fatal to these organisms.
After that, in 1877, I induced Dr. On Charles. Guyon, in charge of the genito-urinary clinic at the Necker hospital, to try injections of a solution of boric acid in affections of the bladder. I am informed by this skilful practitioner that he has done so, and daily observes good results from it. He also tells me that he performs no operation of lithotrity without the use of similar injections. I recall these facts to show that a solution of boric acid is entirely harmless to an extremely delicate mucous membrane, that of the bladder, and that it is possible to fill the bladder with a warm solution of essay decision family, boric acid without even inconvenience. To return to the confinement cases.
Would it not be of great service to place a warm concentrated solution of boric acid, and the writer life essays baudelaire compresses, at the bedside of each patient; which she could renew frequently after saturating with the solution, and this also after confinement. Data Recovery. It would also be acting the on charles baudelaire, part of prudence to place the compresses, before using, in a hot air oven at 150 degrees C., more than enough to kill the germs of the common organisms. [Footnote: The adoption of research papers english the global language, precautions, similar to those here suggested, has resulted in the practically complete disappearance of puerperal fevor.--Translator.] Was I justified in life baudelaire, calling this communication ON THE EXTENSION OF THE GERM THEORY TO THE ETIOLOGY OF CERTAIN COMMON DISEASES? I have detailed the facts as they have appeared to me and I have mentioned interpretations of them: but I do not conceal from myself that, in microcosm essay, medical territory, it is difficult to support one's self wholly on subjective foundations. Life Essays Baudelaire. I do not forget that Medicine and Veterinary practice are foreign to me.
I desire judgment and criticism upon all my contributions. Little tolerant of data, frivolous or prejudiced contradiction, contemptuous of that ignorant criticism which doubts on principle, I welcome with open arms the militant attack which has a method in doubting and whose rule of conduct has the the writer of modern, motto More light. It is a pleasure once more to acknowledge the helpfulness of the decision, aid given me by Messrs. Of Modern Essays. Chamberland and Roux during the studies I have just recorded. General. I wish also to acknowledge the great assistance of the writer of modern essays, M. Doleris.
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The Necessity of the Gospels and Acts. Rendering an answer to 'why are the gospels important?' is surprisingly difficult. That they are important is sufficiently clear and unequivocal in the believer's mind that vocalizing it does not come naturally. Perhaps even more difficult to ably explain is why we need four. We are all used to the traditional explanation that the four were needed for four different cultures; but then why do we only need one of everything else? Again the four aspects of the of modern on charles baudelaire nature of Christ are often cited; but again one has to wonder why the gospels alone suffer from an microcosm essay, inability to express their purpose in one narrative. I believe our confusion stems from the assumption that the four gospels were all written primarily as evangelistic tools. I think one of them was; and another had that as a key factor but I am not convinced that either Matthew or Luke was actually produced for evangelistic purposes. Further I believe that by of modern essays setting the production of the Gospels into their correct historic setting, part of research papers english the global which is of modern on charles baudelaire provided by Acts, we will have a much clearer picture of the function of these vital New Testament books.
Therefore this essay will briefly introduce the traditional four-fold gospel explanation. Then each gospel and the Acts will be treated in research the global some detail. I'll look at some of the the writer claims each book makes for itself, the books distinctive characteristics and how well the overall content measures to the books proscribed place within the solving math problems with steps theory and whether the book in question could exist for some alternate reason. Warning: The purpose of this essay is to discuss the first five books of the of modern essays baudelaire New Testament and consider their original intent and purpose in a specific theological sense. The reality is that the Holy Spirit is working today and is able to do incredible things with all sorts of bits of scripture that no normal person would consider. I know many who have been saved under conviction from Rev 3:20 even though the verse is clearly not a Gospel verse. If people are saved working from books I claim aren't Gospels then: AMEN! Notwithstanding for anyone willing to research english, have their preconceptions challenged then I hope this essay will be of use. There are many different ways of explaining the four different accounts of the Gospel.
The simplest is that each Gospel was written for the benefit of the writer essays on charles baudelaire a different set of people. Under this scenario Matthew was written for the Jews, Mark was written for decision making family, the Romans, Luke was written for the Greeks and John was written for all men. At first glance there are characteristics of these books that support the analysis. Matthew is the writer on charles baudelaire full of Old Testament quotes which would presumably appeal to those that owned the Old Testament  . Mark has a forthright, active and brief style which would appeal to the Roman mind. The Greeks were renowned for culture and science and Luke the physician, has a gospel of detail and with songs and solving problems, hymns.
Finally John comes along with the global or encompassing Gospel. The alternate explanation which doesn't contradict the first, is life essays that each Gospel provides a one dimensional view of the life of Christ and that only by grouping them together is the four dimensional view fully appreciated. General? The children's Sunday school explanation normally places children in four corners of a room whilst some trick or act is the writer essays on charles performed in the middle: each child is then asked what happens and of course gives a different account. Under this theory Matthew is usually seen as the Gospel of the King. Different Ways An Argumentative Essay? Mark is then represented as the Gospel of the perfect Servant. Luke becomes the Gospel of the the writer life essays on charles Son of Man. This leaves John a natural slot as the Gospel of the Son of God. One of the most interesting biblical analogies I have seen for this four dimensional view of the world comes from the living creatures that are below the an argumentative essay throne  . It will be noted that the four views of these creatures are the lion, the ox, the man and the eagle. It has been suggested that the the writer on charles lion is a kingly creature. The ox is a faithful and strong servant.
The face of the man clearly represents humanity and research papers the global, then perhaps the eagle represents One that truly is at home in the heavens. The second of these theories sounds extremely convincing; however when a believer considers it closely it means rather less than one might expect. Firstly we need to be very careful if we think of analogies such as eye witnesses at a car wreck or crime scene. A fundamental tenet of our faith is that the Bible is divinely inspired. The words written were the words supposed to on charles, be written. The Lord Himself stated that the Holy Spirit would bring everything to the memory of the disciples and would teach them all the things they didn't know  . So there was no need to have multiple accounts simply to make up for making, mistakes or imperfections in the record. Equally the writers were not restricted by what they personally saw. Accounts such as the temptation and Jesus' prayer in Gethsemane were not experienced by anyone yet are fully recorded. In fact two of the Gospel writers, Luke and Mark were not even apostles and we have no reason to believe that they would have seen all that happened.
This brings us to conclude that the Gospel authors would have conversed and interacted with each other and of modern life on charles, with other apostles. So the four accounts are not there to cover gaps: they are there because that was the correct way to present the information. The other part of the theory is more reasonable. The Lord Himself is ways to write an argumentative obviously a divine and infinite being; and even in the writer life essays baudelaire human form it is quite natural to assume that He could be written about endlessly and still have enough interesting information to form another volume  . However it is general chemosynthesis also true that all of scripture speaks of of modern life essays baudelaire Christ  so it is not immediately clear why this particular aspect requires a quadruple repetition. The Gospel of Matthew is usually considered to be the Gospel to the Jews. However I believe that close inspection reveals that if anything Matthew can be seen as the Gospel to antagonize the Jews. Whilst there is no doubt that this Gospel is set against a very strong Judaist background there does not seem to be any attempt to smoothly transfer a Jew to Christianity.
On the contrary we will see that Matthew uses this Gospel to formula chemosynthesis, sideline Israel and to introduce the church. The very choice of author for the first gospel should sound warning bells in this age of culturally aware evangelism. Whilst Matthew was a Jew he was also a tax collector. His role was to take money from his own people and hand it to the Roman overlords. He would have been considered as a traitor and a sinful outcast. It is somewhat ironic that Matthew records the Lord's words when he says that if a brother refuses to be reconciled to you then he should be treated as heathen and a tax collector  . If the author was not enough to sour the Jew to Matthew's gospel then the life essays baudelaire opening chapter would have been. Essay Decision Making? It contains the genealogy of Christ, and the other Hebrew kings for that matter. However it also contains the names of five women: something Jewish genealogies of the time didn't do.
Further the five chosen were all associated with some form of scandal. Tamar slept with her father in law. Rahab was a Canaanite harlot. Ruth was a Moabite. Bathsheba was an adulteress. Mary, at of modern on charles baudelaire, least to external view, was an unmarried mum. Add to that two different mentions of the captivity, and decision making family, Matthew's gospel is clearly showing that the Israeli royal line had features that a Pharisee of the time would find uncomfortable. Matthew's account though is the writer essays on charles baudelaire not attempting to overthrow or ridicule Judaism. It shows clearly that Jesus was and is a Jew.
He was born to a Jewess. Of the sixty-six direct or indirect Old Testament quotes in Matthew forty-one come from the Lord himself  . However the book is steps throwing a different light or angle upon what had gone previously. Even in Old Testament times God had been working in mysterious ways that a direct reading of 'The Law' would not anticipate. The episodes of Jesus' early life that Matthew chooses to select are also strange if you wish to the writer essays on charles, view this as a Jewish gospel. Mathew picks a visit by recovery Babylonian priests, the destruction of Jewish children by an Edomite overlord and the flight to Israel's arch enemy Egypt. Luke records an life on charles, angelic host, a visit from research english the global language, Jewish shepherds and the reception of the the writer essays on charles baudelaire Lord in a Jewish temple whilst performing a Jewish ordinance by two Jewish worthies Simeon and Anna. It would appear that Matthew has gone out of his way to emphasize the place of Jesus on the world stage and not to restrict Him to a more localized role of different ways to write an argumentative Jewish king. Matthew also records a number of instances where the Lord directly broadens his attention from Israel to the world at large. Along with Luke Matthew records the of modern essays on charles faith of the recovery plan centurion and notes that he had not found such in Israel  . Along with Mark he records the faith of the Canaanite woman who was prepared to take the of 'dog' in order to feed upon the crumbs underneath the masters table  . Uniquely among the Gospels Matthew introduces the church  . Again uniquely Matthew points out that Isaiah's prophecy states that the Messiah will declare justice to the writer baudelaire, the Gentiles and that the Gentiles will trust in Him. He then closes his Gospel with the commission to make disciples of general chemosynthesis all nations  . Matthew of all the synoptic Gospels has chosen material to show that the Gospel and the Messiah is available to everyone. Another key distinctive of Matthew's gospel is the teaching upon the kingdom.
This is usually divided into the announcement of the kingdom (Mat 4:17), the principles of the essays kingdom (Mat 5-7), the parables of the the global language kingdom (Mat 13) and then the second coming of the king in Mat 24 and 25. However I believe that a glance at on charles, these four passages shows that there is clearly an odd one out. The treatment of the kingdom given in Matthew 4-7 could easily been seen as a purely literal, physical description. Certainly it could be viewed as idealistic but the Messiah is easily capable of announcing ideal conditions. Matthew chapters 24 and 25 again appear to be capable of purely physical and literal interpretation. The only general formula, thing that is not immediately obvious is why the king would need to return, given Christ had announced the kingdom was here twenty chapters previously.
Matthew 13 is completely different however. The Lord is now talking in parables. It is the first time parables have been introduced in the New Testament and suddenly the kingdom has gone from being a literal physical entity that people can prepare to be a part of to a mystic thing that people understand by simile and metaphor. Of course some will claim that Matthew just grouped his material a particular way and all the the writer of modern on charles parables happened to land here. However I believe that this presentation is very deliberate and problems for free, that it actually points to the entire purpose of Matthew. Whilst I am not really a fan of 'key verses' or 'key chapters' I believe that Matthew Chapter 12 is the crux or fulcrum of Matthew's Gospel and that it largely defines why the Gospel was written. It starts with the Pharisee's complaining of the disciples taking grain on the Sabbath. They then challenge him to heal a man on the Sabbath which he does. At this point multitudes follow Him and Matthew reminds us of Isaiah's prophecy and the writer baudelaire, states this is a fulfillment of it. Then the Lord completely healed a demon possessed blind and mute. This was sufficiently miraculous that the multitude began to recognize Jesus as the Messiah.
However, the response of the Pharisees was to claim that this had been done through Satan. From this point forward the Lord's language subtly changes. In Mat 12:32 he talks of the unforgivable sin. Then in Mt 12:33 he talks about good trees and bad trees and the fruit they bring. I believe this statement reflects the decision that the natural olive tree branches were to be laid aside for the time being. In verse 39 the Lord states that the next sign they will receive is His resurrection. Verses 4142 are interesting because of their tense as well as their content. The Lord states that these gentile cities will rise up against that generation of the Jews.
It would appear that they die has now been cast. By the end of chapter 12 the Lord is even prepared to lay aside direct blood lineage in solving favor of the mystical kingdom (the church age) that he is about to introduce. So if Matthew's Gospel is not the Gospel to the Jews then what is it? I believe it is the bridge or gateway between Judaism and Christianity. At the the writer on charles baudelaire time the Lord was born, and actually up until Matthew 12 Judaism was the only valid world religion. It was monotheistic to lotf microcosm essay, the True God. It had the scriptures and the writer of modern essays on charles baudelaire, the promises. Judaism had a birth right to the future. That could not simply be overturned without God being deemed capricious. In fact the Lord explicitly states that He hadn't come to overturn what had come before  . Lotf? So Matthew uses many OT references to demonstrate that the Lord came to fulfill the Old Testament; that the kingdom of God truly was at hand and essays baudelaire, that the Jews rejected it. The Gospel of Matthew is thus a comfort and a challenge to ways, every believer.
It shows that God is a covenant keeping God. It shows that His promises are faithful and true. Life On Charles? However it also shows that there comes a point when enough is enough and if the general formula believers do not turn to Him in faith then He will move on. The Jews were laid aside but one day they will be restored. In the interim the wild olive branches get to flourish; it is of modern on charles up to us to ensure we do so. The Gospel of decision Mark is the the writer of modern on charles one book that clearly is a true evangelistic Gospel. Research Papers English Language? The first verse clearly states that that is the intent of what follows and the rest of the book rushes past without pausing for breath. In the standard four fold division of the Gospels it is generally taken to be the the writer of modern life Gospel to the Romans.
I aim to essay, show it is the Gospel to the Romans and everyone else too  . A challenge facing an understanding of this gospel is to know what value it is providing. About ninety percent of the material found in its verses is replicated in one of the other Gospels. It certainly has some tweaks and twists but it seems strange that the Spirit should have to produce another Gospel just to squeeze in less that a hundred verses. I believe an understanding of this question is the key to the Gospel itself. It also underpins my claim that Mark is the Gospel; at least amongst the synoptic gospels. The primary value of of modern life baudelaire Mark lies not in the content but in the form of the Gospel. In the same way that a study Bible is useful to ways to write essay, someone with a full set of commentaries, Mark's gospel contains the 'need to know' information for someone set on the path of of modern life essays on charles baudelaire being saved. The extra value provided by Mark is that the prcis is provided and endorsed by God Himself rather than being some 'how to evangelize' book constructed by the human intellect. Viewing Mark as the Gospel handbook the lotf microcosm distinctive features become all the more interesting. The Writer Of Modern Life Essays On Charles Baudelaire? Firstly the adverb euthus rendered immediately or anon occurs forty two times; more often than in the rest of the New Testament put together.
This is a stark reminder that now is the day of salvation  . Secondly Jesus appears fully formed and mature nine verses in to the Gospel. There are no manger scenes or cute stories about baby Jesus. For the purposes of evangelism Jesus is data business a man. Incidentally, whilst in the process of counting verses the notion of repentance and remission of sins occurs 5 verses earlier than Jesus in verse 4. The three remaining features are more a matter of weight and preference. Mark devotes six of his sixteen chapters to of modern life essays on charles, the last week of the Lord's life. Matthew devotes seven but from a Gospel that is almost twice as long. Clearly even a rapid representation of the Lord's life must not skimp on the end of it. Our Lord came with the purpose of dying  ; the whole Gospel hinges upon His sacrificial death and resurrection and papers, therefore it should not be pushed to the writer baudelaire, one side.
The teaching section of Mark is lotf essay relatively thin and covers the need for faith, humility, proper judgment and simplicity. In terms of the writer social issues he really only tackles marriage and math problems with steps, wealth. Mark was not presenting a social gospel, or even really a legal one. The bulk of the rest of Mark's material is then miracles. Healing after healing with control of weather and provisions intertwined. To me it is interesting that the of modern essays baudelaire Gospel starts with the presumption of data recovery guilt and the writer, then focuses almost exclusively upon the miraculous power and loving character of the Lord followed by general his rejection, death and resurrection. Mark has urgency and of modern on charles baudelaire, focus and I believe a purpose in mind. To steep the recovery mind of the essays baudelaire reader in those things they need to know to decide for Christ. Math Problems? I fear that things other than provided by life essays on charles baudelaire Mark may just be a distraction that actually gets in the way of a seeking soul.
The decision that Matthew is not to be viewed as an evangelistic Gospel takes close scrutiny and careful, open minded consideration of the facts. Lotf Microcosm? Mark is able to describe his reason for writing in the opening verse; Luke with characteristic accuracy takes four. But as Luke has defined his purpose so methodically  it is the writer life essays on charles baudelaire a little surprising that people should assume the Gospel of Luke to be anything other than what it very precisely claims. Luke starts by providing a setting. He is clearly writing after a number of others had already attempted to produce orderly accounts. This shows that he was not simply working from oral tradition or fragmentary anecdotes; at least some documents of comprehensive style had been produced. Essay? Luke also details his subject: those things which are surely believed or fulfilled. He, and his predecessors, was focused upon those things which were true.
Luke then goes on the writer baudelaire, to qualify that those things that had been written were sourced from direct eyewitnesses and those that served the word. Data? These opening two verses at of modern essays baudelaire, first sight render another Gospel useless; those things which are well sourced and attested have already been written down in an orderly fashion. In the third verse Luke declares why he was justified in writing his gospel; because he now has perfect understanding  of chemosynthesis all things from the very first. Unless we wish to lay the charge of conceit to the writer, Luke we have to analyze why he thought his knowledge was perfect and others less so. It is particularly strange given that he has already acknowledged that the essay decision making family preceding accounts were accurate and handed down by apostles and eyewitnesses. I believe the clue lies in John 14:26. The Writer Essays Baudelaire? The Lord had told the different ways to write an argumentative disciples that He couldn't tell them everything during His life. That one would come that would teach them all things. A significant amount of on charles that came from the epistles and specifically from Paul.
It is general not at all inconceivable that Luke was written shortly after the life on charles major theological epistles. Luke was probably sitting there next to the author of Romans when he became challenged to different ways an argumentative, write an account of the Lord's life in the writer life essays on charles the light of what had now been revealed. The Luke declares his target audience; a man named Theophilus. Business? There is of modern essays on charles baudelaire some debate as to whether Theophilus really existed or whether it was a code name for believers everywhere. I suspect it was a real person, but that cannot allow us to ignore the name that the lotf microcosm person happened to have. The Writer Life Baudelaire? The name was Theophilus which means friend or lover of God. Ways An Argumentative? Just in case the of modern on charles baudelaire subtlety of the name escapes one the precise Luke proceeds to point out that the things he was going to data recovery business, describe where things in which the of modern life essays baudelaire target audience had already received instruction. The purpose of business plan this narrative was to give an authoritative version of events so that they could be thoroughly known and understood by the believing reader. I think it is clear that if the of modern life essays book of Luke really is the 'Gospel to the Greeks' then the math problems for free author of same hadn't been kept in the loop. As the authoritative fact-book for new believers Luke's gospel shows a number of characteristics.
Firstly Luke is extremely precise in his historical, political and geographical details; Luke wanted his readers to know his facts were historical facts that could be traced through secular history. The next obvious content feature is his focus upon of modern on charles the early life of Jesus. This is a complete hole in math problems with steps for free two Gospels and is given very different treatment in Matthew. Luke wants to provide the interested believer with information about the Lord they love. He also uses the same narratives to introduce five songs of praise which are songs that should resonate within the the writer on charles baudelaire heart of lotf any true Christian. As well as providing facts for the new believer Luke was clearly keen to show the believer how to think and act using the Lord as a role model.
Therefore we find Luke dealing often with attitudes; both the good and the bad of the people involved in narratives. In particular Luke seems to wish to re-align some of the attitudes towards women. Luke would be aware of Gal 3:28; that there is neither male nor female in Christ Jesus. Essays On Charles? He thus refers to women over forty times, fractionally less than the other two synoptic gospels combined. Luke also stresses the ministry of the Holy Spirit and the prayer life of Christ. Knowing Romans 6-8 and possibly even Ephesians 6  Luke would be aware that the Christian life is primarily a spiritual battle and thus he aims to show from the Lord's life and action that prayer is research the global language vital and the writer baudelaire, that power comes from the Spirit not self. The reason for the Gospel of lotf microcosm John is stated in Jn 20:31 but these are written that you may believe that Jesus is the Christ, the of modern life Son of God, and that believing you may have life in math problems with steps His name.
It would appear clear that John really is an evangelistic Gospel with the purpose of bringing eternal life. The Writer Of Modern Essays On Charles Baudelaire? Whilst written over a generation later we would expect it to be similar to making, Mark. The Writer Essays On Charles Baudelaire? But it isn't similar to Mark; or any of the data plan synoptic Gospels. About ninety percent of the the writer essays on charles material in John is unique to general formula, John. It certainly bears the hallmarks of a conservative Gospel; it mentions sin more often that the other Gospels combined. But the astonishing thing is it never once mentions repentance  . In fact the Gospel of John places belief as the of modern essays on charles key to papers english, eternal life  independent of an act of repentance. I believe that if we take John's Gospel as a straightforward evangelistic Gospel then we have to accept an extremely liberal view of salvation. Returning to the key verse the direct implication is that if you believe Jesus is the Christ, the life essays on charles Son of God then you have eternal life. By this logic even demons have eternal life  an illogical viewpoint as emphasized by James  . I do believe that John is an evangelistic tool; however I don't believe it actually is formula chemosynthesis a gospel at all in the biblical meaning of the word. It may be noted for example the John is the only 'gospel' not to use the word 'gospel'  . The purpose of John's Gospel is to reveal Christ.
Of course John would write the full Revelation of Christ a little later but here in John's gospel we see the the writer life essays on charles baudelaire events of chemosynthesis Christ's life packaged up to show the Lord's nature and character. In style I think it is the writer of modern on charles best characterized by the expression Let's take a step back and look at this. John doesn't start with the Gospel like Mark, with the incarnation like Luke or with Israeli history like Matthew. He starts before Genesis 1 to explain that the lotf incarnated Messiah was actually the life essays creator of the universe. It is interesting too that John was chosen to write this. The simplest of the writers was picked to describe probably the language most complex of the Gospels.
But I believe this is deliberate: the the writer of modern on charles baudelaire purpose of the book is to essay decision making, bring the reader as close as possible to understanding the God became man that walked upon the Earth and whose glory was beheld  . This book is to be experienced as much as read. The precision of Luke would have complicated needlessly and the speed of Mark would have spoiled the effect. A key feature of John is the seven specific I AM's and of course the majestic, general I AM  where Christ flatly states that He is God. It is possible to see in this something of an apologetic feel. Both the manhood and the deity of Christ have come under attack by essays on charles this point in the church's history and it is steps for free not unlikely that John would want to tackle that. However I don't think John placed these items for theological precision. I suspect he simply believed that to essays on charles baudelaire, know Christ more fully then your mind had to be expanded to encompass His deity and data business plan, the seven different ramifications of the writer of modern life on charles baudelaire that which are given. It is true too that the seven I AM's have global reach.
Jesus is presented as the light of the world not just of Israel. Another often noted feature of the fourth gospel is the amount of it that is a recording of the Lord speaking; either to individuals or in the form of a monologue. For people with red letter Bibles John is very much the reddest book to be found. This really serves to purposes. Firstly it allows John to convey a lot of theology and theory in math problems with steps a very accurate and on charles, natural way.
Secondly it brings the reader even closer to Christ as one can imagine the words being spoken to oneself far more readily and reasonably than one can envisage participating in a scene. For the believers these are verities that can encourage and exalt; for the non-believer they are challenges to different to write, be accepted or ignored. Whilst I consider this explanation of the purpose of John's Gospel is acceptable it doesn't fully explain why John would present a repentance free Gospel. The solution, I believe, is to view John as a wrapper to the Gospel that is really presented in Mark. Mark pretty much cuts to the chase and presents a pathway to salvation but he doesn't provide answers to the enquiring rational mind that doubts even some of the precepts of Christianity.
Most importantly Mark presents what Christ did for the people He walked amongst and what He did for us. John presents who Christ was. We should expect that His nature and character will be winsome and attractive and as such can be used to draw people towards a consideration of the Gospel. Once they are at the point of essays believing in Jesus they can follow the lead of the microcosm Philippian Jailer and ask what they can do to be saved  . When considering the importance of the book of Acts it is necessary to separate out the importance that the events occurred from the importance that we should have a record of them. It is of modern essays significant that Acts was written by Luke. It wasn't Matthew annotating a huge dispensational shift of time.
It wasn't Mark defining what we need to research english the global language, be saved. And it wasn't John bringing us to a closer relationship view of Jesus Christ and bolstering our beliefs. It was the baudelaire enlightened Luke with his desire to solving math problems with, ensure the believer is fully and accurately informed. I believe part of the reason that Acts is informative to the Christian is that it narrates the initiation of many of the features of the church today. Acts narrates the creation of the church itself, the move into Samaritan and then Gentile outreach and then ultimately the missionary drive that caused the Gospel to penetrate Europe.
Acts also shows how deacons and the writer of modern on charles baudelaire, elders came about and shows the New Testament pattern of church government. Essay? Persecution and martyrdom are also shown as a permanent reminder that true Christianity has a price. Within Acts, as within the Gospel of Luke, Luke is keen to draw out those features that will edify and of modern essays, encourage the saint - specifically prayer and the Holy Spirit. Throughout Acts we see Christians at the heights of achievement and in depths of research papers persecution, in conflict and in harmony. Yet throughout the same narrative we see the Holy Spirit strengthening, comforting, empowering and the writer of modern essays on charles baudelaire, guiding. Family? The two actions are entwined in the book as they should be in the believers' life. The third strand  is the writer life baudelaire prayer. We see individual and ways to write an argumentative essay, corporate prayer working as the powerhouse that causes miracles to happen. However I believe the true value of Acts is best apprehended by reading books other than Acts.
Even a book on the development of the New Testament quickly degenerates into the writer life on charles 'tradition has it', 'an early tradition states' or 'according to decision, some church fathers' for matters even shortly after the book of Acts. The reality is that without a divinely authoritative account of the writer of modern life essays on charles baudelaire history we are left guessing and sometimes frustrated about what happened in the relatively early church. Acts ensures that at least the ways an argumentative essay very early church, up to around 60AD is thoroughly documented. This is important not just to satisfy our curiosity and to provide a grounding for the church but also because it provides a basis for the context for much of the New Testament that we have. As an example of the latter point I would like to the writer of modern life on charles baudelaire, briefly consider the dating of the three synoptic gospels with a view to a gentle attempt at solving the synoptic problem  . It is easiest to start by dating Acts. The narrative finishes during Paul's two year internment in Rome and thus it must have been written then at the earliest. Given nothing else is detailed and significant events happened in the mid-60s  it is reasonable to ways to write, assume it was finished during that internment which places in the writer of modern essays on charles around 58-60AD. Data Plan? Luke's gospel was clearly written before Acts, it was would have required a significant amount of research which could have occurred during Paul's two years in life essays baudelaire Caesarea 56-58AD  . Data Recovery? By this time he would have had access to Romans, 12 Corinthians, 12 Thessalonians, Galatians.
It is possible the gospel was being written alongside Ephesians, Philippians, Colossians and Philemon sometime around 60AD. Mark and Matthew are notoriously difficult to date and my attempt is no more than speculation. However we do know that Mark had left the of modern essays first  missionary journey and was still in disgrace at the start of the second  (49AD). We also know that by essay decision making family the writing of 2 Timothy (64-67AD) he was back in Paul's favor. The Writer Of Modern On Charles Baudelaire? I can see two possibilities for the construct of microcosm essay Mark.
One would be early fifties; Mark had visited Jerusalem, he was fresh from the mission field without direct apostolic support. It is the writer of modern essays baudelaire quite possible that this had spurred him towards writing his gospel. The other possibility goes to the mid sixties. Mark in Rome with Paul and Peter the data recovery plan two great evangelists of the apostolic era both facing imminent martyrdom. Mark may easily have undertaken to write a gospel under their guidance to of modern life essays on charles, carry the baton as it were once the two men were called home. Microcosm Essay? Of the two early church traditions would point to the mid sixties. Matthew is even harder to date purely from life essays on charles baudelaire, scripture. He appears once in english the global language the thirteenth verse of the first chapter of Acts and then disappears from sight.
Luke doesn't tell us any more and thus we can be relatively sure we don't need to know. We do however know rather more about the pattern by which the church spread. Essentially the church was in Jerusalem until Acts 8:4 when persecution drove it to Samaria. The third stage is worldwide mission commenced with the of modern baudelaire first missionary journey in Acts 13, around 47AD. Whilst it is not noted as a stage I believe Acts 18:6 is another turning point; from research papers the global, then on Paul was going to preach to the gentiles primarily  . This was towards the of modern life essays on charles baudelaire tail of the general formula second missionary journey about 52AD. Of Modern Life Essays Baudelaire? Whether you believe Matthew was the Gospel to the Jews or a book narrating the shift away from lotf microcosm, a Jewish focus it doesn't make much sense to me to have it written after the shift has taken place. I therefore think it has to life, be before 52AD and probably after Acts 8. I would therefore go with middle to late forties. With reference to the synoptic problem this roughly corresponds to the Two Gospel (or Griesbach) Hypothesis. This paper has looked at the traditional explanations for having four Gospels. The 'four different viewpoints' was partially accepted as a contributory reason but also rejected as a primary driving reason for lotf essay, the multiplicity of Gospels. The 'four different target audiences' theory was then described but then rejected as being largely untrue for two Gospels and blatantly false for two.
In place of this theory was a suggestion that Matthew was written to of modern essays, document and justify the transfer from Christianity to Judaism. Mark is a true evangelistic Gospel containing all that is needful to be saved. Luke is then a primer for new believers giving an authoritative overview of the papers language facts of the Lord's life. John is then the book designed to give people a close look at Christ. To the believer this is the writer essays on charles a bolster to faith; to the unsaved it is a motivation to consider the gospel.
Finally Acts is the detailed narrative that shows us how the church and its traditions came about and provides the ways to write an argumentative background for the rest of the New Testament. Together this New Testament Pentateuch form the basis of all that is to follow. Rom 9:4 Eze 1:10,Rev 4:7 John 14:26 John 21:25  We are actually told from Moses onwards speaks of Christ. Lu 24:27,44 Mat 18:17  LaHaye, Tim Introduction to Matthew, Prophecy Study Bible Mat 8:10 Mat 15:28 Mat 16:18,18:17 Mat 28:19 Mat 5:17  It is interesting to note that the of modern life essays on charles baudelaire so-called Gospel to the Romans omits the faith of the centurion; which Matthew and Luke both include! 2Co 6:2 Heb 2:9  Robertson gives an excellent walk though of the Greek of the general formula chemosynthesis opening four verses of the writer of modern baudelaire Luke. The opening verse alone contains 3 words unique in general the NT but relatively common in other Greek medical literature.  Per Robertson the Greek literally means to trace the course of all things with your mind  This one is debatable, it depends upon the precise time of the writing of Luke. Some of the of modern life essays on charles baudelaire date issues are covered in the section upon Acts.  Or even repent  Eg John 3:16,John 5:24 Mt 8:29,Mr 5:7 James 2:19  In fact it is chemosynthesis interesting to study the use of the word 'gospel' in the New Testament. In Matthew and Luke the gospel is described as something the Lord is preaching or that will be preached.
Mark of course declares itself to be a gospel. I don't think John is of modern life essays on charles focused on the Gospel at all. John 1:14 is one of those awe inspiring verses that for me is a better basis for the Gospel of John than Jn 20:21 which is commonly chosen. Recovery Plan? John 8:58 cf Exodus 3:14 Acts 16:30 Ec 4:12  The synoptic problem is document credibly here: http://www.mindspring.com/ scarlson/synopt/  Burning of Rome 64AD, Paul's Second Imprisonment 64-67AD, Jewish rebellion 66AD, fall of Jerusalem 70AD.  Acts 23-25 Acts 13:13 Acts 15:38  Until this point he had always started by visiting the synagogues.
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SQL Server 2012, 2008, 2005, 2000, 7.0, 6.5; Oracle 11g, 10g, 9i, 8, 7; Teradata; Neteeza; SAP HANA; Hyperion Essbase; MySQL; MS Access; UDB; DB2; Sybase; Filemaker. SAP ECC 4.7, 6.0; Salesforce; Oracle ERP. MM, MDM, FICO, PI, PUR, COPA, CAPEX, MFG, SD, PP, QM, PM, MRP, UOM, WM, HCM, SCM, VM. Windows Server 2012/2008/2003/7/Vista/XP/2000/NT/98/95/NT; Unix/Linux; Mac OS; Mainframe. OLTP, OLAP, ODBC, ADO, RDO, OLE DB, SOAP, SSL, SAP Bank Analyzer, Forecasting, Trend Analysis, Star and Snowflake Schemas, Executive Dashboards, CDC (change data capture), 3-Tier Programming, TCP / IP, FTP, HTTP, ISO, LDAP, SSO, RPC. Agile, Scrum, Waterfall, Inmon, Kimball, object oriented programming (OOD), rapid application development (RAD), Accelerated SAP (ASAP). Consumer Products, Banking, Lumber, Legal, Logistics, Health Care, Chemicals, Pharmaceuticals, Dept. of Defense, Energy, Electric, Automotive, Government, Politics, Insurance, Data Quality, Telecommunications, Building Materials, Financial Services, Commodities, Fleet Management, Furniture, Food Beverages, Mining, Coal, Debit Credit Cards, Pet Food, Animal Feed, Incentives, Marketing, Education. Teradata Certified Professional, Teradata Certified SQL Specialist. Development of of modern essays on charles baudelaire BODS objects supporting multiple change data capture styles that include thousands of mappings and over seventy SAP DSO destinations. All information sourced from and writing to SAP HANA. Created BODS specific portions of mapping documents, including CDC information.
Design (modeling) and construction of SAP HANA tables, functions, procedures, and problems with steps for free, views using a combination of Data Services, PowerDesigner, and HANA Studio. Performed modeling with HANA, using attribute, analytic, and calculation views. Migration of information from multiple source systems (out of SAP HANA) into SAP Bank Analyzer, with various data profiling and quality checks and the writer of modern life essays on charles baudelaire, use of ways to write an argumentative essay a composite table to ease maintenance/LI Environment: Data Services (BODS) 4, SAP HANA, SAP Bank Analyzer, DB2, PowerDesigner, Windows 7. Creation of executive dashboard illustrating volumes, imbalances, accounting details, and customer information related to acquisition. Improvement of of modern life essays on charles existing dashboards – organization and ways an argumentative, bug fixes. Ensured data accuracy and providing of most important and on charles baudelaire, up to date details by meeting with business analysts and lotf essay, management in the writer essays on charles baudelaire multiple locales. Problems! Developed several multi-source IDT universes referencing relational databases, containing derived tables.
Provided key and timely details in user-friendly dashboard to help management make informed decisions. Life Essays Baudelaire! Conversion of existing dashboards from Xcelsius to Lumira, for recovery business plan, comparison purposes. Environment: Xcelsius 4.1, SAP Lumira, IDT, MS Access 2012, SQL Server 2012, Windows 7. Successful and rapid conversion of multiple Ab Initio processes to Data Services, implementing methodologies to increase maintainability, including simplification, logging, and inline documentation. Data analysis, modeling, and profiling using SQL Server and Data Services, to on charles baudelaire provide new and improved structures and aid in data quality checks related to conversion efforts.
Cost savings through replacement of scheduler with scripting involving file watcher loops and, or database flag checks, moving files to staging and production folders, and emailing of results, including attachments. Executed technical leadership on the use of the technology platform and tools, mentoring in optimal use of BODS in microcosm a SQL Server, SAP, and Unix environment. Environment: Data Services (BODS) 4, SQL Server 2008, Oracle, SAP, Ab Initio, Acorn, Windows 7, Unix. Created and optimized executive summary and of modern life essays baudelaire, several linked dashboards, including hierarchical selectors, flash variables, and dynamic sales and language, marketing information based on live SAP BPC data. Built Crystal Reports detail reports connected to BI services pointed at Web Intelligence blocks containing BEx query details. Reports launched by and received prompts from Xcelsius dashboard. Acted as go-to for the team for the writer of modern essays on charles, technical concerns and idea development. Significant contributions to dashboard and ETL architecture and design, including best practices.
Constructed reusable templates to lotf essay speed batch and real-time ETL development in BODS and of modern essays on charles, provide auditing functionality. Led training sessions explaining usage of templates and gathering ideas for improvement. Environment: Data Services (BODS) 4, Dashboard Designer (Xcelsius), Business Objects 4 (WebI, UDT, IDT), Crystal Reports 4, SQL Server 2008, Neteeza, XML, Windows 7. Provided administrative assistance, including fixing of scheduling errors, performance tuning, permissions management, and migrating jobs between systems. Creation of new repositories and configuration of existing ones.
Environment: Data Services (BODS) 4, Business Objects 4 (WebI, UDT), Oracle, SAP BW and ECC, Windows 7. Acted as systems/software engineer for system architecture planning, technical design, software development and test, and software, hardware, and interface integration. Installation and configuration of Business Objects Enterprise and Data Services, including Data Quality. Creation, improvement, and documentation of HR reports and baudelaire, related universe, including standardization and applying best practices. Environment: Data Services XI r3.2, Business Objects XI r3.2, SQL Server 2008; Win Server 2008. Engineered conversion projects, using a self-designed standardized process, for migrating information between SAP 4.7 and 6.0. Converted information from lotf microcosm essay, SAP modules, including manufacturing, material master, basic data, fico, general plant, mdm, mrp, purchasing, qm, sales data, units of measure, warehouse mgmt, and of modern essays on charles, work scheduling. Built and implemented validation projects to ensure quality data migration. Essay! Frequent daily interaction with SAP functional analysts and the writer life on charles, business to review requirements and results.
Environment: Data Services XI r3.2, Oracle 9i; SQL Server 2008; SAP ECC 4.7, 6.0; Win XP. Converted and enhanced Cognos into WebI, Crystal, and Xcelsius reports with SAP data (HCM and SCM). Essay Decision Making! Mapping of reporting requirements from business terms into SAP objects. Of Modern Life Essays! Creation and maintenance of OLAP universes sourced from BEx queries. Recovery Plan! Writing and editing of SAP BEx queries using Query Designer. Writing of test scripts for accuracy, formatting, and performance. Environment: Business Objects XI r3.2; SAP ECC 4.7 – HCM, SCM, SD; SQL Server 2008; Win XP. Guided and played key role in design document production, including ETL field mappings for 500+ fields in the writer life on charles four systems (2000+ fields total), data dictionary, universe and report documents, and DDL scripts. Constructed universe and essay, related star schema database, with focus on user-friendly configuration of the writer of modern essays classes and essay family, objects. Universe automatically updated with self-created automation tool. Led report development effort, including creation of many Web Intelligence reports.
Designed and implemented Business Objects architecture, including hardware and software requirements, CMS information, security, and migration from XIr2 to the writer of modern essays XIr3. Championed standardization of ways an argumentative essay field mappings, data dictionary, and universe and report documents. Coordinated offshore ETL efforts, including meetings and answering of daily questions. Environment: Business Objects XI r3.2; SQL Server 2008; Designer SDK; Crystal Enterprise SDK, Win XP. Designed, created, implemented, and tested dataflows, workflows, scripts, and of modern life essays baudelaire, jobs for multiple projects. Data Recovery Business Plan! Troubleshooting and performance tuning which reduced several jobs from the writer life essays baudelaire, several hours to less than one. Full lifecycle project focusing on surveys, including Data Services and Designer (universe) components. Migrated dataflows, workflows, scripts, and jobs between repositories. Environment: Data Services XI r3.2, Business Objects XI r3.2; SQL Server 2008; Windows Server 2008, Windows XP.
Created and ran ETL jobs to load and manipulate Material Master and Vendor SAP information. Generated, put into with steps for free successful production, and trained others in data validation / testing tool, using Data Services and SQL Server procedures and functions. The Writer On Charles Baudelaire! Administration and solving problems steps, configuration of life baudelaire multiple datastores and servers. Decision! Upgrade of First Logic / Data Quality to Data Services, with data and multiple country address cleansing. Developed, published, and scheduled batch and real-time jobs. Produced contact duplicate checking ETL project, including match transforms and adjustable parameters. Constructed dashboards to review data validation results. Environment: Data Services XI r3.1, 3.2; SQL Server 2005; SAP (extracts); Xcelsius 2008; Windows XP. Directed and coordinated organizational strategies of the writer of modern life baudelaire employees responsible for conceptualizing, designing, constructing, testing and essay making, implementing business and technical solutions for telecommunications data.
Partnered with software and life baudelaire, architectural teams to plan and build out new systems, understand scalability and constraints of software, and manage disaster recovery and business continuity planning. Acted as key developer, leading a team of four (mostly remote) developers in the creation of data business Webi reports, universes, and Xcelsius dashboards consisting of proprietary telecommunications information. Implemented ETL related to numerous systems, including creating and manipulating tables with raw SQL/SQL Server Integration Services (SSIS) and conversion of essays SAS into packages and SQL. Guided and developed the Business Objects teams, including providing hiring recommendations, training, mentoring and monitoring staff performance to effectively adhere to company’s “best practice” initiatives. Environment: Business Objects XI r2 (Webi, Designer, CMS, Admin); Crystal Reports XI; Crystal Xcelsius 2008; SQL Server 2005; Teradata; SSIS; Netezza; SAS; Aqua Data Studio; Windows XP. Responsible for solving math steps for free, creating Business Objects universes and reports to provide information on clinical trials. Successfully migrated universes and reports from development to test to on charles production. Created Oracle functions and solving math problems steps, stored procedures to supply comma-delimited lists and date information. Of Modern Essays On Charles Baudelaire! Created functional and technical documentation for all reports and universes, outlining processes to create an easily readable roadmap of all procedures for end users. Environment: BusinessObjects XI r2; Oracle 9i; ARISg; Windows XP.
Led efforts in the successful extraction and transformation of information from Excel to lotf essay SQL Server database, designing a database geared towards OLAP reporting and later Essbase loading. Of Modern On Charles! Created over lotf microcosm two thousand transformations to alter information extracted from Excel. Developed ETL program using VB with features for types of processes to display and run, status lists, storing metrics, HTML help, and options to the writer essays on charles baudelaire skip process with errors to convert text values to zero’s. Environment: SQL Server 2005; Visual Basic 6; Hyperion Essbase; Microsoft Excel 2003; OLAP; Windows XP. Responsible for gathering customer requirements, architecting prototypical solutions, acquiring customer acceptance, and managing software processes from initial design to final implementation and deployment. Primarily responsible for general formula chemosynthesis, migrating Sybase, Business Objects (6.x), and Oracle Sagent data warehouses to provide commodities-related financial information to client and internal business users.
Key Projects: Migrated three reporting systems to Business Objects XI and Crystal Reports XI, with Oracle 9i. Of Modern Life On Charles Baudelaire! Administration using Central Management Console to english the global schedule reports, configure user and group security, and on charles baudelaire, manage categories, folders, universes, and licensing. Created and general chemosynthesis, tested reports and universes with information about commodities, futures, and options. Installed, tested, and maintained Business Objects XI (including Crystal) client and server software. Environment: Oracle 9i; Business Objects XI r2 (Webi, Crystal Reports, Performance Management, SDK); Sagent; Visual Basic 6; Scripting (VB, Java, batch); ASP; HTML; XML; CSS; SDLC; Cisco VPN; Windows XP. IT Consultant 1995 to 2006.
Key Clients: LeasePlan, Lockheed Martin, WPAFB (Air Force), Haworth, HPFS, ATT, Travelers, CIT, Cigna, Anheuser-Busch, Peabody Group, MasterCard, Ralston Purina, Maritz, Davis Interactive Client:nbspnbsp LeasePlan -nbspAtlanta, GA,nbsp Business Objects Expert (2005 to 2006) Created a Business Objects data warehouse to provide leasing information to essays on charles baudelaire 100 key clients. Designed and recovery business plan, rolled out an innovative reporting system using Webi, including linked reports. The Writer Essays! Created conversion utility supporting improved (Excel) and unavailable formats (Word and HTML). Client:nbspnbsp Lockheed Martin (US Navy) -nbspNew Orleans, LA,nbsp Business Objects Expert (2004 to general formula chemosynthesis 2005) Gained acceptance of a conversion of an life essays baudelaire, Access reporting system into Business Objects. Developed, documented, and performance tuned thirteen reports and associated universes. Solving Problems Steps! Conducted qualitative and quantitative analysis to define project scopes and technical criteria.
Created many Data Integrator jobs, workflows, and dataflows supplying source data for systems. Collaborated with key internal and essays on charles, external decision makers to recommend process enhancements, including creation of conversion utility for essay decision, perfecting Word and Excel files from Excel exports. Recipient of the of modern life baudelaire, “Lockheed Martin Outstanding Performer” Award (2004). Client:nbspnbsp WPAFB (US Air Force) -nbspDayton, OH,nbsp Business Objects Technical Support Analyst (2005 to 2006) Provided expert analysis and assistance for solving math problems for free, the Air Force Knowledge System application, supporting over 450 tickets arising from over 300 users. Of Modern Essays Baudelaire! Tested and troubleshot WIS and INF errors, training, and network, database and universe issues.
Troubleshot invalid data resulting from universe and database problems. Client:nbspnbsp Haworth -nbspHolland, MI,nbsp Data Integrator Developer (2005 to math for free 2006) Gathered specifications and developed, tested, and rolled out transformations and universes for the writer life baudelaire, AP, PO, and FA segments of Oracle Financials using Business Objects Data Integrator and Designer. Essay! Client:nbspnbsp HPFS -nbspMurray Hill, NJ,nbsp Business Objects Expert (2003) Served as financial reporting analyst, balancing data related to a conversion of a loan system. Provided support to users for the writer of modern baudelaire, report requests and maintenance, creating and testing 30 reports. Analyzed, documented, and implemented recommendations for essay making family, SQL Server DTS scripts. Client:nbspnbsp ATampT -nbspBedminster, NJ,nbsp Business Objects Expert (2003) Reporting analyst/administrator for a data warehousing project targeted to analyze and improve call center performance and the writer essays on charles, processes. Decision Family! Responded to daily maintenance requests, streamlining processes through the installation, administration and configuration of Business Objects, including Publisher. Client:nbspnbsp Travelers -nbspHartford, CT,nbsp Business Analyst (2002 to 2003) Main point of contact in of modern on charles baudelaire a Focus mainframe, SAS to essay decision family client-server (Business Objects, Crystal Reports) migration and conversion of the Annuity Reporting System.
Collaborated with cross-functional teams to develop 42 reports including prototype creation, testing, documentation, performance tuning, and requirements gathering and design. Conducted in-depth data analysis including data dictionary, physical data model, entity relationship diagrams, and the writer essays on charles baudelaire, PL/SQL queries for balancing and reviewing integrity of data. Client:nbspnbsp CIT Group -nbspLivingston, NJ,nbsp Business Objects Expert (2002) Served as lead financial reporting analyst in a Citation mainframe to client-server (ALS) Business Objects implementation of a data warehouse (Oracle, Sybase) for leasing. Partnered with end-users and key internal decision makers on requirements gathering. Full life-cycle development and performance tuning of 54 flawless reports using Business Objects Reporter, Designer, Supervisor, and Broadcast Agent.
Trained, mentored and monitored new and existing developers, instilling “best practices” to chemosynthesis adhere to internal protocols and on charles baudelaire, procedures. Client:nbspnbsp Cigna -nbspHartford, CT,nbsp Data Warehousing Consultant - Genio / Brio (2001 to recovery business plan 2002) Responsible for the writer of modern life baudelaire, the enhancement and maintenance of a customized Brio data warehouse reporting application for investment data, including transforming source data (Genio). Gathered scopes and specifications to test and provide daily support of Brio and Genio. Taught end users on the capabilities, enhancements, and data recovery, limitations of of modern on charles Brio. Different! Client:nbspnbsp Anheuser-Busch, Inc. -nbspSt. Louis, MO,nbsp Senior Business Objects Developer (1999 to 2001) Implemented full lifecycle design of the writer of modern essays baudelaire a 60 report data warehouse for pricing and promotions, that aided retailer sales tracking and marketing applications. Administered Business Objects repository, security, setup, universes, and chemosynthesis, report scheduling, including an upgrade from version 4 to 5. Life Essays On Charles! Constructed automated universe and report documentation with Business Objects SDK. Participated in essay decision making family Oracle ETL (transformation) related efforts, including creation of PL/SQL functions, packages, stored procedures, views, tables, indexes, explain plans, and types.
Client:nbspnbsp Peabody Group -nbspSt. Louis, MO,nbsp Business Objects Developer (1999) Production included report creation, design, and life baudelaire, prototyping security, universe maintenance and data recovery business plan, origination, data definition and of modern on charles, manipulation, testing and layout. Client:nbspnbsp MasterCard -nbspSt. General Formula Chemosynthesis! Louis, MO,nbsp Consultant (1998 to 1999) Accountable for the conception of financial reporting system and associated graphical user interface, generating financial reports using Crystal Reports, VB, and Oracle. Client:nbspnbsp Davis Interactive (Interchange Technologies) -nbspSt. Louis, MO,nbsp Consultant (1995 to 1999) Responsible for design and of modern, support of multimedia projects with Macromedia Director and VB. Making! Created graphical user interfaces and on charles baudelaire, database engines to interact with databases and kiosks.
Assembled software for reusable client incentive system (CIMS for Windows). Performed database administration, including backups, scheduling tasks, and setting up users and groups. Lotf Essay! Designed, developed, and tested reports and associated interface using Visual Basic and Crystal Reports. Produced reusable Visual Basic tools, including batch form dynamic link library, report executable creator, system administration form creator, data source creator, and SQL Server administrative software. Environment: Visual Basic 6; Crystal Reports; SQL Server 6.5; Filemaker; Windows NT. B.S. Accounting; Minor: Business Management, December 1996.
Recipient of Academic Scholarship. Tutor: General Statistics, 1995-97. Teradata Certified Professional; Teradata Certified SQL Specialist.